Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,751,719 G→T 8.8% intergenic (‑157/‑314) thiI ← / → glnA tRNA sulfurtransferase/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,751,7190GT 8.8% 109.6 / 2.0 42intergenic (‑157/‑314)thiI/glnAtRNA sulfurtransferase/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base G (18/20);  new base T (2/2);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

CGCGCGCAGGGCCTGCCGAAAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGC  >  NC_002947/5751573‑5751866
                                                                                                                                                  |                                                                                                                                                   
cgcgcgcAGGGCCTGCCGAAAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGcc                                                                                                                                                   >  8:268822/1‑149 (MQ=255)
           ccTGCCGAAAATGTGGCGCGCTAATTATATCGGAAATTGCTCAGGATTTTACCAACTTTTGATGAGAAGCTTTGAGATTATTGCATAGTCCAGGTTTTGCAGCGCATGTTCCGGCCCTTTCGCTGGTAAACCCGCTCCTACAGGCGGTg                                                                                                                                        <  6:45024/149‑1 (MQ=255)
               ccGAAAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGAATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTTAACCAGCGCCGACAGGAGGTGCa                                                                                                                                      >  8:112306/1‑147 (MQ=255)
                 gAAAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGc                                                                                                                                  >  6:243775/1‑149 (MQ=255)
                      tGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTa                                                                                                                             <  7:268822/149‑1 (MQ=255)
                         ggggCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAg                                                                                                                            >  1:63936/1‑147 (MQ=255)
                              gcgAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCggg                                                                                                                     <  6:159057/149‑1 (MQ=255)
                                gAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAAACCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGtt                                                                                                                   >  1:298960/1‑149 (MQ=255)
                                      tatCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTAccc                                                                                                              <  2:179002/148‑1 (MQ=255)
                                      tatCGGAAATTGCTCAGAATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcg                                                                                                             <  7:112306/149‑1 (MQ=255)
                                        tCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCcgc                                                                                                            <  5:243775/148‑1 (MQ=255)
                                                  ctatggATTTTCCCAATTTTTGATCTTAAGCTTTGTTATAATTGCAGCGGCCCGGTTTTGCAGCGCCTGTTCCGGCCTTTTCGGGGTTAAACCGGCGCCTACTGGGGGTGAAATGCCGTTTGTTGGGGGTTTACCCGCGAAGAGGccc                                                                                                  <  2:298960/144‑1 (MQ=255)
                                                  cTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGccc                                                                                                  >  7:175010/1‑148 (MQ=255)
                                                  cTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGccc                                                                                                  >  6:8855/1‑148 (MQ=255)
                                                   tCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCaaa                                                                                                                                    <  3:276236/113‑1 (MQ=255)
                                                   tCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCaaa                                                                                                                                    >  4:276236/1‑113 (MQ=255)
                                                     aGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGccc                                                                                                                                <  6:16914/115‑1 (MQ=255)
                                                     aGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGccc                                                                                                                                >  5:16914/1‑115 (MQ=255)
                                                     aGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCggg                                                                                                                     <  8:139434/126‑1 (MQ=255)
                                                     aGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCggg                                                                                                                     >  7:139434/1‑126 (MQ=255)
                                                           tGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTg                                                                                         <  2:257825/148‑1 (MQ=255)
                                                                       gATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTcac                                                                            >  2:167518/1‑149 (MQ=255)
                                                                            gAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTcacgccac                                                                       <  1:47446/149‑1 (MQ=255)
                                                                                           aTGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGc                                                        <  1:167518/149‑1 (MQ=255)
                                                                                                gagGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGt                                                          >  8:213115/1‑142 (MQ=255)
                                                                                                gagGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGt                                                          <  7:213115/142‑1 (MQ=255)
                                                                                                   gCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAAACCCCGCCGAAAAGCGGTGCCAAGCCCGTAGGAGCGGGGTTTACCGCCAAGAGGCCCGAGAAACAGGCACAACCCACACCCCACCCCCCCAAATGGTGGGACACGa                                                 >  4:153006/1‑148 (MQ=255)
                                                                                                     cAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCAcacca                                                                                 <  3:42597/114‑1 (MQ=255)
                                                                                                     cAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCAcacca                                                                                 >  4:42597/1‑114 (MQ=255)
                                                                                                           ttGCAGCGCCTGTTCCGGCCCTTTCGCGGTTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCaaaaaa                                         <  2:63936/148‑1 (MQ=255)
                                                                                                              cAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCa                                     <  5:8855/149‑1 (MQ=255)
                                                                                                              cAGCGCCTGTTCCGGCACTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCa                                     <  4:159070/149‑1 (MQ=255)
                                                                                                                         ccGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGt                           <  8:175010/148‑1 (MQ=255)
                                                                                                                          cGGCCCTTTCGCGGGTAAACCCGCGCCTTCAGGCGGTGCAAAGCCCGTAGGAGCGGGGTTACCCGCGAAGGGGGCCTAGAACCTGGCACACCCCTCACGCCACGCCCCCGATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGc                         >  5:182777/1‑149 (MQ=255)
                                                                                                                                cttCGCGGGTAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACttt                    >  4:141777/2‑148 (MQ=25)
                                                                                                                                        gTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACtt                     <  4:148286/139‑1 (MQ=255)
                                                                                                                                        gTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACtt                     >  3:148286/1‑139 (MQ=255)
                                                                                                                                         tAAACCCGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTaa          <  3:141777/149‑1 (MQ=37)
                                                                                                                                           aaCCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCacac                                                                                   >  6:253993/1‑74 (MQ=255)
                                                                                                                                           aaCCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCacac                                                                                   <  5:253993/74‑1 (MQ=255)
                                                                                                                                              ccGCTCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTAATGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCaa     >  7:280208/1‑149 (MQ=37)
                                                                                                                                              ccGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCTCCCTATTGGTGCGACACGATCAAAAAACGAATCGTAAGGGTGCACTTTGACCCGCTAAGCCaa     >  5:143800/1‑149 (MQ=255)
                                                                                                                                               cgctCCTACGGGCTTTGCACCGCCTGTAGGCGCGGGTTTACCCGCGAA‑AGGGCC‑GGAA‑CAGGCACACCACTCACGCCACGCACCCTATTGGTGCGCCACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGc  >  3:203276/1‑148 (MQ=18)
                                                                                                                                                gcgcCTACTGGCGGTGAAAAGCCCTTAGGAGGGGGTTAACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACg   <  6:182777/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCGCGCAGGGCCTGCCGAAAATGAGGGGCGCGAATTATATCGGAAATTGCTCAGGATTTGACCAACTTTTGATCAGAAGCTTTGAGATAAATGCAGAGGCCAGGTTTTGCAGCGCCTGTTCCGGCCCTTTCGCGGGTAAACCCGCGCCTACAGGCGGTGCAAAGCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCTAGAACCTGGCACACCACTCACGCCACGCACCCTATTGGTGCGACACGATCAAAAAACGCATCGTAAGGGTGCACTTTCACCCGCTAACCCAACGC  >  NC_002947/5751573‑5751866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑