Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,843,495 T→C 8.1% G39G (GGT→GGC)  PP_5122 → GMC family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,843,4950TC 8.1% 132.7 / 2.1 49G39G (GGT→GGC) PP_5122GMC family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (25/20);  new base C (2/2);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CCGAAAATCTGAAAACAACAAGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTACCAGGAAGGGCTGGGGCG  >  NC_002947/5843347‑5843641
                                                                                                                                                    |                                                                                                                                                  
ccGAAAATCTGAACACAACAAGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAgtggt                                                                                                                                                    <  8:178097/149‑1 (MQ=255)
    aaaTCTGAAAACAACAAGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGc                                                                                                                                                  <  8:137751/147‑1 (MQ=255)
    aaaTCTGAAAACAACAAGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCcg                                                                                                                                                >  6:155864/1‑149 (MQ=255)
                  caaGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGt                                                                                                                                  >  6:138725/1‑149 (MQ=255)
                  caaGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGCGCCGCCGGcaccactg                                                                                                                                    >  4:59073/1‑145 (MQ=255)
                  caaGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGCGCCGCCGGcaccactg                                                                                                                                    <  3:59073/147‑3 (MQ=255)
                            gCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATg                                                                                                                         <  5:155864/148‑1 (MQ=255)
                              cAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTc                                                                                                                      <  1:53978/149‑1 (MQ=255)
                                         cccGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCgg                                                                                                           >  8:19707/1‑149 (MQ=255)
                                            gACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGttt                                                                                                        <  7:60524/149‑1 (MQ=255)
                                             aCCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGCGCCGCCGGCACCACCAGTGCGCAGATGCTCAGCGCTGCCGGttt                                                                                                        >  8:23548/1‑148 (MQ=255)
                                               ccGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTc                                                                                                       >  2:217588/1‑147 (MQ=255)
                                               ccGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTc                                                                                                       >  3:257973/1‑147 (MQ=255)
                                               ccGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCaa                                                                                                     <  7:126200/149‑1 (MQ=255)
                                                 gTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAgg                                                                                                   <  5:138725/149‑1 (MQ=255)
                                                  tttCCCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGc                                                                                                              >  8:79295/1‑137 (MQ=255)
                                                  tttCCCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGc                                                                                                              <  7:79295/137‑1 (MQ=255)
                                                    tCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTa                                                                                                 <  4:257973/148‑1 (MQ=255)
                                                      gCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTActg                                                                                              >  1:69309/1‑149 (MQ=255)
                                                       ccAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTActgc                                                                                             <  7:216432/149‑1 (MQ=255)
                                                        cAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTActgct                                                                                            >  1:232324/1‑149 (MQ=255)
                                                         aaGGCCTCGAACGCGGCTGGATCAACCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTActgct                                                                                            >  4:272943/1‑148 (MQ=255)
                                                          aGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGa                                                                                          <  3:83575/149‑1 (MQ=255)
                                                                     gcgGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGCGCCGCCGGCACCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGc                                                                               <  7:23548/149‑1 (MQ=255)
                                                                       ggCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGcc                                                                              >  2:104071/1‑148 (MQ=255)
                                                                         cTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCAc                                                                           <  7:19707/149‑1 (MQ=255)
                                                                           ggATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACt                                                                          >  6:140857/1‑148 (MQ=255)
                                                                             aTCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGaa                                                                       <  3:272943/149‑1 (MQ=255)
                                                                                  ccACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGAc                                                                    >  1:212945/1‑147 (MQ=255)
                                                                                  ccACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGAc                                                                    >  6:290146/1‑147 (MQ=255)
                                                                                      gacgCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACcagcag                                                              <  1:104071/149‑1 (MQ=255)
                                                                                         gCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGa                                                           >  6:259033/1‑149 (MQ=255)
                                                                                               cgcCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCa                                                     <  2:212945/149‑1 (MQ=255)
                                                                                                gcCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATTCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAATACCAGCAGCGATTTCCAc                                                    <  2:232324/149‑1 (MQ=255)
                                                                                                         aCGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCTGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGgag                                           <  5:290146/149‑1 (MQ=255)
                                                                                                          cGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGga                                            >  5:308577/1‑147 (MQ=255)
                                                                                                           gACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTACCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGagag                                         >  3:135539/1‑149 (MQ=255)
                                                                                                                gTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAg                                    >  2:257223/1‑149 (MQ=255)
                                                                                                                 tttCTGGATGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCGCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGc                                   <  8:158495/149‑1 (MQ=255)
                                                                                                                 tttCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGaa                                     <  5:259033/147‑1 (MQ=255)
                                                                                                                 tttCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGc                                   >  4:279421/1‑149 (MQ=255)
                                                                                                                    cTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCTACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCt                                 <  1:217588/148‑1 (MQ=255)
                                                                                                                    cTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTa                                >  6:37877/1‑149 (MQ=255)
                                                                                                                       gAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGc                             >  5:144205/1‑149 (MQ=255)
                                                                                                                            cGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGcc                        >  8:49931/1‑149 (MQ=255)
                                                                                                                             gACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCt                       >  8:195222/1‑149 (MQ=255)
                                                                                                                                aTTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTa                    >  5:231799/1‑149 (MQ=255)
                                                                                                                                aTTGCCGTGATCGGCAGTGGTGCCGGCGCCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTa                    >  1:175686/1‑149 (MQ=255)
                                                                                                                                    ccGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTACCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTACCa                 <  4:135539/148‑1 (MQ=255)
                                                                                                                                             gCAGTGGTGCCGGCGCCCCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTCCAGAACGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACATGCTGGAGAGCGAAGCCTACCCCAGCCTGTACCAGGAAGGGCTg       >  5:175069/1‑149 (MQ=255)
                                                                                                                                                   gtgCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTACCAGGAAGGGCTGGGgcg  <  6:144205/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCGAAAATCTGAAAACAACAAGAGCATCGCCAATGCCTGTCCCCGACCCGTTTCGCCAAGGCCTCGAACGCGGCTGGATCACCCACGACGCCTCGCGCCTGGAGCACGACCTGTTTCTGGAGGCCGACATTGCCGTGATCGGCAGTGGTGCCGGCGGCGCCACCAGTGCGCAGATGCTCAGCGCTGCCGGTTTCAAGGTACTGCTGATCGAAGAAGGCCCACTGAAGACCAGCAGCGATTTCCACCTGCTGGAGAGCGAAGCCTACGCCAGCCTGTACCAGGAAGGGCTGGGGCG  >  NC_002947/5843347‑5843641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑