Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,470 A→T 27.6% intergenic (+35/+36) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4700AT27.6% 44.0 / 21.8 39intergenic (+35/+36)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (13/13);  new base T (3/7);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01

GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGT  >  NC_002947/5740325‑5740617
                                                                                                                                                 |                                                                                                                                                    
gTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCATCGCGTGCaacc                                                                                                                                                   >  8:66333/1‑147 (MQ=255)
      gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑a                                                                                                                                            <  6:263849/149‑1 (MQ=39)
       aTGTGTTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAATGCGGGCGGGGGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTGGCGTTGCAGCGCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                           <  8:184392/149‑1 (MQ=255)
       aTGTGATGGTGTATGGGCAATGGGTGGTGCGCCAGGGGCGGCAGGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                           <  2:112370/149‑1 (MQ=38)
             tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTg                                                                                                                                     <  7:137894/149‑1 (MQ=38)
               gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGGTTGGGCCCATGGCGTGGGCGGCCGCG‑CAGGGTTgg                                                                                                                                    >  3:23226/1‑147 (MQ=255)
               gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTCCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTgca                                                                                                                                   <  1:203351/149‑1 (MQ=255)
                    tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCTTAGCGTGGAAGCCCGCG‑AAGGGTTgccacgc                                                                                                                               >  3:210640/1‑148 (MQ=255)
                                  tgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCGTGGCGTGCAAGCCCGCG‑GAGGGTTGGACCGCCACCCCAAAAAACTc                                                                                                                >  1:9849/1‑149 (MQ=255)
                                      ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                                  <  6:304099/127‑1 (MQ=37)
                                      ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                                  >  5:304099/1‑127 (MQ=37)
                                           gcgggcATGCCCGGTATGTTGAACGCGGGGTGGCGTTTGAAATGGGTTTGCGCATGGTTTTTGGCTGTGTGTACCTGGGTGGTGTTGCTGCCTTTGGTGGCCAACCCCGTG‑ATGGGTTTCAACGCAACCCCAAAAAACTCAGTGCAttt                                                                                                       <  4:258301/146‑1 (MQ=255)
                                            gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                        >  8:258896/1‑115 (MQ=37)
                                            gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                        <  7:258896/115‑1 (MQ=37)
                                               gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCGTAGCGGGGAAGCCCGCG‑CAGGGTTGCAACGCCACCCCAAAAAACGCAGTGCCATTTTAc                                                                                                   >  6:151195/1‑149 (MQ=255)
                                               gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑TAGGGTTGCAAAGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                                    >  6:295247/1‑148 (MQ=255)
                                                     ccAGGAAGATGAACGCGCGCGGGCTGTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                             <  4:210640/149‑1 (MQ=255)
                                                           aGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                       <  5:151195/149‑1 (MQ=255)
                                                                           gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATc                                                                       <  7:66333/149‑1 (MQ=255)
                                                                                   cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCCTTTCAGCCCATAGCGGGCAATCCCCCG‑AACGGATGCAACGCAACCCCAAAAAACACACGTCAATTTTAATATTTTCTGATCGGCCCCCCCCCCCATCAAACGGcg                                                               >  8:173540/1‑148 (MQ=255)
                                                                                              gcCAGGTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCGGCC‑TAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcc                                                    <  1:12012/149‑1 (MQ=255)
                                                                                              gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGGAGCCCATAGCGGGGCAGCCCGCGCACGGTTTGCAA‑GCAACCCCCAAAAAAAAAGTGCATTTTTTATATTTTGTGATAGGCCCCCCCCCACATAAAGAGGGGGGGGTGATAcc                                                    >  2:78256/1‑149 (MQ=255)
                                                                                                              aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCT‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                    <  4:23226/149‑1 (MQ=255)
                                                                                                                cttACGGGGTTTGCGTTGAATCCCTTCGTGTTAAAGCCCTCT‑ATTGTTTTAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                   <  4:24987/146‑1 (MQ=255)
                                                                                                                  tACCGGGGTTGCGTGGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                                <  2:9849/149‑1 (MQ=255)
                                                                                                                   aCCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgc                                                                             <  2:43820/103‑1 (MQ=255)
                                                                                                                   aCCGGGGTTGCGTTGCAGCCCATCGCGTGCCAGCCCGCG‑AAGGGTTGGAACGCAACCCCAAAAAACTCAGTGGATTTTTTATATTTGCTGATCGGccgcccgc                                                                             >  1:43820/1‑103 (MQ=255)
                                                                                                                      ggggTTGCGTTGCAGCCCTTCGCGTGCATGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                            <  7:173540/149‑1 (MQ=255)
                                                                                                                      ggggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                            <  4:217484/149‑1 (MQ=255)
                                                                                                                                  cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt                >  6:157745/1‑149 (MQ=255)
                                                                                                                                  cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                                <  7:232746/117‑1 (MQ=255)
                                                                                                                                  cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                                >  8:232746/1‑117 (MQ=255)
                                                                                                                                   aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                         <  1:74080/121‑1 (MQ=38)
                                                                                                                                   aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                         >  2:74080/3‑123 (MQ=38)
                                                                                                                                   aGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc               >  1:244675/1‑149 (MQ=255)
                                                                                                                                    gCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGGGTCATACCCCTGCTGCCGCGCCCGGGCCAGCAGGGTCTCACGCAcc              >  8:60650/1‑149 (MQ=255)
                                                                                                                                       cTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGTCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg           >  6:22610/1‑149 (MQ=255)
                                                                                                                                           gcgTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg       >  5:155177/1‑149 (MQ=255)
                                                                                                                                             gTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg      >  2:768/1‑148 (MQ=255)
                                                                                                                                                cAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgt  >  2:181038/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTGCGCGATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGT  >  NC_002947/5740325‑5740617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑