Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	441,599	G→T	14.5%	L160L (CTG→CTT)	bioF →	8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	441,599	0	G	T	14.5%	100.7 / 8.7	41	L160L (CTG→CTT)	bioF	8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base G (19/16);  new base T (4/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

TGGGCGCCATCACCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCC  >  NC_002947/441451‑441744                                                                                                                                                       |                                                                                                                                                  tGGGCGCCATCACCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑Tg                                                                                                                                                   <  6:181436/149‑1 (MQ=255)  gggCGCCATCACCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑Tg                                                                                                                                                   >  8:140148/1‑148 (MQ=255)        cATCACCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAgg                                                                                                                                            <  3:109270/149‑1 (MQ=255)            aCCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGt                                                                                                                                        <  2:80780/149‑1 (MQ=255)                         ggCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCtg                                                                                                                           <  1:17374/149‑1 (MQ=255)                              ggCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTggtggt                                                                                                                      >  6:18658/1‑149 (MQ=255)                                             cAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCCATGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCgtgt                                                                                                        <  1:62957/149‑1 (MQ=255)                                             cAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCgtgt                                                                                                        <  3:253697/148‑1 (MQ=255)                                                            cATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGTGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGa                                                                                        >  4:235224/1‑149 (MQ=255)                                                                 cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGAc                                                                                       <  6:210934/145‑1 (MQ=255)                                                                 cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGAc                                                                                       >  5:210934/1‑145 (MQ=255)                                                                 cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTgg                                                                                   >  5:210956/1‑149 (MQ=255)                                                                  tCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCgg                                                                                                                                     >  3:154590/1‑98 (MQ=255)                                                                  tCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCgg                                                                                                                                     <  4:154590/98‑1 (MQ=255)                                                                    gTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCTACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGcc                                                                                 <  7:140148/148‑1 (MQ=255)                                                                      tGCTGGATGGCGGGTTGCTCAGAGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCAAGCCT‑GGCCAGCCGCC‑TTGACAAGGCGGTCGGCAATACACTGCTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGAc                                                                              >  5:215821/1‑149 (MQ=255)                                                                       gCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGAc                                                                              <  1:261074/148‑1 (MQ=255)                                                                          ggATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGc                                                                          >  2:111523/1‑149 (MQ=255)                                                                                cGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGgcgc                                                                    <  5:18658/149‑1 (MQ=255)                                                                                   gtcgCTCAGTGGCGCCCGCTTCTACCGCTACCTGCACAACCACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGCACGGTGACCTGGCCGACCTGCCGGCGCTgg                                                                 <  6:253230/146‑1 (MQ=255)                                                                                          aGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCTGGGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATg                                                           >  1:71991/1‑149 (MQ=255)                                                                                              gtgCCCGGGTCAACCGCTACCTGCACTACGAGCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACTGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGc                                                       <  6:215821/148‑1 (MQ=255)                                                                                              gtgCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGcc                                                      >  2:30038/1‑149 (MQ=255)                                                                                                 cccGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGt                                                   >  8:156180/1‑149 (MQ=255)                                                                                                  ccGCTTCAACTGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTg                                                  >  2:307858/1‑149 (MQ=255)                                                                                                     cTTCAACCGCTACCTGCACACCGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGccc                                               >  5:54713/1‑149 (MQ=255)                                                                                                          aCCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGt                                          >  8:14859/1‑149 (MQ=255)                                                                                                                  cTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCt                                  >  6:254368/1‑149 (MQ=255)                                                                                                                     cacaACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGa                                <  6:210956/148‑1 (MQ=255)                                                                                                                         ggcggctggCCAGGCT‑GGCCGGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTc                           >  2:259265/8‑149 (MQ=255)                                                                                                                                       cT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg             >  3:208603/1‑149 (MQ=255)                                                                                                                                       at‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg             <  1:258928/148‑1 (MQ=255)                                                                                                                                        t‑GGCCAGCAGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATTGACGGTGACCTGGCCGACATGCCGGCGCTGGCCAATGTCGCCCGACCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg             >  2:265083/1‑148 (MQ=255)                                                                                                                                         ttgccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGc            >  6:57015/8‑149 (MQ=255)                                                                                                                                            ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGcc                                       >  2:144948/5‑119 (MQ=255)                                                                                                                                            ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGcc                                       <  1:144948/115‑1 (MQ=255)                                                                                                                                            ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGAt                               <  7:83426/123‑1 (MQ=255)                                                                                                                                            ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGAt                               >  8:83426/5‑127 (MQ=255)                                                                                                                                             cAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg        <  7:156180/149‑1 (MQ=255)                                                                                                                                             cAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg        >  8:286158/1‑149 (MQ=255)                                                                                                                                                  gcc‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAcc   >  6:184363/1‑149 (MQ=255)                                                                                                                                                   cc‑TGGACAAGGTGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAccc  <  3:235224/149‑1 (MQ=255)                                                                                                                                                   cc‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAccc  >  2:51607/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                  TGGGCGCCATCACCGCGCTGGTGGGGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCC  >  NC_002947/441451‑441744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑