| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 445,088 | C→G | 13.8% | intergenic (+61/‑227) | PP_0367 → / → PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 445,088 | 0 | C | G | 13.8% | 91.3 / 2.9 | 35 | intergenic (+61/‑227) | PP_0367/PP_0368 | hypothetical protein/acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (10/20); new base G (2/3); total (12/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAA > NC_002947/444943‑445235 | gggCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTc < 4:1871/149‑1 (MQ=255) ggCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTc < 3:302343/148‑1 (MQ=255) ggCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTc < 7:276988/148‑1 (MQ=255) ggCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATGTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTcc > 8:136940/1‑149 (MQ=255) aaGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGAt < 7:271649/149‑1 (MQ=255) cGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGac < 6:6308/149‑1 (MQ=255) cGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGac < 2:65798/149‑1 (MQ=255) aaGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGacaa < 6:3740/149‑1 (MQ=255) gCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAAtttt < 1:158333/149‑1 (MQ=255) tCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACGACTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACAAAATtgttgt > 1:234724/1‑149 (MQ=25) aaaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCg < 7:136940/149‑1 (MQ=255) aaaGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCg < 5:90189/149‑1 (MQ=255) tcAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCGGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCctct > 1:66905/1‑148 (MQ=255) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCgg < 1:146750/149‑1 (MQ=255) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCgg < 1:109535/149‑1 (MQ=18) gCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGCGCATTCCCCCG‑AATGCGTCCGATCACACAACAATTTTGTCTGGTAGGGCCACTTCCCgg > 8:236362/1‑149 (MQ=255) cATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AAGAGGCCCGACCAGACAAAATTGTTGTCTGATCGGACGCATTCGCggg < 2:234724/149‑1 (MQ=18) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc > 3:68573/1‑137 (MQ=255) aTCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGc < 4:68573/137‑1 (MQ=255) cgacgaAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑CATTGGTCCGATAAGGCAACAAATTTTTATGGTGGGGCCGCTTAGCGGGGaat > 8:86817/1‑148 (MQ=255) ggATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGGATGACCCCGCAATGC‑TCCGAAAAAACAAAAAATTTTTTAGGTGGGGGCGAATAGCGGGCAACCCCCCACCAACAATGGt > 8:281723/1‑149 (MQ=255) ccTACGCCTTAATACTTGCGTAATCCCTTATTTAAGCGCCTGCGTTGTAGGTGCGGGTTTGCCCGCT‑AATGCGTCCGATCAGACAAAAATTTTGTCTGGTCGGGCCTCTTCGCGGGCATGCCCGCTCCTACAAGGGTTTGtggcatggc < 6:280801/149‑1 (MQ=255) tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTTCCCGCG‑AATTCGTCCCATCAGACAACAATTTTTTGTGGTCGGGCCTCATAGCGGGCAAGCCCCCTc > 8:282785/1‑119 (MQ=255) tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAAtt < 2:180033/83‑1 (MQ=255) tGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAAtt > 1:180033/1‑83 (MQ=255) tGAATACTTGCGTCAGCCCCTATTTCCGCGCCTGCCTTGTCGGAGCGGGCTTGCCCGCT‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTc < 7:282785/119‑1 (MQ=38) aTACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGGTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt < 7:281723/149‑1 (MQ=255) aTACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCT‑AATGCGTCCGATCACACAACAATTTTGTCTGGGCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGAttt < 7:86817/149‑1 (MQ=255) tgcctTGTAGGAGCGGGCTTGCCCCCG‑AATGCGTCCGATCAGACAACAATTTTTTGTGGTCGGGCCTCTTAGCGGGCCAGCCCGCTCCTACAAGGGGTTGTGGGATGGCATGACTGATTTTCCATTTTTAGTGGGGTAAATGGCACCa > 6:309129/1‑148 (MQ=37) ggtgggggCTTGCCCGCG‑AATGGGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTGTTCGCGGGCTAGCCCGCTGCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATc < 7:236362/143‑1 (MQ=255) gCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGa > 2:188809/1‑146 (MQ=255) cccGCT‑AATGCGTCCGATCAGACAACAATTTTGGCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGc < 5:309129/149‑1 (MQ=255) ccGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAAt < 4:37880/119‑1 (MQ=255) cgcg‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTg < 1:188809/149‑1 (MQ=255) gCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTaaa > 4:301231/1‑149 (MQ=255) | GGGCAAGACCGAAGTCGAGCTCGGCGCAAAGGTCGTCAAGGCATCCGACGAAATGCTTGGCCGGATCATCGACACCTACGCCTGAATACTTGCGTCATCCCCTATTTCAGCGCCTGCCTTGTAGGAGCGGGCTTGCCCGCG‑AATGCGTCCGATCAGACAACAATTTTGTCTGGTCGGGCCTCTTCGCGGGCAAGCCCGCTCCTACAAGGGTTTGTGGCATGGCATGACTGATTTGCCATTTTTCGTGGCGTAAATGGCACCATAGTCCATCCTTGACAGAAAGCTGAGCTAAA > NC_002947/444943‑445235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |