Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,679,843 A→C 8.2% intergenic (+62/‑102) bioA → / → rsmE adenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA/16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,679,8430AC 8.2% 144.6 / 4.8 52intergenic (+62/‑102)bioA/rsmEadenosylmethionine‑‑8‑amino‑7‑oxononanoate aminotransferase BioA/16S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

AGCGAAGGCATCGATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTGAGCCTTG  >  NC_002947/5679695‑5679987
                                                                                                                                                    |                                                                                                                                                
aGCGAAGGCATCGATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGaa                                                                                                                                                  <  5:153569/149‑1 (MQ=255)
aGCGAAGGCATCGATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGaa                                                                                                                                                  >  3:117427/1‑149 (MQ=255)
    aaGGCATCGATATCGCCACCCGCGACAGCGGCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGccc                                                                                                                                              <  6:160884/149‑1 (MQ=255)
       gCATCGATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTg                                                                                                                                           <  7:34258/149‑1 (MQ=255)
            gATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCTTCGCCCTTgttcg                                                                                                                                       <  3:109308/148‑5 (MQ=255)
            gATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAgg                                                                                                                                      >  4:156617/1‑149 (MQ=255)
             atatCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCCCACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAgg                                                                                                                                      >  7:29768/1‑148 (MQ=255)
             atatCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGc                                                                                                                                     <  3:182106/149‑1 (MQ=255)
                  gCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACcgc                                                                                                                                <  6:161006/149‑1 (MQ=255)
                  aataCCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACGTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACcgc                                                                                                                                <  8:262949/146‑1 (MQ=255)
                       ccGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGgcgc                                                                                                                           <  3:87933/149‑1 (MQ=255)
                        cgcgACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGcc                                                                                                                          <  4:245133/149‑1 (MQ=255)
                                              tACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCa                                                                                                    >  1:162093/1‑149 (MQ=255)
                                                  ggCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGa                                                                                                >  6:240518/1‑149 (MQ=255)
                                                       aCTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGc                                                                                           <  3:156617/149‑1 (MQ=255)
                                                             aCCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATc                                                                                                  >  3:264309/1‑136 (MQ=255)
                                                             aCCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATc                                                                                                  <  4:264309/136‑1 (MQ=255)
                                                             aCCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTc                                                                                     <  3:134114/149‑1 (MQ=255)
                                                               cccGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGgcgc                                                                                                                           <  6:187065/109‑1 (MQ=255)
                                                               cccGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGgcgc                                                                                                                           >  5:187065/1‑109 (MQ=255)
                                                                       ccGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCca                                                                                  >  8:141273/1‑142 (MQ=255)
                                                                       ccGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCca                                                                                  <  7:141273/142‑1 (MQ=255)
                                                                          cgACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCt                                                                        <  6:152814/149‑1 (MQ=255)
                                                                             cccGGGATAGGCCCGGCAATTCGCCACACCACTTTTACTGTGGGAGCGGGCGCGCCCGAGCACAAGGGCGGAGACCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGCCGCATCGACACGCCCGCTCCCACAGGGGATCTGct                                                                     >  5:219355/1‑148 (MQ=38)
                                                                              ccGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGctt                                                                    <  2:75059/149‑3 (MQ=255)
                                                                                        cccGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGtct                                                                  >  7:1363/1‑141 (MQ=255)
                                                                                        cccGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGtct                                                                  <  8:1363/141‑1 (MQ=255)
                                                                                            gCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCCCCCACAGGGGATCTGCGTCTCTCTATTTACCg                                                      <  2:162093/149‑1 (MQ=255)
                                                                                              aaTTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCCCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAg                                                    >  6:100229/1‑149 (MQ=255)
                                                                                                   gCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAAc                                               <  5:104119/149‑1 (MQ=255)
                                                                                                   gCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAAc                                               >  5:83719/1‑149 (MQ=255)
                                                                                                    ccACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAAcc                                              >  1:33428/1‑149 (MQ=255)
                                                                                                        accacTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATg                                          <  5:100229/149‑1 (MQ=255)
                                                                                                         ccacTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGa                                                                                                >  5:151305/1‑94 (MQ=255)
                                                                                                         ccacTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGa                                                                                                <  6:151305/94‑1 (MQ=255)
                                                                                                         ccacTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATga                                         <  1:203279/149‑1 (MQ=255)
                                                                                                           acTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATgaga                                       <  2:33428/149‑1 (MQ=255)
                                                                                                                  cTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTccc                                >  8:259796/1‑149 (MQ=255)
                                                                                                                  cTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCATGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTccc                                >  7:135014/1‑149 (MQ=255)
                                                                                                                      gggAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGctt                            >  5:207371/1‑149 (MQ=255)
                                                                                                                         aGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGcttctt                         <  5:240518/149‑1 (MQ=255)
                                                                                                                         aGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGcttctt                         <  7:259796/149‑1 (MQ=255)
                                                                                                                              gCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATc                     >  7:190036/1‑148 (MQ=255)
                                                                                                                                   cccGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGcc               >  2:47945/1‑149 (MQ=255)
                                                                                                                                      gcgAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCCCTTCTTCATCGACGccccc            >  5:105351/1‑149 (MQ=255)
                                                                                                                                        tggcAAGGGCTTTGCCCTTGTCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGccccct           >  4:222524/4‑147 (MQ=255)
                                                                                                                                        tggcAAGGGCTTCGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCt          >  3:186976/4‑149 (MQ=255)
                                                                                                                                         ggcAAGGGCTTCGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGt                                                                    <  1:187163/88‑1 (MQ=255)
                                                                                                                                         ggcAAGGGCTTCGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGt                                                                    >  2:187163/3‑90 (MQ=255)
                                                                                                                                         aaCAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTg         <  8:190036/149‑1 (MQ=255)
                                                                                                                                          gcAAGGGCTTTGCCCTTGTCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGccccct           <  3:222524/145‑2 (MQ=255)
                                                                                                                                            aaGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTGAGc      >  8:292351/1‑149 (MQ=255)
                                                                                                                                             aGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTGAGcc     <  6:83719/149‑1 (MQ=255)
                                                                                                                                                gCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTGAGCCTTg  >  7:28808/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCGAAGGCATCGATATCGCCACCCGCGACAGCGTCAGTGTCGCAGTACCGGCCAACTTCCACCCCGACTTCCGCGACCCGGGCTAGGCCCGGCAATTCGCCACACCACTTTTCCTGTGGGAGCGGGCGAGCCCGCGAACAAGGGCGAAGCCCTTGCCAGGCACCGCGGCGCCTGATTCGCGGCGGTTCGACGCATCGACACGCCCGCTCCCACAGGGGATCTGCGTCTCTCTATTTACCGAGCAAACCCATGAGACTGTCCCGCTTCTTCATCGACGCCCCCCTGAGCCTTG  >  NC_002947/5679695‑5679987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑