| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,728,847 | C→T | 10.1% | intergenic (‑210/+114) | opgG ← / ← dtd | glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,728,847 | 0 | C | T | 10.1% | 161.3 / 7.0 | 59 | intergenic (‑210/+114) | opgG/dtd | glucans biosynthesis protein G/D‑tyrosyl‑tRNA(Tyr) deacylase |
| Reads supporting (aligned to +/- strand): ref base C (24/29); new base T (4/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGGCCATCA > NC_002947/5728701‑5728994 | cAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAg > 1:55511/1‑149 (MQ=255) cccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGTCAATGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTc > 3:262542/1‑149 (MQ=255) ccTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGTAACGGACCTACGAACGCAGGTcc < 6:278963/149‑1 (MQ=255) cTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCa > 4:71192/1‑149 (MQ=255) gCCGAAAACAGATCATGAACCCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGGTCGGCCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAAAGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTccgt < 4:12812/147‑3 (MQ=255) gCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg > 3:292061/1‑148 (MQ=255) ccGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATg > 8:70258/1‑147 (MQ=255) gAAAACAGATGATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGTCAATGCTCGAGTCAGAGTGAAACGGACCTGCGTTCGTAGGTccgtttcac < 4:262542/149‑8 (MQ=255) cAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGcgcgcg < 6:261215/149‑1 (MQ=255) aGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGa < 4:292061/149‑1 (MQ=255) gAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc > 1:200102/1‑147 (MQ=255) aCCAACGGCCAGCGACAAATGCCGGGTTGGCGATCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCcagca < 8:189849/149‑1 (MQ=255) gACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACaa < 2:213372/149‑1 (MQ=255) gACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACaa > 7:8892/1‑149 (MQ=255) aTGCCGGGTTGGCGAGCACTGTTTGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCAt < 6:286455/149‑1 (MQ=255) aTGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGCATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCAt < 5:100053/149‑1 (MQ=255) tGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc < 1:49137/125‑1 (MQ=255) tGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATc > 2:49137/1‑125 (MQ=255) cGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAgg < 2:200102/149‑1 (MQ=255) gTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTg < 7:49672/149‑1 (MQ=255) tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCAt > 4:262305/1‑101 (MQ=255) tGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCAt < 3:262305/101‑1 (MQ=255) gACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTa < 5:109333/149‑1 (MQ=255) aCCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc < 8:85623/149‑1 (MQ=255) gagGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAAccc < 2:55511/149‑1 (MQ=255) ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt < 7:70258/149‑1 (MQ=255) ggCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCtt < 8:220095/149‑1 (MQ=255) cGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCt < 8:97609/117‑1 (MQ=255) gTAATGATTCCCCGAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGtt > 7:74930/1‑149 (MQ=255) tAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGttt > 5:260015/1‑149 (MQ=255) gATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTAc > 4:178170/1‑131 (MQ=255) aTTCCCCAAACGGATCCAGACAATGCTCGAGTCATAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTAt < 7:187253/121‑1 (MQ=255) aTTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGCCTGGAGCAACTAt > 8:187253/1‑121 (MQ=255) ttCCCCAACCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt < 7:116807/149‑1 (MQ=255) ttCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCtgt > 8:229373/1‑149 (MQ=255) cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTGCTGTGa > 8:113138/1‑148 (MQ=255) cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaa < 3:145083/149‑1 (MQ=255) cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa > 4:198579/1‑148 (MQ=255) cccAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGa > 5:38193/1‑148 (MQ=255) aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg > 5:155593/1‑120 (MQ=255) aaaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCg < 6:155593/120‑1 (MQ=255) aaCGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGaacaac > 3:277202/1‑149 (MQ=255) aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt < 8:74930/149‑1 (MQ=255) aGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGtttttt < 3:71192/149‑1 (MQ=255) aaTGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGAcc > 3:818/1‑149 (MQ=255) cTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCa < 6:285276/148‑1 (MQ=255) tCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCata > 5:224234/1‑149 (MQ=255) gAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAtt > 3:86552/1‑149 (MQ=255) aGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATAttt < 7:229373/149‑1 (MQ=255) agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTaa < 3:198579/149‑1 (MQ=255) agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTa < 5:38778/148‑1 (MQ=255) agagTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTa > 7:109996/1‑148 (MQ=255) agTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACa > 4:290194/1‑149 (MQ=255) ctgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACAt > 1:175418/7‑148 (MQ=255) tgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATa > 3:254966/6‑148 (MQ=255) tgcatggACCTGCGTTCGTAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATa > 1:127631/6‑148 (MQ=255) aaCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAAt < 6:260015/149‑1 (MQ=255) aCGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATg > 2:305428/1‑149 (MQ=255) gCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGGCcatca < 6:224234/149‑1 (MQ=255) | CAACCCTCGCCGAAAACAGATCATGAAACCAACGGCCAGCGACAAATGCCGGGTTGGCGAGCACTGTTCGGACCCCGAGAGGGCGTAATGATTCCCCAAACGGATCCAGACAATGCTCGAGTCAGAGTGAAACGGACCTACGAACGCAGGTCCATGCAGCGCGCGATTATCCAGCAGGTTGCGTGACAACGCATCAGGCTGGAGCAACTATTTATCGTACAAAACCCCTTGTTTTCCTGTGAACAACGGGTTTTTTGACCTCATATTTGTAACATAAATGTTACCGGGCCATCA > NC_002947/5728701‑5728994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |