Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 432,019 C→T 8.3% E99K (GAG→AAG)  PP_0354 ← CBS domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947432,0190CT 8.3% 137.9 / 2.1 48E99K (GAG→AAG) PP_0354CBS domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (24/20);  new base T (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAG  >  NC_002947/431875‑432161
                                                                                                                                                 |                                                                                                                                              
gggCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCg                                                                                                                                            >  4:21751/1‑149 (MQ=255)
    aaacacccGAAACACATCCGACATCGAGAACACGCGGAAGAATGCGGCCTTGTTCACTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCACGGCTGCCAGCGGGGAGTGCTTCCCGCTgg                                                                                                                                        <  6:69291/144‑1 (MQ=255)
      aaTCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGGAGTGCTTCCCGCTggca                                                                                                                                      <  1:5422/149‑3 (MQ=255)
      aaTCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTg                                                                                                                                      <  2:164187/149‑1 (MQ=255)
            gAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGt                                                                                                                                       >  6:227961/1‑142 (MQ=255)
            gAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGt                                                                                                                                       <  5:227961/142‑1 (MQ=255)
                   aTCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTgg                                                                                                                         >  4:230326/1‑149 (MQ=255)
                    tCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCTGACCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTgg                                                                                                                         >  1:87723/1‑149 (MQ=255)
                      aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAAc                                                                                                                             >  1:213271/1‑142 (MQ=255)
                      aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAAc                                                                                                                             <  2:213271/142‑1 (MQ=255)
                      aGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGttt                                                                                                                      <  7:95341/149‑1 (MQ=255)
                       gACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTc                                                                                                                     >  2:150340/1‑149 (MQ=255)
                           tCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGaca                                                                                                                 >  7:252868/1‑149 (MQ=255)
                            cGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGCTCTCGACCGTGGTTTCGacac                                                                                                                >  8:56058/1‑149 (MQ=255)
                              agaACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACAccc                                                                                                              <  3:21751/149‑1 (MQ=255)
                                     gcgGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTgg                                                                                                       >  2:12653/1‑149 (MQ=255)
                                             aTGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGc                                                                                               <  5:297898/149‑1 (MQ=255)
                                                 ggCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGgc                                                                                           >  7:21332/1‑149 (MQ=255)
                                                       gTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTc                                                                                       <  3:230326/147‑1 (MQ=255)
                                                            gTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAAcc                                                                                <  4:67850/149‑1 (MQ=255)
                                                                      gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGc                                                                                                      <  6:228892/117‑1 (MQ=255)
                                                                      gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGc                                                                                                      >  5:228892/1‑117 (MQ=255)
                                                                      gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCt                                                                      <  8:21332/149‑1 (MQ=255)
                                                                      gAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCt                                                                      >  4:88657/1‑149 (MQ=255)
                                                                       aaGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGcc                                                                        <  1:296897/146‑1 (MQ=255)
                                                                       aaGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGcc                                                                        >  2:296897/1‑146 (MQ=255)
                                                                            gtCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGa                                                                >  5:30628/1‑149 (MQ=255)
                                                                             tCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGAc                                                               >  1:286584/1‑149 (MQ=255)
                                                                                  ccccGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATg                                                           >  5:20503/1‑149 (MQ=255)
                                                                                    ccGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTa                                                         <  2:286584/149‑1 (MQ=255)
                                                                                       gCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAg                                                        >  3:251516/1‑147 (MQ=255)
                                                                                           ggTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGc                                                  <  8:252868/149‑1 (MQ=255)
                                                                                                tCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGAc                                             >  5:23073/1‑149 (MQ=255)
                                                                                                  ggTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGAcc                                            <  6:20503/148‑1 (MQ=255)
                                                                                                    tGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGc                                         <  7:56058/149‑1 (MQ=255)
                                                                                                     gCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGcc                                        >  2:16016/1‑149 (MQ=255)
                                                                                                      cGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                       <  6:30628/149‑1 (MQ=255)
                                                                                                      cGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGccc                                       <  3:88657/149‑1 (MQ=255)
                                                                                                            gCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTACTTGACGATGTAGAAGTGCTCGACCGGCCCGTCgg                                  >  5:41031/1‑148 (MQ=255)
                                                                                                            gCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTACTTGACGATGTAGAAGTGCTCGACCGGCCCGTCgg                                  <  6:41031/148‑1 (MQ=255)
                                                                                                                      ccAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGAc                        >  8:22965/1‑148 (MQ=255)
                                                                                                                            agatcaccAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCtt                 >  6:33843/7‑149 (MQ=255)
                                                                                                                                 accAGCGGGGAGTGCTTCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCAcc            >  4:167421/2‑149 (MQ=255)
                                                                                                                                         gAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCg     >  3:161138/1‑148 (MQ=255)
                                                                                                                                           aGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCg     >  5:172742/1‑146 (MQ=255)
                                                                                                                                           aGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAg  <  4:161138/149‑1 (MQ=255)
                                                                                                                                             cACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGa                         <  2:187734/124‑1 (MQ=255)
                                                                                                                                             cACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGa                         >  1:187734/1‑124 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GGGCAAAATCCCGAAACACATCAGACATCGAGAACACGCGGATGAATGCGGCCTTGTTCAGTTGCAGGCAGAAGGTGTCT‑TCCCCGGCCAGGTGCTCGGTGCGGGTTGCCCGCTCACCCAGCAGGGCTGCCAGCGGGAAGCACTCCCCGCTGGTGATCTCGAACGTGGTTTCGACACCCGGTTTGGCCAGGTGCTGGCGCTCGCCAACCACGCGGCCCTGCTTGACGATGTAGAAGTGCTCGACCGGCCCGTCGGCAGGTTTGACGATGCTTTCACCGCAGGCGTAG  >  NC_002947/431875‑432161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑