| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,718,990 | G→A | 7.8% | L563L (CTG→CTA) | PP_5020 → | methyl‑accepting chemotaxis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,718,990 | 0 | G | A | 7.8% | 144.1 / 2.7 | 53 | L563L (CTG→CTA) | PP_5020 | methyl‑accepting chemotaxis protein |
| Reads supporting (aligned to +/- strand): ref base G (21/26); new base A (2/2); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CCCAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGACCTCG > NC_002947/5718842‑5719128 | cccAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTg < 1:136088/149‑1 (MQ=255)cccAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTg < 5:72018/149‑1 (MQ=255) cAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAGTCGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTgg < 1:200803/149‑1 (MQ=255) cAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTgg > 8:137068/1‑148 (MQ=255) agcagTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCAcc > 7:125719/1‑149 (MQ=255) gTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGGTAGAAACCATCACCCGCa > 3:42067/1‑149 (MQ=255) gTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTAGAAACCATCACCCGCa > 3:38017/1‑149 (MQ=255) cGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACg < 8:70291/149‑1 (MQ=255) gACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGTGGGCCAGCGTGATGGCCAACAGCCGCAAACTGACCGACAGCAG‑CGT‑AGAGATGACCCGCCGCGCCGGCACCTCGCTGGAAACCAACACCCGCACg > 1:84318/1‑149 (MQ=255) agaTCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTgtcgtc > 6:167127/1‑148 (MQ=255) cGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATcc < 8:146132/149‑1 (MQ=255) gagTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGc < 5:20614/149‑1 (MQ=255) gagTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGc > 4:114669/1‑149 (MQ=255) ttGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGAt > 1:32832/1‑149 (MQ=255) tGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAAc < 1:161631/148‑1 (MQ=255) cGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcag > 4:130010/1‑148 (MQ=255) ggCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGGCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcagca < 2:84318/149‑1 (MQ=255) ggCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcagca < 5:56027/149‑1 (MQ=255) gCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcag > 5:109278/1‑146 (MQ=255) gCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcag > 6:205362/1‑142 (MQ=255) gCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACcag < 5:205362/142‑1 (MQ=255) gCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTg > 5:205699/1‑149 (MQ=255) cGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCAcgg < 4:194306/149‑1 (MQ=255) ggCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCAcggc < 6:166983/149‑1 (MQ=255) aaCGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgag < 6:43502/149‑1 (MQ=255) cGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTAGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgagga < 4:42067/149‑1 (MQ=255) cGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTAGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgagga < 4:38017/149‑1 (MQ=255) gggT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgaggag < 6:205699/149‑1 (MQ=255) ggT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgaggag > 4:38631/1‑148 (MQ=255) gCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGTGAGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGa > 1:218423/1‑149 (MQ=255) cAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGAcgg > 3:64260/1‑149 (MQ=255) tGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGc < 3:114669/148‑1 (MQ=255) gACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTCGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCCATGAACCACCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCg > 2:138886/1‑148 (MQ=255) aacaGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCgaaga < 7:137068/149‑1 (MQ=255) cAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCg < 5:167127/149‑1 (MQ=255) aaCTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgaggag < 4:252902/119‑1 (MQ=255) aaCTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCgaggag > 3:252902/1‑119 (MQ=255) aaCTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCg > 5:92495/1‑148 (MQ=255) aCAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAGTCAACCGCAGCGtgat > 5:37958/1‑148 (MQ=255) cagcag‑CGT‑AGAGGTGACCCGCCGCGCCGGAAGCTCGCTTGAAACCATCAGCCGCAGGGTGTCGTCTATGCAGGCGATGAACCAGCAGATTGCCAGGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGtgatga < 1:138886/149‑1 (MQ=38) cagcag‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGtgatga < 3:130010/149‑1 (MQ=255) agcag‑cGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGaa > 4:137292/1‑149 (MQ=255) gCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGata < 2:32832/149‑1 (MQ=255) gACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGAAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATAttt > 6:231933/1‑149 (MQ=255) aCCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTc > 5:45670/1‑149 (MQ=255) cccGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTcc < 5:231933/149‑1 (MQ=255) cccGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTcc < 4:64260/149‑1 (MQ=255) cgccgcgccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGa < 6:109278/149‑1 (MQ=255) gccgcgccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGAc > 3:43109/1‑149 (MQ=255) gccgcgccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGAc < 6:92495/149‑1 (MQ=255) cgcgccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCa < 8:125719/149‑1 (MQ=255) cgccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAGTCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGa < 6:37958/149‑1 (MQ=255) ccgGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGAcc > 2:6152/1‑149 (MQ=255) ggCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGACCTc < 4:43109/149‑1 (MQ=255) gCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGACCTcg < 2:218423/149‑1 (MQ=255) cTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAAtgt < 4:186558/120‑1 (MQ=255) cTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAAtgt > 3:186558/1‑120 (MQ=255) | CCCAGCGCACACAGCAGTCGACCGAAGAGATCGAAGAGTTGATTGCCGGCCTGCAAAGCGGCACCCAACGGGT‑GGCCAGCGTGATGGACAACAGCCGCCAACTGACCGACAGCAG‑CGT‑AGAGCTGACCCGCCGCGCCGGCAGCTCGCTGGAAACCATCACCCGCACGGTGTCGTCGATCCAGGCGATGAACCAGCAGATTGCCACGGCGGCCGAGGAGCAGACGGCGGTGGCCGAAGAAATCAACCGCAGCGTGATGAATGTGCGTGATATTTCCGACCAGACCTCG > NC_002947/5718842‑5719128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |