Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 441,611 C→T 12.0% V164V (GTC→GTT) ‡ bioF → 8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947441,6110CT12.0% 127.7 / 7.3 50V164V (GTC→GTT) ‡bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base C (27/17);  new base T (3/3);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

GGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGCCAAAG  >  NC_002947/441475‑441756
                                                                                                                                          |                                                                                                                                                 
ggCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCtg                                                                                                                                       <  1:17374/149‑1 (MQ=255)
     ggCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTggtggt                                                                                                                                  >  6:18658/1‑149 (MQ=255)
                    cAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCCATGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCgtgt                                                                                                                    <  1:62957/149‑1 (MQ=255)
                    cAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCgtgt                                                                                                                    <  3:253697/148‑1 (MQ=255)
                                   cATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGTGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGa                                                                                                    >  4:235224/1‑149 (MQ=255)
                                        cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGAc                                                                                                   <  6:210934/145‑1 (MQ=255)
                                        cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGAc                                                                                                   >  5:210934/1‑145 (MQ=255)
                                        cTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTgg                                                                                               >  5:210956/1‑149 (MQ=255)
                                         tCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCgg                                                                                                                                                 >  3:154590/1‑98 (MQ=255)
                                         tCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCgg                                                                                                                                                 <  4:154590/98‑1 (MQ=255)
                                           gTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCTACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGcc                                                                                             <  7:140148/148‑1 (MQ=255)
                                             tGCTGGATGGCGGGTTGCTCAGAGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCAAGCCT‑GGCCAGCCGCC‑TTGACAAGGCGGTCGGCAATACACTGCTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGAc                                                                                          >  5:215821/1‑149 (MQ=255)
                                              gCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGAc                                                                                          <  1:261074/148‑1 (MQ=255)
                                                 ggATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGc                                                                                      >  2:111523/1‑149 (MQ=255)
                                                       cGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGgcgc                                                                                <  5:18658/149‑1 (MQ=255)
                                                          gtcgCTCAGTGGCGCCCGCTTCTACCGCTACCTGCACAACCACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGCACGGTGACCTGGCCGACCTGCCGGCGCTgg                                                                             <  6:253230/146‑1 (MQ=255)
                                                                 aGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCTGGGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATg                                                                       >  1:71991/1‑149 (MQ=255)
                                                                     gtgCCCGGGTCAACCGCTACCTGCACTACGAGCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACTGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGc                                                                   <  6:215821/148‑1 (MQ=255)
                                                                     gtgCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGcc                                                                  >  2:30038/1‑149 (MQ=255)
                                                                        cccGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGt                                                               >  8:156180/1‑149 (MQ=255)
                                                                         ccGCTTCAACTGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTg                                                              >  2:307858/1‑149 (MQ=255)
                                                                            cTTCAACCGCTACCTGCACACCGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGccc                                                           >  5:54713/1‑149 (MQ=255)
                                                                                 aCCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGt                                                      >  8:14859/1‑149 (MQ=255)
                                                                                         cTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCt                                              >  6:254368/1‑149 (MQ=255)
                                                                                            cacaACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGa                                            <  6:210956/148‑1 (MQ=255)
                                                                                                ggcggctggCCAGGCT‑GGCCGGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTc                                       >  2:259265/8‑149 (MQ=255)
                                                                                                              cT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg                         >  3:208603/1‑149 (MQ=255)
                                                                                                              at‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg                         <  1:258928/148‑1 (MQ=255)
                                                                                                               t‑GGCCAGCAGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATTGACGGTGACCTGGCCGACATGCCGGCGCTGGCCAATGTCGCCCGACCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATgg                         >  2:265083/1‑148 (MQ=255)
                                                                                                                ttgccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGc                        >  6:57015/8‑149 (MQ=255)
                                                                                                                   ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGcc                                                   <  1:144948/115‑1 (MQ=255)
                                                                                                                   ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGcc                                                   >  2:144948/5‑119 (MQ=255)
                                                                                                                   ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGAt                                           <  7:83426/123‑1 (MQ=255)
                                                                                                                   ccgaccgcc‑TTGTCCAGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGAt                                           >  8:83426/5‑127 (MQ=255)
                                                                                                                    cAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg                    <  7:156180/149‑1 (MQ=255)
                                                                                                                    cAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg                    >  8:286158/1‑149 (MQ=255)
                                                                                                                         gcc‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAcc               >  6:184363/1‑149 (MQ=255)
                                                                                                                          cc‑TGGACAAGGTGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAccc              <  3:235224/149‑1 (MQ=255)
                                                                                                                          cc‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCAccc              >  2:51607/1‑149 (MQ=255)
                                                                                                                                tccaGGCGGCTGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGt         <  5:57015/146‑1 (MQ=255)
                                                                                                                                aCAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGt         <  5:254368/149‑1 (MQ=255)
                                                                                                                                  aaGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGc       >  3:248969/1‑149 (MQ=255)
                                                                                                                                    ggCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGcc      >  6:5505/1‑148 (MQ=255)
                                                                                                                                    ggCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGcc      >  8:107672/1‑148 (MQ=255)
                                                                                                                                    ggCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGcc      >  8:206435/1‑148 (MQ=255)
                                                                                                                                    ggCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGcc      >  8:216980/1‑148 (MQ=255)
                                                                                                                                      cGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGCCaaa   <  1:30038/149‑1 (MQ=255)
                                                                                                                                       ggTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg                    <  4:73479/131‑1 (MQ=255)
                                                                                                                                       ggTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTgg                    >  3:73479/1‑131 (MQ=255)
                                                                                                                                       ggTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGCCAAAg  >  6:190995/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
GGCAGGGCGACACGGTATTGCAAGACCGCCTCAACCATGCCTCGTTGCTGGATGGCGGGTTGCTCAGTGGTGCCCGCTTCAACCGCTACCTGCACAACGACCCGGCCAGCCT‑GGCCAGCCGCC‑TGGACAAGGCGGTCGGCAATACCCTGGTGGTGACCGATGGCGTGTTCAGCATGGACGGTGACCTGGCCGACCTGCCGGCGCTGGCCAATGTCGCCCGTGCCCGCGGTGCCTGGCTGATGGTCGACGACGCCCATGGCCTGGGCACCCTGGGTGCCAAAG  >  NC_002947/441475‑441756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑