| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,827,647 | A→C | 10.9% | Q187P (CAG→CCG) | mtgA → | monofunctional biosynthetic peptidoglycan transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,827,647 | 0 | A | C | 10.9% | 154.2 / 9.2 | 55 | Q187P (CAG→CCG) | mtgA | monofunctional biosynthetic peptidoglycan transglycosylase |
| Reads supporting (aligned to +/- strand): ref base A (25/24); new base C (3/3); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CTGATCGAGCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCC > NC_002947/5827499‑5827786 | cTGATCGAGCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGgcagcgca < 3:197151/149‑1 (MQ=255) gAGCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCACCGCTgc > 1:41942/1‑147 (MQ=255) gAGCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCACCGCTgc > 7:234590/1‑147 (MQ=255) aGCTGTTCTGGTCGAAGGAACGGAATCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCa < 5:225755/149‑1 (MQ=255) gCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTgg < 7:162431/146‑1 (MQ=255) cTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtg < 6:96667/148‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgctgc < 3:12606/149‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgctg < 8:137926/148‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCACCGCTGCCAgctgcgctgc < 8:234590/149‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCACCGCTGCCAgctgcgctgc < 2:41942/149‑1 (MQ=255) aaGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAg > 3:210365/1‑149 (MQ=255) aaGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAg > 2:148263/1‑149 (MQ=255) aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc > 6:84861/1‑149 (MQ=255) aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc < 6:226359/149‑1 (MQ=255) aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc > 1:255622/1‑149 (MQ=255) ggAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGcc < 3:262648/149‑1 (MQ=255) aaCGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGccc > 2:105693/1‑148 (MQ=255) aCGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCaa > 4:113489/1‑149 (MQ=255) ttCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAg < 5:84861/149‑1 (MQ=255) tcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGTCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg > 7:178693/1‑148 (MQ=255) gTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGc < 3:113489/149‑1 (MQ=255) tCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGc > 3:289864/1‑148 (MQ=255) tATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag < 2:51944/127‑2 (MQ=255) tATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag > 1:51944/1‑126 (MQ=255) gAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg < 2:153888/135‑1 (MQ=255) gAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg > 1:153888/1‑135 (MQ=255) gAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGccgac > 7:200191/1‑149 (MQ=255) aaCAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGccgacc > 6:109396/1‑149 (MQ=255) gcCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGc < 3:274972/149‑1 (MQ=255) cGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGTCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCt < 8:178693/149‑1 (MQ=255) gCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGc > 8:224935/1‑148 (MQ=255) aGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAgc > 7:54258/1‑149 (MQ=255) gCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgg < 4:210365/149‑1 (MQ=255) ggTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTg > 8:146141/1‑149 (MQ=255) ccAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTc > 1:250746/1‑149 (MQ=255) aGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGc < 8:54258/149‑1 (MQ=255) aGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGCGCCGGCTGGATTCGc > 4:276186/1‑149 (MQ=255) gcggcgCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCa < 7:224935/149‑1 (MQ=255) gcgcGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGccgaccga < 4:29296/106‑1 (MQ=255) gcgcGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGccgaccga > 3:29296/1‑106 (MQ=255) gTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGc > 6:75855/1‑149 (MQ=255) aCTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCt < 7:146141/148‑1 (MQ=255) aCTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTg > 2:303222/1‑149 (MQ=255) ggctGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgg < 5:273085/146‑1 (MQ=255) gCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcgg < 2:250746/148‑1 (MQ=255) aCGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGc > 7:264220/1‑149 (MQ=255) aCGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGc < 3:140364/149‑1 (MQ=255) gCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTa > 8:140215/1‑149 (MQ=255) ccAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGGGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCCCGCCCAGCtgg < 1:148263/149‑3 (MQ=255) ccGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGa < 4:289864/149‑1 (MQ=255) tCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAg > 8:218636/1‑149 (MQ=255) ccgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTg > 3:148468/1‑148 (MQ=255) gcttggcagCACCGCTGCCAGCTGCGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcc > 5:27098/5‑149 (MQ=255) agggagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACtcctcc < 2:255622/149‑1 (MQ=255) ggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc > 3:3923/1‑147 (MQ=255) ggcagCACCGCTGCCAGCTGCGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACtcctcc < 6:27098/148‑1 (MQ=255) cgcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa > 2:159908/1‑117 (MQ=255) | CTGATCGAGCTGTTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGTCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGAGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCC > NC_002947/5827499‑5827786 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |