Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,728,840 A→G 9.6% intergenic (‑203/+121) opgG ← / ← dtd glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,728,8400AG 9.6% 168.3 / 6.6 62intergenic (‑203/+121)opgG/dtdglucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base G (4/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATG  >  NC_002947/5728694‑5728980
                                                                                                                                                  |                                                                                                                                            
tCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTAc                                                                                                                                             >  7:85623/1‑148 (MQ=255)
  aCGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTACCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGaa                                                                                                                                          >  7:189849/1‑149 (MQ=255)
      gCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACg                                                                                                                                        >  1:213372/1‑147 (MQ=255)
      gCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACg                                                                                                                                        >  4:145083/1‑147 (MQ=255)
       cAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAg                                                                                                                                     >  1:55511/1‑149 (MQ=255)
          cccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGTCAATGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTc                                                                                                                                  >  3:262542/1‑149 (MQ=255)
           ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGTAACGGACCTACGAACGCAGGTcc                                                                                                                                 <  6:278963/149‑1 (MQ=255)
            cTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCa                                                                                                                                >  4:71192/1‑149 (MQ=255)
               gCCGAAAACAGATCATGAACCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGGTCGGCCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAAAGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTccgt                                                                                                                               <  4:12812/147‑3 (MQ=255)
               gCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg                                                                                                                              >  3:292061/1‑148 (MQ=255)
                ccGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg                                                                                                                              >  8:70258/1‑147 (MQ=255)
                  gAAAACAGATGATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGTCAATGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTccgtttcac                                                                                                                          <  4:262542/149‑8 (MQ=255)
                       cAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGcgcgcg                                                                                                                     <  6:261215/149‑1 (MQ=255)
                        aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa                                                                                                                    <  4:292061/149‑1 (MQ=255)
                               gAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                               >  1:200102/1‑147 (MQ=255)
                                  aCCAACGGCCAGCGACAAATGCCGGGTTGGCGATCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcagca                                                                                                          <  8:189849/149‑1 (MQ=255)
                                               gACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACaa                                                                                             >  7:8892/1‑149 (MQ=255)
                                               gACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACaa                                                                                             <  2:213372/149‑1 (MQ=255)
                                                    aTGCCGGGTTGGCGAGCACTGTTTGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCAt                                                                                        <  6:286455/149‑1 (MQ=255)
                                                    aTGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGCATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCAt                                                                                        <  5:100053/149‑1 (MQ=255)
                                                     tGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                               >  2:49137/1‑125 (MQ=255)
                                                     tGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc                                                                                                               <  1:49137/125‑1 (MQ=255)
                                                        cGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAgg                                                                                    <  2:200102/149‑1 (MQ=255)
                                                           gTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTg                                                                                 <  7:49672/149‑1 (MQ=255)
                                                             tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCAt                                                                                                                               <  3:262305/101‑1 (MQ=255)
                                                             tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCAt                                                                                                                               >  4:262305/1‑101 (MQ=255)
                                                                             gACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTa                                                               <  5:109333/149‑1 (MQ=255)
                                                                              aCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc                                                              <  8:85623/149‑1 (MQ=255)
                                                                                     gagGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAAccc                                                       <  2:55511/149‑1 (MQ=255)
                                                                                        ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                    <  7:70258/149‑1 (MQ=255)
                                                                                        ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt                                                    <  8:220095/149‑1 (MQ=255)
                                                                                          cGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCt                                                                                  <  8:97609/117‑1 (MQ=255)
                                                                                           gTAATGATTCCCCGAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGtt                                                 >  7:74930/1‑149 (MQ=255)
                                                                                            tAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGttt                                                >  5:260015/1‑149 (MQ=255)
                                                                                                gATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc                                                              >  4:178170/1‑131 (MQ=255)
                                                                                                 aTTCCCCAAACGGATCCAGACAATGCTCGAGTCATAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAt                                                                       <  7:187253/121‑1 (MQ=255)
                                                                                                 aTTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGCCTGGAGCAACTAt                                                                       >  8:187253/1‑121 (MQ=255)
                                                                                                  ttCCCCAACCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt                                          <  7:116807/149‑1 (MQ=255)
                                                                                                  ttCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt                                          >  8:229373/1‑149 (MQ=255)
                                                                                                     cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTGCTGTGa                                        >  8:113138/1‑148 (MQ=255)
                                                                                                     cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa                                       <  3:145083/149‑1 (MQ=255)
                                                                                                     cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa                                        >  4:198579/1‑148 (MQ=255)
                                                                                                     cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa                                        >  5:38193/1‑148 (MQ=255)
                                                                                                        aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                 <  6:155593/120‑1 (MQ=255)
                                                                                                        aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg                                                                 >  5:155593/1‑120 (MQ=255)
                                                                                                         aaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaac                                   >  3:277202/1‑149 (MQ=255)
                                                                                                                  aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                          <  8:74930/149‑1 (MQ=255)
                                                                                                                  aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt                          <  3:71192/149‑1 (MQ=255)
                                                                                                                      aaTGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGAcc                      >  3:818/1‑149 (MQ=255)
                                                                                                                          cTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCa                   <  6:285276/148‑1 (MQ=255)
                                                                                                                           tCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCata                 >  5:224234/1‑149 (MQ=255)
                                                                                                                             gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt               >  3:86552/1‑149 (MQ=255)
                                                                                                                              aGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAttt              <  7:229373/149‑1 (MQ=255)
                                                                                                                                  agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTaa          <  3:198579/149‑1 (MQ=255)
                                                                                                                                  agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTa           >  7:109996/1‑148 (MQ=255)
                                                                                                                                  agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTa           <  5:38778/148‑1 (MQ=255)
                                                                                                                                    agTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACa        >  4:290194/1‑149 (MQ=255)
                                                                                                                                      ctgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACAt       >  1:175418/7‑148 (MQ=255)
                                                                                                                                       tgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATa      >  3:254966/6‑148 (MQ=255)
                                                                                                                                       tgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATa      >  1:127631/6‑148 (MQ=255)
                                                                                                                                         aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt   <  6:260015/149‑1 (MQ=255)
                                                                                                                                          aCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATg  >  2:305428/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TCACGAGCAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATG  >  NC_002947/5728694‑5728980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑