Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,761,198 G→A 8.3% V84M (GTG→ATG)  gpmI → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,761,1980GA 8.3% 168.0 / 4.5 60V84M (GTG→ATG) gpmI2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (31/24);  new base A (3/2);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

TGACCGCCTGTGCGCCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGG  >  NC_002947/5761053‑5761345
                                                                                                                                                 |                                                                                                                                                   
tGACCGCCTGTGCGCCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGa                                                                                                                                                  >  4:33862/1‑149 (MQ=255)
    cGCCTGTGCGCCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGAcc                                                                                                                                                >  8:134353/1‑147 (MQ=255)
      ccTGTGCGCCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAgg                                                                                                                                            <  6:118574/149‑1 (MQ=255)
              gccaccAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCcgc                                                                                                                                    >  2:197138/5‑149 (MQ=255)
              ccACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCcgc                                                                                                                                    >  7:133957/1‑149 (MQ=255)
                aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGa                                                                                                                                  >  7:276480/1‑149 (MQ=255)
                      ccGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGc                                                                                                                              >  4:117812/1‑147 (MQ=255)
                             gCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttctt                                                                                                                      <  7:134353/148‑1 (MQ=255)
                                       cGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCgg                                                                                                           <  6:98095/149‑1 (MQ=255)
                                       cGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCgg                                                                                                           <  7:3872/149‑1 (MQ=255)
                                          cTCGGGCATGGATGTCGGCCTGCCGGACGGGAAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGGCGTGTATCAGTACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTAc                                                                                                        >  5:289016/1‑149 (MQ=255)
                                             gggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACt                                                                                                       <  3:117812/147‑1 (MQ=255)
                                             gggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGa                                                                                                     >  6:181136/1‑149 (MQ=255)
                                              ggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                    >  7:153742/1‑149 (MQ=255)
                                                  tGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGt                                                                                                <  5:181136/149‑1 (MQ=255)
                                                     aTGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                    <  7:243493/142‑1 (MQ=255)
                                                     aTGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                    >  8:243493/1‑142 (MQ=255)
                                                      tGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtg                                                                                            >  3:224943/1‑149 (MQ=255)
                                                        tCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                          <  8:276480/149‑1 (MQ=255)
                                                         ccatcTGCCCGTCCGGCAGATGGGCAACTCCGAAGTCGGTCATATGAGCCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGGGACGGCGAGTTCTTCGAAAACCCGGTACTGAGCGGTGCTGTgg                                                                                         >  4:210964/5‑149 (MQ=255)
                                                          ggCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTgg                                                                                         <  2:171285/148‑1 (MQ=255)
                                                            ccTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGAc                                                                                       >  1:155803/1‑148 (MQ=255)
                                                                   gACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAATACCCGGTACTGACCGGTGCTGTGGACAAGGCAGt                                                                               <  1:197138/149‑1 (MQ=255)
                                                                    aCGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                          >  5:18127/1‑137 (MQ=255)
                                                                    aCGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                          <  6:18127/137‑1 (MQ=255)
                                                                     cGGGCATATGGGCAACTCCGAAGTCGGTCATATGAAACTAGGCGCAGGTCGCGTCGTGTATCAGGACTTCACTCGGGTGACCAAGGCCATCCGCGACGGCTAGTTCTTCGAAAACCCGGTACTGACCTGTGCTGTGTAAAAGGCAGTCg                                                                             <  6:289016/149‑1 (MQ=255)
                                                                         cAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCTGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGc                                                                         <  5:114152/149‑1 (MQ=255)
                                                                          aGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCt                                                                        >  4:260544/1‑149 (MQ=255)
                                                                           gATGGGCACCGCCGAAGTCGGTCATATGCACCTCGGCGCAGGTCGCGTCGTGTATCAGGCCTTCACCCGGGTGACCAAGCCCATCCGCGACGGCGACTTCTTCGAAAACCCGGTACTGACCGGTGCTGCGGACAAGGCAGTCGGTGCTg                                                                       <  8:133957/149‑1 (MQ=255)
                                                                           cctGGGCCACTCCTCAGTCGGCCATAGTAACCTCGGCGCAGGTCGCGTCGTCTATCTGGACTTCACGCGTGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTg                                                                       <  4:224943/147‑1 (MQ=255)
                                                                                 cAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAgg                                                                 >  7:275833/1‑149 (MQ=255)
                                                                                       cGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                            >  1:144970/1‑148 (MQ=255)
                                                                                       cGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGc                                                           <  8:275833/149‑1 (MQ=255)
                                                                                                  atatGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGa                                                >  6:66643/1‑149 (MQ=255)
                                                                                                  atatGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGa                                                <  2:155803/149‑1 (MQ=255)
                                                                                                    atcaaCCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAACGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGAGCACATCCTCGGAct                                              >  5:126999/4‑149 (MQ=255)
                                                                                                     tGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGATAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGActg                                             >  4:15254/1‑149 (MQ=255)
                                                                                                      gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                            <  5:6228/133‑1 (MQ=255)
                                                                                                      gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                            >  6:6228/1‑133 (MQ=255)
                                                                                                         ccTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGActgctg                                          >  4:94507/1‑148 (MQ=255)
                                                                                                          cTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTc                                        >  1:229675/1‑149 (MQ=255)
                                                                                                          cTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACAGCAGTc                                        >  5:755/1‑149 (MQ=255)
                                                                                                             ggCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACAGCAGTCCGa                                     <  6:755/149‑1 (MQ=255)
                                                                                                                  aGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                <  5:66643/149‑1 (MQ=255)
                                                                                                                  aGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                >  5:182860/1‑149 (MQ=255)
                                                                                                                    gTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGATAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATggtggt                              <  3:15254/149‑1 (MQ=255)
                                                                                                                             tgtATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTTCTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGc                     >  5:239530/1‑149 (MQ=255)
                                                                                                                                 tCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGccac                  >  8:195312/1‑148 (MQ=255)
                                                                                                                                       cTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGACAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGAc            >  1:94533/1‑148 (MQ=255)
                                                                                                                                        ttCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCa          >  3:294738/1‑149 (MQ=255)
                                                                                                                                          gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTAcaca                       <  6:104243/132‑1 (MQ=255)
                                                                                                                                          gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTAcaca                       >  5:104243/3‑134 (MQ=255)
                                                                                                                                          gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACCCAGCCCCCAGGACCCCt        >  7:285939/3‑149 (MQ=255)
                                                                                                                                           tcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                >  3:158697/2‑124 (MQ=255)
                                                                                                                                           tcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                <  4:158697/123‑1 (MQ=255)
                                                                                                                                           aCGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTacac                        >  8:212451/1‑132 (MQ=255)
                                                                                                                                           aCGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTacac                        <  7:212451/132‑1 (MQ=255)
                                                                                                                                             gcgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTac                          <  3:258794/128‑1 (MQ=255)
                                                                                                                                             gcgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTac                          >  4:258794/1‑128 (MQ=255)
                                                                                                                                              cgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggt    <  3:260544/149‑1 (MQ=255)
                                                                                                                                                ggTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg  <  8:153742/149‑1 (MQ=255)
                                                                                                                                                ggTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg  <  2:257112/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGACCGCCTGTGCGCCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGG  >  NC_002947/5761053‑5761345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑