Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,761,211 T→C 7.6% I88T (ATC→ACC)  gpmI → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,761,2110TC 7.6% 184.9 / 5.0 66I88T (ATC→ACC) gpmI2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base T (33/28);  new base C (3/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATGG  >  NC_002947/5761067‑5761351
                                                                                                                                                |                                                                                                                                            
gccaccAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCcgc                                                                                                                                          >  2:197138/5‑149 (MQ=255)
ccACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCcgc                                                                                                                                          >  7:133957/1‑149 (MQ=255)
  aCCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGa                                                                                                                                        >  7:276480/1‑149 (MQ=255)
        ccGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGc                                                                                                                                    >  4:117812/1‑147 (MQ=255)
               gCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGttctt                                                                                                                            <  7:134353/148‑1 (MQ=255)
                         cGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCgg                                                                                                                 <  7:3872/149‑1 (MQ=255)
                         cGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCgg                                                                                                                 <  6:98095/149‑1 (MQ=255)
                            cTCGGGCATGGATGTCGGCCTGCCGGACGGGAAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGGCGTGTATCAGTACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTAc                                                                                                              >  5:289016/1‑149 (MQ=255)
                               gggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACt                                                                                                             <  3:117812/147‑1 (MQ=255)
                               gggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGa                                                                                                           >  6:181136/1‑149 (MQ=255)
                                ggCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                          >  7:153742/1‑149 (MQ=255)
                                    tGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGt                                                                                                      <  5:181136/149‑1 (MQ=255)
                                       aTGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                          <  7:243493/142‑1 (MQ=255)
                                       aTGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAc                                                                                                          >  8:243493/1‑142 (MQ=255)
                                        tGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtg                                                                                                  >  3:224943/1‑149 (MQ=255)
                                          tCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                                <  8:276480/149‑1 (MQ=255)
                                           ccatcTGCCCGTCCGGCAGATGGGCAACTCCGAAGTCGGTCATATGAGCCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGGGACGGCGAGTTCTTCGAAAACCCGGTACTGAGCGGTGCTGTgg                                                                                               >  4:210964/5‑149 (MQ=255)
                                            ggCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTgg                                                                                               <  2:171285/148‑1 (MQ=255)
                                              ccTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGAc                                                                                             >  1:155803/1‑148 (MQ=255)
                                                     gACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAATACCCGGTACTGACCGGTGCTGTGGACAAGGCAGt                                                                                     <  1:197138/149‑1 (MQ=255)
                                                      aCGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                                <  6:18127/137‑1 (MQ=255)
                                                      aCGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCtgtg                                                                                                >  5:18127/1‑137 (MQ=255)
                                                       cGGGCATATGGGCAACTCCGAAGTCGGTCATATGAAACTAGGCGCAGGTCGCGTCGTGTATCAGGACTTCACTCGGGTGACCAAGGCCATCCGCGACGGCTAGTTCTTCGAAAACCCGGTACTGACCTGTGCTGTGTAAAAGGCAGTCg                                                                                   <  6:289016/149‑1 (MQ=255)
                                                           cAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCTGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGc                                                                               <  5:114152/149‑1 (MQ=255)
                                                            aGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCt                                                                              >  4:260544/1‑149 (MQ=255)
                                                             gATGGGCACCGCCGAAGTCGGTCATATGCACCTCGGCGCAGGTCGCGTCGTGTATCAGGCCTTCACCCGGGTGACCAAGCCCATCCGCGACGGCGACTTCTTCGAAAACCCGGTACTGACCGGTGCTGCGGACAAGGCAGTCGGTGCTg                                                                             <  8:133957/149‑1 (MQ=255)
                                                             cctGGGCCACTCCTCAGTCGGCCATAGTAACCTCGGCGCAGGTCGCGTCGTCTATCTGGACTTCACGCGTGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTg                                                                             <  4:224943/147‑1 (MQ=255)
                                                                   cAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAgg                                                                       >  7:275833/1‑149 (MQ=255)
                                                                         cGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                                  >  1:144970/1‑148 (MQ=255)
                                                                         cGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGc                                                                 <  8:275833/149‑1 (MQ=255)
                                                                                    atatGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGa                                                      <  2:155803/149‑1 (MQ=255)
                                                                                    atatGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGa                                                      >  6:66643/1‑149 (MQ=255)
                                                                                      atcaaCCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGAACGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGAGCACATCCTCGGAct                                                    >  5:126999/4‑149 (MQ=255)
                                                                                       tGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGATAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGActg                                                   >  4:15254/1‑149 (MQ=255)
                                                                                        gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                                  <  5:6228/133‑1 (MQ=255)
                                                                                        gAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTg                                                                  >  6:6228/1‑133 (MQ=255)
                                                                                           ccTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGActgctg                                                >  4:94507/1‑148 (MQ=255)
                                                                                            cTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTc                                              >  1:229675/1‑149 (MQ=255)
                                                                                            cTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACAGCAGTc                                              >  5:755/1‑149 (MQ=255)
                                                                                               ggCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACAGCAGTCCGa                                           <  6:755/149‑1 (MQ=255)
                                                                                                    aGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      >  5:182860/1‑149 (MQ=255)
                                                                                                    aGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      <  5:66643/149‑1 (MQ=255)
                                                                                                      gTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGATAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATggtggt                                    <  3:15254/149‑1 (MQ=255)
                                                                                                               tgtATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTTCTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGc                           >  5:239530/1‑149 (MQ=255)
                                                                                                                   tCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGccac                        >  8:195312/1‑148 (MQ=255)
                                                                                                                         cTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGACAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGAc                  >  1:94533/1‑148 (MQ=255)
                                                                                                                          ttCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCa                >  3:294738/1‑149 (MQ=255)
                                                                                                                            gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTAcaca                             >  5:104243/3‑134 (MQ=255)
                                                                                                                            gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTAcaca                             <  6:104243/132‑1 (MQ=255)
                                                                                                                            gtcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACCCAGCCCCCAGGACCCCt              >  7:285939/3‑149 (MQ=255)
                                                                                                                             tcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      <  4:158697/123‑1 (MQ=255)
                                                                                                                             tcgcgGATGGCCTTGGTCACCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      >  3:158697/2‑124 (MQ=255)
                                                                                                                             aCGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTacac                              <  7:212451/132‑1 (MQ=255)
                                                                                                                             aCGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTacac                              >  8:212451/1‑132 (MQ=255)
                                                                                                                               gcgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTac                                <  3:258794/128‑1 (MQ=255)
                                                                                                                               gcgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTac                                >  4:258794/1‑128 (MQ=255)
                                                                                                                                cgGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggt          <  3:260544/149‑1 (MQ=255)
                                                                                                                                  ggTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg        <  2:257112/149‑1 (MQ=255)
                                                                                                                                  ggTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTtggtgg        <  8:153742/149‑1 (MQ=255)
                                                                                                                                      aCCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACATAGCCACCAGGACCACTTGGTGGCCAt    <  3:210964/149‑1 (MQ=255)
                                                                                                                                       ccAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATg   >  6:251678/1‑149 (MQ=255)
                                                                                                                                        cAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGTAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATgg  >  5:23241/1‑149 (MQ=255)
                                                                                                                                         aaGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTgg                                                                            >  4:98585/1‑74 (MQ=255)
                                                                                                                                         aaGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTgg                                                                            <  3:98585/74‑1 (MQ=255)
                                                                                                                                             ccATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      >  7:15625/1‑108 (MQ=255)
                                                                                                                                             ccATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGAtggtg                                      <  8:15625/108‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CCACCCAGCCGCATGGCCTCATTTCCGGCTCGGGCATGGATGTCGGCCTGCCGGACGGGCAGATGGGCAACTCCGAAGTCGGTCATATGAACCTCGGCGCAGGTCGCGTCGTGTATCAGGACTTCACGCGGGTGACCAAGGCCATCCGCGACGGCGAGTTCTTCGAAAACCCGGTACTGACCGGTGCTGTGGACAAGGCAGTCGGTGCTGGCAAGGCCGTGCACATCCTCGGACTGCTGTCCGATGGTGGTGTACACAGCCACCAGGACCACTTGGTGGCCATGG  >  NC_002947/5761067‑5761351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑