Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,791,622	G→C	7.6%	Y32* (TAC→TAG)	hemE ←	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,791,622	0	G	C	7.6%	200.5 / 5.6	66	Y32* (TAC→TAG)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base C (3/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAGG  >  NC_002947/5791474‑5791770                                                                                                                                                     |                                                                                                                                                      gAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAgg                                                                                                                                                       <  7:95871/149‑1 (MQ=255)       tAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcgg                                                                                                                                                 <  3:212615/149‑1 (MQ=255)          ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccg                                                                                                                                              <  5:288575/149‑1 (MQ=255)          ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                               >  8:77712/1‑148 (MQ=255)          ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                               <  7:77712/148‑1 (MQ=255)           gATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccgg                                                                                                                                             <  5:249288/149‑1 (MQ=255)              gTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCt                                                                                                                                          >  8:100259/1‑149 (MQ=255)                        aGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCA‑GCTGGCCGCCCCGGGCAACTGCGGGGTCAAGCACTGGCGCATGCAGTCACCCGCCGTGGCGCGGCTTGCGCGGGACGGCGGGCCGTAGCCGCCGGACGGGCGCcacatc                                                                                                                               >  8:202807/1‑149 (MQ=255)                             cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                       >  4:237492/1‑131 (MQ=255)                             cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                       <  3:237495/137‑7 (MQ=255)                             cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                       <  3:237492/137‑7 (MQ=255)                             cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtaac                                                                                                                                       >  4:237495/1‑131 (MQ=255)                                      cAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGa                                                                                                                  >  1:95915/1‑149 (MQ=255)                                      cAGAGGGTAGCGGTCCAGCGGCTGCTGGGTGACGTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGTCCTTGGCGCGGCTGGCGCGGTACTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACGGGGGtt                                                                                                                   <  7:288614/148‑2 (MQ=255)                                       aGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGAc                                                                                                                 >  5:132423/1‑149 (MQ=255)                                           ggTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCTGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCt                                                                                                             <  4:45817/149‑1 (MQ=255)                                             tAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTAc                                                                                                           >  8:44089/1‑149 (MQ=255)                                               gCGGTCCAGCGGCTGGAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACgg                                                                                                         >  5:93773/1‑149 (MQ=255)                                                cGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACggg                                                                                                        <  7:100259/149‑1 (MQ=255)                                                     cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTTCTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                                   >  3:247766/1‑149 (MQ=255)                                                     cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                                   >  1:170844/1‑149 (MQ=255)                                                            tGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAg                                                                                            >  1:273015/1‑149 (MQ=255)                                                              cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                           <  7:28987/148‑1 (MQ=255)                                                              cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                           <  2:95915/148‑1 (MQ=255)                                                              cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                           >  8:28987/1‑148 (MQ=255)                                                               aGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGc                                                                                         <  7:14197/149‑1 (MQ=255)                                                                     aCCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCa                                                                                    <  6:93773/148‑1 (MQ=255)                                                                       cTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                  >  3:98602/1‑148 (MQ=255)                                                                        tCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGa                                                                                 >  4:105865/1‑148 (MQ=255)                                                                             ggCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGAt                                                                           <  8:216287/149‑1 (MQ=255)                                                                                gAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGt                                                                        >  5:169910/1‑149 (MQ=255)                                                                                 aaCTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGtt                                                                       <  7:53717/149‑1 (MQ=255)                                                                                        ggTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                >  2:196572/1‑149 (MQ=255)                                                                                                cacaGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCCTCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCAt                                                        >  8:238900/1‑149 (MQ=255)                                                                                                     gCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCg‑g                                                  <  6:222752/149‑1 (MQ=255)                                                                                                       tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTGCGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                                >  1:224817/1‑149 (MQ=255)                                                                                                       tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                                >  5:217796/1‑149 (MQ=255)                                                                                                         aTGAAGTCACCGGCGTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCGACCCCGGGGGGACGTCTACGCGTAGCTTGAGCAGTGGAGGCAGGAAACGATCGTTCTTCAGGGCAGACATGTCG‑GCTTc                                              >  8:197754/1‑149 (MQ=255)                                                                                                         aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                  >  1:52051/1‑114 (MQ=255)                                                                                                         aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                  <  2:52051/114‑1 (MQ=255)                                                                                                             aGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCgg                                           >  2:31546/1‑148 (MQ=255)                                                                                                                 aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                                      >  2:244694/1‑149 (MQ=255)                                                                                                                 aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                                      >  3:92370/1‑149 (MQ=255)                                                                                                                   cGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATa                                    <  2:170844/149‑1 (MQ=255)                                                                                                                     gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                                  >  5:79494/1‑149 (MQ=255)                                                                                                                     gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                                  >  5:75032/1‑149 (MQ=255)                                                                                                                        ttGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTATTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                               >  4:143655/1‑149 (MQ=255)                                                                                                                          ggCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                               <  2:273015/147‑1 (MQ=255)                                                                                                                           gcgcGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCggg                             >  6:230245/1‑148 (MQ=255)                                                                                                                            cgcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCa                           >  4:197080/1‑149 (MQ=255)                                                                                                                             gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                >  5:46993/1‑112 (MQ=255)                                                                                                                             gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                <  6:46993/112‑1 (MQ=255)                                                                                                                                 cTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtc                      <  3:197080/149‑1 (MQ=255)                                                                                                                                    gcgcGGTACTCCGTCATGTAGCGGCCTGCGTGGCTCATCATCCACACCGGTGTGACGTCTACGTGTTGCTTGATCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc                    <  6:169910/148‑1 (MQ=255)                                                                                                                                    gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGTCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc                    <  3:105865/148‑1 (MQ=255)                                                                                                                                    gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa                   <  4:98602/149‑1 (MQ=255)                                                                                                                                    gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa                   <  4:92370/149‑1 (MQ=255)                                                                                                                                     cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                                   >  1:277676/1‑85 (MQ=255)                                                                                                                                     cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                                   <  2:277676/85‑1 (MQ=255)                                                                                                                                     cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtct                     >  3:274964/1‑146 (MQ=255)                                                                                                                                     cgcgGTACTCCGGCAGCTAGCGGCCGGCCTCGCGCATCATCCACACCGGGCTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACCATCGTTCTTCAGCGCAGTCATGTCG‑GCATCCGGAAAAATACTGCCGGCATTAtct                     >  3:279353/1‑146 (MQ=255)                                                                                                                                       cgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCGTGCATCCGGAAAAATAGTGCGGGCATTTTCTCAGa                 <  1:196572/149‑1 (MQ=255)                                                                                                                                            cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCa            <  7:44089/148‑1 (MQ=255)                                                                                                                                            cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAATCGATCGTTCTTCCGTGCAGTCATGTCG‑GCATCCTGAAAAATAGTGCGGGCATTTTCTCAGTCACCa            <  4:247766/148‑1 (MQ=255)                                                                                                                                             tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                         <  7:252404/87‑1 (MQ=255)                                                                                                                                             tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                         >  8:252404/1‑87 (MQ=255)                                                                                                                                             tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAGTAGTGCGGGCATTTTCTCAGACACCAgc          >  6:78099/1‑149 (MQ=255)                                                                                                                                                     gTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAgg  >  8:103890/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GAATGGTAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAGG  >  NC_002947/5791474‑5791770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑