Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,791,620	A→C	7.4%	L33R (CTG→CGG)	hemE ←	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,791,620	0	A	C	7.4%	208.5 / 6.3	68	L33R (CTG→CGG)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base C (3/2);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

GGAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGC  >  NC_002947/5791473‑5791762                                                                                                                                                    |                                                                                                                                                ggAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAg                                                                                                                                                <  3:295785/149‑1 (MQ=255)  gAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAgg                                                                                                                                               <  7:95871/149‑1 (MQ=255)        tAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcgg                                                                                                                                         <  3:212615/149‑1 (MQ=255)           ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccg                                                                                                                                      <  5:288575/149‑1 (MQ=255)           ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                       >  8:77712/1‑148 (MQ=255)           ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                       <  7:77712/148‑1 (MQ=255)            gATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccgg                                                                                                                                     <  5:249288/149‑1 (MQ=255)               gTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCt                                                                                                                                  >  8:100259/1‑149 (MQ=255)                         aGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCA‑GCTGGCCGCCCCGGGCAACTGCGGGGTCAAGCACTGGCGCATGCAGTCACCCGCCGTGGCGCGGCTTGCGCGGGACGGCGGGCCGTAGCCGCCGGACGGGCGCcacatc                                                                                                                       >  8:202807/1‑149 (MQ=255)                              cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                               >  4:237492/1‑131 (MQ=255)                              cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                               <  3:237495/137‑7 (MQ=255)                              cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                               <  3:237492/137‑7 (MQ=255)                              cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtaac                                                                                                                               >  4:237495/1‑131 (MQ=255)                                       cAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGa                                                                                                          >  1:95915/1‑149 (MQ=255)                                       cAGAGGGTAGCGGTCCAGCGGCTGCTGGGTGACGTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGTCCTTGGCGCGGCTGGCGCGGTACTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACGGGGGtt                                                                                                           <  7:288614/148‑2 (MQ=255)                                        aGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGAc                                                                                                         >  5:132423/1‑149 (MQ=255)                                            ggTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCTGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCt                                                                                                     <  4:45817/149‑1 (MQ=255)                                              tAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTAc                                                                                                   >  8:44089/1‑149 (MQ=255)                                                gCGGTCCAGCGGCTGGAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACgg                                                                                                 >  5:93773/1‑149 (MQ=255)                                                 cGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACggg                                                                                                <  7:100259/149‑1 (MQ=255)                                                      cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTTCTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                           >  3:247766/1‑149 (MQ=255)                                                      cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                           >  1:170844/1‑149 (MQ=255)                                                             tGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAg                                                                                    >  1:273015/1‑149 (MQ=255)                                                               cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                   >  8:28987/1‑148 (MQ=255)                                                               cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                   <  7:28987/148‑1 (MQ=255)                                                               cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                   <  2:95915/148‑1 (MQ=255)                                                                aGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGc                                                                                 <  7:14197/149‑1 (MQ=255)                                                                      aCCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCa                                                                            <  6:93773/148‑1 (MQ=255)                                                                        cTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                          >  3:98602/1‑148 (MQ=255)                                                                         tCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGa                                                                         >  4:105865/1‑148 (MQ=255)                                                                              ggCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGAt                                                                   <  8:216287/149‑1 (MQ=255)                                                                                 gAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGt                                                                >  5:169910/1‑149 (MQ=255)                                                                                  aaCTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGtt                                                               <  7:53717/149‑1 (MQ=255)                                                                                         ggTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                        >  2:196572/1‑149 (MQ=255)                                                                                                 cacaGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCCTCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCAt                                                >  8:238900/1‑149 (MQ=255)                                                                                                      gCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCg‑g                                          <  6:222752/149‑1 (MQ=255)                                                                                                        tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTGCGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                        >  1:224817/1‑149 (MQ=255)                                                                                                        tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                        >  5:217796/1‑149 (MQ=255)                                                                                                          aTGAAGTCACCGGCGTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCGACCCCGGGGGGACGTCTACGCGTAGCTTGAGCAGTGGAGGCAGGAAACGATCGTTCTTCAGGGCAGACATGTCG‑GCTTc                                      >  8:197754/1‑149 (MQ=255)                                                                                                          aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                          >  1:52051/1‑114 (MQ=255)                                                                                                          aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                          <  2:52051/114‑1 (MQ=255)                                                                                                              aGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCgg                                   >  2:31546/1‑148 (MQ=255)                                                                                                                  aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                              >  3:92370/1‑149 (MQ=255)                                                                                                                  aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                              >  2:244694/1‑149 (MQ=255)                                                                                                                    cGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATa                            <  2:170844/149‑1 (MQ=255)                                                                                                                      gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                          >  5:79494/1‑149 (MQ=255)                                                                                                                      gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                          >  5:75032/1‑149 (MQ=255)                                                                                                                         ttGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTATTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                       >  4:143655/1‑149 (MQ=255)                                                                                                                           ggCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                       <  2:273015/147‑1 (MQ=255)                                                                                                                            gcgcGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCggg                     >  6:230245/1‑148 (MQ=255)                                                                                                                             cgcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCa                   >  4:197080/1‑149 (MQ=255)                                                                                                                              gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                        <  6:46993/112‑1 (MQ=255)                                                                                                                              gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                        >  5:46993/1‑112 (MQ=255)                                                                                                                                  cTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtc              <  3:197080/149‑1 (MQ=255)                                                                                                                                     gcgcGGTACTCCGTCATGTAGCGGCCTGCGTGGCTCATCATCCACACCGGTGTGACGTCTACGTGTTGCTTGATCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc            <  6:169910/148‑1 (MQ=255)                                                                                                                                     gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGTCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc            <  3:105865/148‑1 (MQ=255)                                                                                                                                     gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa           <  4:98602/149‑1 (MQ=255)                                                                                                                                     gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa           <  4:92370/149‑1 (MQ=255)                                                                                                                                      cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                           <  2:277676/85‑1 (MQ=255)                                                                                                                                      cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                           >  1:277676/1‑85 (MQ=255)                                                                                                                                      cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtct             >  3:274964/1‑146 (MQ=255)                                                                                                                                      cgcgGTACTCCGGCAGCTAGCGGCCGGCCTCGCGCATCATCCACACCGGGCTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACCATCGTTCTTCAGCGCAGTCATGTCG‑GCATCCGGAAAAATACTGCCGGCATTAtct             >  3:279353/1‑146 (MQ=255)                                                                                                                                        cgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCGTGCATCCGGAAAAATAGTGCGGGCATTTTCTCAGa         <  1:196572/149‑1 (MQ=255)                                                                                                                                             cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCa    <  7:44089/148‑1 (MQ=255)                                                                                                                                             cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAATCGATCGTTCTTCCGTGCAGTCATGTCG‑GCATCCTGAAAAATAGTGCGGGCATTTTCTCAGTCACCa    <  4:247766/148‑1 (MQ=255)                                                                                                                                              tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                 <  7:252404/87‑1 (MQ=255)                                                                                                                                              tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                 >  8:252404/1‑87 (MQ=255)                                                                                                                                              tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAGTAGTGCGGGCATTTTCTCAGACACCAgc  >  6:78099/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                GGAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGC  >  NC_002947/5791473‑5791762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑