Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,791,625	G→C	5.9%	R31R (CGC→CGG)	hemE ←	uroporphyrinogen decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,791,625	0	G	C	5.9%	215.0 / 2.3	67	R31R (CGC→CGG)	hemE	uroporphyrinogen decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (35/28);  new base C (2/2);  total (37/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

TAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAGGCAC  >  NC_002947/5791480‑5791773                                                                                                                                                  |                                                                                                                                                      tAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcgg                                                                                                                                                    <  3:212615/149‑1 (MQ=255)    ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccg                                                                                                                                                 <  5:288575/149‑1 (MQ=255)    ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                                  >  8:77712/1‑148 (MQ=255)    ggATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggcc                                                                                                                                                  <  7:77712/148‑1 (MQ=255)     gATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGcggccgg                                                                                                                                                <  5:249288/149‑1 (MQ=255)        gTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCt                                                                                                                                             >  8:100259/1‑149 (MQ=255)                  aGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCA‑GCTGGCCGCCCCGGGCAACTGCGGGGTCAAGCACTGGCGCATGCAGTCACCCGCCGTGGCGCGGCTTGCGCGGGACGGCGGGCCGTAGCCGCCGGACGGGCGCcacatc                                                                                                                                  >  8:202807/1‑149 (MQ=255)                       cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                          >  4:237492/1‑131 (MQ=255)                       cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                          <  3:237495/137‑7 (MQ=255)                       cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtacc                                                                                                                                          <  3:237492/137‑7 (MQ=255)                       cGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCCGCTACctgccggagtaac                                                                                                                                          >  4:237495/1‑131 (MQ=255)                                cAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGa                                                                                                                     >  1:95915/1‑149 (MQ=255)                                cAGAGGGTAGCGGTCCAGCGGCTGCTGGGTGACGTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGTCCTTGGCGCGGCTGGCGCGGTACTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACGGGGGtt                                                                                                                      <  7:288614/148‑2 (MQ=255)                                 aGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGAc                                                                                                                    >  5:132423/1‑149 (MQ=255)                                     ggTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCTGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCt                                                                                                                <  4:45817/149‑1 (MQ=255)                                       tAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTAc                                                                                                              >  8:44089/1‑149 (MQ=255)                                         gCGGTCCAGCGGCTGGAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACgg                                                                                                            >  5:93773/1‑149 (MQ=255)                                          cGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACggg                                                                                                           <  7:100259/149‑1 (MQ=255)                                               cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTTCTCAGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                                      >  3:247766/1‑149 (MQ=255)                                               cAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCt                                                                                                      >  1:170844/1‑149 (MQ=255)                                                      tGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAg                                                                                               >  1:273015/1‑149 (MQ=255)                                                        cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                              <  7:28987/148‑1 (MQ=255)                                                        cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                              <  2:95915/148‑1 (MQ=255)                                                        cAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGt                                                                                              >  8:28987/1‑148 (MQ=255)                                                         aGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGc                                                                                            <  7:14197/149‑1 (MQ=255)                                                               aCCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCa                                                                                       <  6:93773/148‑1 (MQ=255)                                                                 cTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                     >  3:98602/1‑148 (MQ=255)                                                                  tCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGa                                                                                    >  4:105865/1‑148 (MQ=255)                                                                       ggCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGAt                                                                              <  8:216287/149‑1 (MQ=255)                                                                          gAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGt                                                                           >  5:169910/1‑149 (MQ=255)                                                                           aaCTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGtt                                                                          <  7:53717/149‑1 (MQ=255)                                                                                  ggTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                   >  2:196572/1‑149 (MQ=255)                                                                                          cacaGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCCTCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCAt                                                           >  8:238900/1‑149 (MQ=255)                                                                                               gCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCg‑g                                                     <  6:222752/149‑1 (MQ=255)                                                                                                 tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTGCGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                                   >  1:224817/1‑149 (MQ=255)                                                                                                 tCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCa                                                   >  5:217796/1‑149 (MQ=255)                                                                                                   aTGAAGTCACCGGCGTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCGACCCCGGGGGGACGTCTACGCGTAGCTTGAGCAGTGGAGGCAGGAAACGATCGTTCTTCAGGGCAGACATGTCG‑GCTTc                                                 >  8:197754/1‑149 (MQ=255)                                                                                                   aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                     <  2:52051/114‑1 (MQ=255)                                                                                                   aTGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAgg                                                                                     >  1:52051/1‑114 (MQ=255)                                                                                                       aGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCgg                                              >  2:31546/1‑148 (MQ=255)                                                                                                           aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                                         >  2:244694/1‑149 (MQ=255)                                                                                                           aCCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGaaaaa                                         >  3:92370/1‑149 (MQ=255)                                                                                                             cGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATa                                       <  2:170844/149‑1 (MQ=255)                                                                                                               gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                                     >  5:79494/1‑149 (MQ=255)                                                                                                               gCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGt                                     >  5:75032/1‑149 (MQ=255)                                                                                                                  ttGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTATTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                                  >  4:143655/1‑149 (MQ=255)                                                                                                                    ggCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCg                                  <  2:273015/147‑1 (MQ=255)                                                                                                                     gcgcGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCggg                                >  6:230245/1‑148 (MQ=255)                                                                                                                      cgcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCa                              >  4:197080/1‑149 (MQ=255)                                                                                                                       gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                   <  6:46993/112‑1 (MQ=255)                                                                                                                       gcgGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAgg                                                                   >  5:46993/1‑112 (MQ=255)                                                                                                                           cTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtc                         <  3:197080/149‑1 (MQ=255)                                                                                                                              gcgcGGTACTCCGTCATGTAGCGGCCTGCGTGGCTCATCATCCACACCGGTGTGACGTCTACGTGTTGCTTGATCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc                       <  6:169910/148‑1 (MQ=255)                                                                                                                              gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGTCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtctc                       <  3:105865/148‑1 (MQ=255)                                                                                                                              gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa                      <  4:98602/149‑1 (MQ=255)                                                                                                                              gcgcGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCa                      <  4:92370/149‑1 (MQ=255)                                                                                                                               cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                                      >  1:277676/1‑85 (MQ=255)                                                                                                                               cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAg                                                                                      <  2:277676/85‑1 (MQ=255)                                                                                                                               cgcgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTtct                        >  3:274964/1‑146 (MQ=255)                                                                                                                               cgcgGTACTCCGGCAGCTAGCGGCCGGCCTCGCGCATCATCCACACCGGGCTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACCATCGTTCTTCAGCGCAGTCATGTCG‑GCATCCGGAAAAATACTGCCGGCATTAtct                        >  3:279353/1‑146 (MQ=255)                                                                                                                                 cgGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCGTGCATCCGGAAAAATAGTGCGGGCATTTTCTCAGa                    <  1:196572/149‑1 (MQ=255)                                                                                                                                      cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGGCGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCa               <  7:44089/148‑1 (MQ=255)                                                                                                                                      cTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAATCGATCGTTCTTCCGTGCAGTCATGTCG‑GCATCCTGAAAAATAGTGCGGGCATTTTCTCAGTCACCa               <  4:247766/148‑1 (MQ=255)                                                                                                                                       tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                            <  7:252404/87‑1 (MQ=255)                                                                                                                                       tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCg                                                                            >  8:252404/1‑87 (MQ=255)                                                                                                                                       tCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAGTAGTGCGGGCATTTTCTCAGACACCAgc             >  6:78099/1‑149 (MQ=255)                                                                                                                                               gTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAgg     >  8:103890/1‑149 (MQ=255)                                                                                                                                                  gCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAGGCAc  >  8:290861/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                      TAAGGATGTCCGAGAACAGGATCGCTGCATCCAGCGGGTAGCGGTCCAGCGGCTGCAGGGTGACCTCGCAGGCGAACTGCGGGTTCATGCACAGGCTCATGAAGTCACCGGCCTTGGCGCGGCTGGCGCGGTACTCCGGCAGGTAGCGGCCGGCCTGGCGCATCATCCACACCGGGGTGACGTCTACGGGTTGCTTGAGCAGTGCACGCAGGAAACGATCGTTCTTCAGGGCAGTCATGTCG‑GCATCCGGAAAAATAGTGCGGGCATTTTCTCAGACACCAGCGCAAAAGGCAC  >  NC_002947/5791480‑5791773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑