Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,740,467	T→G	36.7%	intergenic (+32/+39)	hutF → / ← PP_5037	formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,740,467	0	T	G	36.7%	15.9 / 34.4	41	intergenic (+32/+39)	hutF/PP_5037	formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (13/13);  new base G (6/9);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.75e-01

GCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGG  >  NC_002947/5740320‑5740613                                                                                                                                                    |                                                                                                                                                    gCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGt                                                                                                                                                     <  1:9234/148‑1 (MQ=255)  ccAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGc                                                                                                                                                   <  7:2594/149‑1 (MQ=255)     ggTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGcttg                                                                                                                                                <  7:36121/149‑4 (MQ=255)      gTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATCGCGTGCaacc                                                                                                                                               >  8:66333/1‑147 (MQ=255)            gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑a                                                                                                                                        <  6:263849/149‑1 (MQ=39)             aTGTGTTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAATGCGGGCGGGGGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTGGCGTTGCAGCGCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                       <  8:184392/149‑1 (MQ=255)             aTGTGATGGTGTATGGGCAATGGGTGGTGCGCCAGGGGCGGCAGGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                       <  2:112370/149‑1 (MQ=38)                   tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTg                                                                                                                                 <  7:137894/149‑1 (MQ=38)                     gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGGTTGGGCCCATGGCGTGGGCGGCCGCG‑CAGGGTTgg                                                                                                                                >  3:23226/1‑147 (MQ=255)                     gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTCCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTgca                                                                                                                               <  1:203351/149‑1 (MQ=255)                          tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCTTAGCGTGGAAGCCCGCG‑AAGGGTTgccacgc                                                                                                                           >  3:210640/1‑148 (MQ=255)                                        tgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCGTGGCGTGCAAGCCCGCG‑GAGGGTTGGACCGCCACCCCAAAAAACTc                                                                                                            >  1:9849/1‑149 (MQ=255)                                            ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                              <  6:304099/127‑1 (MQ=37)                                            ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                              >  5:304099/1‑127 (MQ=37)                                                 gcgggcATGCCCGGTATGTTGAACGCGGGGTGGCGTTTGAAATGGGTTTGCGCATGGTTTTTGGCTGTGTGTACCTGGGTGGTGTTGCTGCCTTTGGTGGCCAACCCCGTG‑ATGGGTTTCAACGCAACCCCAAAAAACTCAGTGCAttt                                                                                                   <  4:258301/146‑1 (MQ=255)                                                  gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                    >  8:258896/1‑115 (MQ=37)                                                  gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                    <  7:258896/115‑1 (MQ=37)                                                     gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCGTAGCGGGGAAGCCCGCG‑CAGGGTTGCAACGCCACCCCAAAAAACGCAGTGCCATTTTAc                                                                                               >  6:151195/1‑149 (MQ=255)                                                     gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑TAGGGTTGCAAAGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                                >  6:295247/1‑148 (MQ=255)                                                           ccAGGAAGATGAACGCGCGCGGGCTGTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                         <  4:210640/149‑1 (MQ=255)                                                                 aGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                   <  5:151195/149‑1 (MQ=255)                                                                                 gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATc                                                                   <  7:66333/149‑1 (MQ=255)                                                                                         cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCCTTTCAGCCCATAGCGGGCAATCCCCCG‑AACGGATGCAACGCAACCCCAAAAAACACACGTCAATTTTAATATTTTCTGATCGGCCCCCCCCCCCATCAAACGGcg                                                           >  8:173540/1‑148 (MQ=255)                                                                                                    gcCAGGTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCGGCC‑TAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcc                                                <  1:12012/149‑1 (MQ=255)                                                                                                    gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGGAGCCCATAGCGGGGCAGCCCGCGCACGGTTTGCAA‑GCAACCCCCAAAAAAAAAGTGCATTTTTTATATTTTGTGATAGGCCCCCCCCCACATAAAGAGGGGGGGGTGATAcc                                                >  2:78256/1‑149 (MQ=255)                                                                                                                    aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCT‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                <  4:23226/149‑1 (MQ=255)                                                                                                                      cttACGGGGTTTGCGTTGAATCCCTTCGTGTTAAAGCCCTCT‑ATTGTTTTAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                               <  4:24987/146‑1 (MQ=255)                                                                                                                        tACCGGGGTTGCGTGGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                            <  2:9849/149‑1 (MQ=255)                                                                                                                         aCCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgc                                                                         <  2:43820/103‑1 (MQ=255)                                                                                                                         aCCGGGGTTGCGTTGCAGCCCATCGCGTGCCAGCCCGCG‑AAGGGTTGGAACGCAACCCCAAAAAACTCAGTGGATTTTTTATATTTGCTGATCGGccgcccgc                                                                         >  1:43820/1‑103 (MQ=255)                                                                                                                            ggggTTGCGTTGCAGCCCTTCGCGTGCATGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                        <  7:173540/149‑1 (MQ=255)                                                                                                                            ggggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                        <  4:217484/149‑1 (MQ=255)                                                                                                                                        cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt            >  6:157745/1‑149 (MQ=255)                                                                                                                                        cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                            <  7:232746/117‑1 (MQ=255)                                                                                                                                        cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                            >  8:232746/1‑117 (MQ=255)                                                                                                                                         aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                     <  1:74080/121‑1 (MQ=38)                                                                                                                                         aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                     >  2:74080/3‑123 (MQ=38)                                                                                                                                         aGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc           >  1:244675/1‑149 (MQ=255)                                                                                                                                          gCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGGGTCATACCCCTGCTGCCGCGCCCGGGCCAGCAGGGTCTCACGCAcc          >  8:60650/1‑149 (MQ=255)                                                                                                                                             cTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGTCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg       >  6:22610/1‑149 (MQ=255)                                                                                                                                                 gcgTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg   >  5:155177/1‑149 (MQ=255)                                                                                                                                                   gTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg  >  2:768/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCCAGGTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGG  >  NC_002947/5740320‑5740613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑