Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,474 C→A 23.8% intergenic (+39/+32) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4740CA23.8% 73.4 / 23.6 42intergenic (+39/+32)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (18/14);  new base A (3/7);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01

GATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGG  >  NC_002947/5740331‑5740622
                                                                                                                                               |                                                                                                                                                     
gATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑a                                                                                                                                                 <  6:263849/149‑1 (MQ=39)
 aTGTGTTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAATGCGGGCGGGGGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTGGCGTTGCAGCGCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                                <  8:184392/149‑1 (MQ=255)
 aTGTGATGGTGTATGGGCAATGGGTGGTGCGCCAGGGGCGGCAGGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑aa                                                                                                                                                <  2:112370/149‑1 (MQ=38)
       tGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTg                                                                                                                                          <  7:137894/149‑1 (MQ=38)
         gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGGGTTGGGCCCATGGCGTGGGCGGCCGCG‑CAGGGTTgg                                                                                                                                         >  3:23226/1‑147 (MQ=255)
         gTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTCCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTgca                                                                                                                                        <  1:203351/149‑1 (MQ=255)
              tGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTCAGCCCTTAGCGTGGAAGCCCGCG‑AAGGGTTgccacgc                                                                                                                                    >  3:210640/1‑148 (MQ=255)
                            tgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCGTGGCGTGCAAGCCCGCG‑GAGGGTTGGACCGCCACCCCAAAAAACTc                                                                                                                     >  1:9849/1‑149 (MQ=255)
                                ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                                       >  5:304099/1‑127 (MQ=37)
                                ccAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAGGGCTgcaa                                                                                                                                       <  6:304099/127‑1 (MQ=37)
                                     gcgggcATGCCCGGTATGTTGAACGCGGGGTGGCGTTTGAAATGGGTTTGCGCATGGTTTTTGGCTGTGTGTACCTGGGTGGTGTTGCTGCCTTTGGTGGCCAACCCCGTG‑ATGGGTTTCAACGCAACCCCAAAAAACTCAGTGCAttt                                                                                                            <  4:258301/146‑1 (MQ=255)
                                      gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                             <  7:258896/115‑1 (MQ=37)
                                      gcggcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAACCCTTCGCGGGCTTGCACGCG‑AAggg                                                                                                                                             >  8:258896/1‑115 (MQ=37)
                                         gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTTGAGCCCGTAGCGGGGAAGCCCGCG‑CAGGGTTGCAACGCCACCCCAAAAAACGCAGTGCCATTTTAc                                                                                                        >  6:151195/1‑149 (MQ=255)
                                         gcATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑TAGGGTTGCAAAGCAACCCCAAAAAACTCAGTGCATTTTTa                                                                                                         >  6:295247/1‑148 (MQ=255)
                                               ccAGGAAGATGAACGCGCGCGGGCTGTTGCCAAGGGGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTg                                                                                                  <  4:210640/149‑1 (MQ=255)
                                                     aGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATc                                                                                            <  5:151195/149‑1 (MQ=255)
                                                                     gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATc                                                                            <  7:66333/149‑1 (MQ=255)
                                                                             cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCCTTTCAGCCCATAGCGGGCAATCCCCCG‑AACGGATGCAACGCAACCCCAAAAAACACACGTCAATTTTAATATTTTCTGATCGGCCCCCCCCCCCATCAAACGGcg                                                                    >  8:173540/1‑148 (MQ=255)
                                                                                        gcCAGGTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCGGCC‑TAGGTTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAcc                                                         <  1:12012/149‑1 (MQ=255)
                                                                                        gcCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGGAGCCCATAGCGGGGCAGCCCGCGCACGGTTTGCAA‑GCAACCCCCAAAAAAAAAGTGCATTTTTTATATTTTGTGATAGGCCCCCCCCCACATAAAGAGGGGGGGGTGATAcc                                                         >  2:78256/1‑149 (MQ=255)
                                                                                                        aGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCT‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCt                                         <  4:23226/149‑1 (MQ=255)
                                                                                                          cttACGGGGTTTGCGTTGAATCCCTTCGTGTTAAAGCCCTCT‑ATTGTTTTAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                        <  4:24987/146‑1 (MQ=255)
                                                                                                            tACCGGGGTTGCGTGGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAc                                     <  2:9849/149‑1 (MQ=255)
                                                                                                             aCCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgc                                                                                  <  2:43820/103‑1 (MQ=255)
                                                                                                             aCCGGGGTTGCGTTGCAGCCCATCGCGTGCCAGCCCGCG‑AAGGGTTGGAACGCAACCCCAAAAAACTCAGTGGATTTTTTATATTTGCTGATCGGccgcccgc                                                                                  >  1:43820/1‑103 (MQ=255)
                                                                                                                ggggTTGCGTTGCAGCCCTTCGCGTGCATGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                 <  7:173540/149‑1 (MQ=255)
                                                                                                                ggggTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcagca                                 <  4:217484/149‑1 (MQ=255)
                                                                                                                            cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCt                     >  6:157745/1‑149 (MQ=255)
                                                                                                                            cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                                     >  8:232746/1‑117 (MQ=255)
                                                                                                                            cAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTAACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctg                                                     <  7:232746/117‑1 (MQ=255)
                                                                                                                             aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                              <  1:74080/121‑1 (MQ=38)
                                                                                                                             aacccTTCGCGGGCTTGCACGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCcgc                                              >  2:74080/3‑123 (MQ=38)
                                                                                                                             aGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc                    >  1:244675/1‑149 (MQ=255)
                                                                                                                              gCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGGGTCATACCCCTGCTGCCGCGCCCGGGCCAGCAGGGTCTCACGCAcc                   >  8:60650/1‑149 (MQ=255)
                                                                                                                                 cTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGTCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg                >  6:22610/1‑149 (MQ=255)
                                                                                                                                     gcgTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCg            >  5:155177/1‑149 (MQ=255)
                                                                                                                                       gTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg           >  2:768/1‑148 (MQ=255)
                                                                                                                                          cAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgt       >  2:181038/1‑149 (MQ=255)
                                                                                                                                            aGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAgtgt     <  6:155177/149‑1 (MQ=255)
                                                                                                                                             gCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTc    >  6:283505/1‑149 (MQ=255)
                                                                                                                                             gCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTc    >  5:10874/1‑149 (MQ=255)
                                                                                                                                              cccGCT‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGAGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCg   <  6:8112/149‑1 (MQ=255)
                                                                                                                                               ccGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCgg  <  5:283505/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGCGTTGCAGCCCTTCGCGTGCAAGCCCGCG‑AAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAGTGTCGG  >  NC_002947/5740331‑5740622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑