Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,682,706 G→A 7.4% A1396V (GCG→GTG)  PP_4988 ← signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,682,7060GA 7.4% 153.4 / 2.9 54A1396V (GCG→GTG) PP_4988signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base A (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

GCACCAGCAGCACCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAACGCGCCCCCTTGC  >  NC_002947/5682569‑5682850
                                                                                                                                          |                                                                                                                                                
gCACCAGCAGCACCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCg                                                                                                                                       >  3:107893/1‑149 (MQ=255)
 cACCAGCAGCACCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCgg                                                                                                                                      >  7:204450/1‑149 (MQ=255)
      gcagcaCCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGt                                                                                                                                 <  5:267739/149‑1 (MQ=255)
      gcagcaCCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGt                                                                                                                                 <  1:243040/149‑1 (MQ=255)
      gcagcaCCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGt                                                                                                                                 <  1:121079/149‑1 (MQ=255)
          caCCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCa                                                                                                                             >  8:70607/1‑149 (MQ=255)
            ccGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCAcg                                                                                                                           >  6:35544/1‑149 (MQ=255)
             cGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGTAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCAcgc                                                                                                                          <  4:187873/149‑1 (MQ=255)
             cGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCAcgc                                                                                                                          >  2:60854/1‑149 (MQ=255)
               gCAACGGCACGCTCTGCCCCAGCAGCGCGGGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCAcgcg                                                                                                                         >  1:184190/1‑149 (MQ=255)
                     gCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACACCGCAAGCTCGGCAGGTGGCACGCGGACGATg                                                                                                                  >  4:157718/1‑149 (MQ=255)
                      cACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGc                                                                                                                 >  7:252054/1‑149 (MQ=255)
                       aCGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGcc                                                                                                                >  5:310707/1‑149 (MQ=255)
                         gCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCt                                                                                                              <  7:69840/149‑1 (MQ=255)
                          ctctGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTc                                                                                                             >  6:271572/1‑149 (MQ=255)
                               ccccAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATg                                                                                                         <  1:60854/148‑1 (MQ=255)
                                  cagcagCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCACAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACGTAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTAt                                                                                                     >  7:72265/1‑149 (MQ=255)
                                   agcagcGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACACCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTAtt                                                                                                    <  3:157718/149‑1 (MQ=255)
                                            ggggCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCaa                                                                                                                                            <  7:147396/101‑1 (MQ=255)
                                            ggggCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCaa                                                                                                                                            >  8:147396/1‑101 (MQ=255)
                                              ggCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACGTAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATg                                                                                          <  8:72265/149‑1 (MQ=255)
                                              ggCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATg                                                                                          >  7:244967/1‑149 (MQ=255)
                                                  cgGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGt                                                                                      <  5:271572/149‑1 (MQ=255)
                                                  cgGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGt                                                                                      <  2:184190/149‑1 (MQ=255)
                                                   ggCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCGGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTa                                                                                     <  5:103145/149‑1 (MQ=255)
                                                       ggCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGc                                                                                 <  6:310707/149‑1 (MQ=255)
                                                        gCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGct                                                                                <  7:70607/149‑1 (MQ=255)
                                                         ccTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGctc                                                                               <  8:204450/149‑1 (MQ=255)
                                                               agcaACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGTGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGcc                                                                         <  2:6187/149‑1 (MQ=255)
                                                               agcaACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGcc                                                                         >  5:265260/1‑149 (MQ=255)
                                                               agcaACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGcc                                                                         >  3:119847/1‑149 (MQ=255)
                                                                 caACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGcc                                                                         >  8:111268/1‑147 (MQ=255)
                                                                   aCTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAgg                                                                     >  2:225234/1‑149 (MQ=255)
                                                                   aCTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAgg                                                                     <  7:202229/149‑1 (MQ=255)
                                                                    cTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGt                                                                    <  8:244967/149‑1 (MQ=255)
                                                                     tCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTg                                                                   >  7:63401/1‑149 (MQ=255)
                                                                       gCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACACCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCa                                                                 >  8:7221/1‑149 (MQ=255)
                                                                        cccAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCAc                                                                <  8:252054/149‑1 (MQ=255)
                                                                         ccAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCAc                                                                >  6:137853/1‑148 (MQ=255)
                                                                           aGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACACCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCAt                                                             <  7:7221/149‑1 (MQ=255)
                                                                             gTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCa                                                           <  6:265260/149‑1 (MQ=255)
                                                                                gcgcAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAgc                                                         <  4:119847/148‑1 (MQ=255)
                                                                                      cTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGt                                                  <  8:63401/149‑1 (MQ=255)
                                                                                               gTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGaca                                         <  5:35544/149‑1 (MQ=255)
                                                                                                tGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTTGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGa                                           >  6:12674/1‑146 (MQ=255)
                                                                                                tGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGa                                           <  5:12674/146‑1 (MQ=255)
                                                                                                  gCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACgg                                      <  1:4202/149‑1 (MQ=255)
                                                                                                  gCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACg                                       <  4:107893/148‑1 (MQ=255)
                                                                                                           gACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACgg                               >  5:161781/1‑147 (MQ=255)
                                                                                                                             cAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAAcgcg           >  2:285234/1‑149 (MQ=255)
                                                                                                                               gCTGGTAGCACGACCAAAGCTCGGCAGGTGGCACGCGTACGATGCCCTCGATGCTATTGAGCGGAATGGCGTATAGCTCTTAGCCGAGGTGCACCATCACCGCACGGTTGATCGAAACGGTGAACGGCAGGCGGAACAGGAACCGCGcc         >  2:248485/1‑149 (MQ=255)
                                                                                                                                 tGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAACGCGcccc       >  3:299101/1‑149 (MQ=255)
                                                                                                                                   gTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGAACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAACGCGCCCCCt     >  3:215222/1‑149 (MQ=255)
                                                                                                                                      gCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAACGCGCCCCCTTGc  >  7:94379/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                
GCACCAGCAGCACCGGCAACGGCACGCTCTGCCCCAGCAGCGC‑GGGGCGCGGCAGGCCTTGCAGCAACTCGCCCAGGTAGCGCAGCTCGTATTCGTGGCCGGCATAGACATAGCGCGGCGAGTCCAGCTGGTAGCACGCCGCAAGCTCGGCAGGTGGCACGCGGACGATGCCCTCGATGGTATTGAGCGGAATGGCGTATTGCTCTTCGCCCAGGTGCACCATCAGCGCACGGTTGATCGACACGGTGAACGGCAGGCGGATCAGGAAACGCGCCCCCTTGC  >  NC_002947/5682569‑5682850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑