Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,845,972	T→C	9.1%	intergenic (+44/+47)	yfhL → / ← mutM	4Fe‑4S cluster‑containing protein/formamidopyrimidine‑DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,845,972	0	T	C	9.1%	182.6 / 7.2	66	intergenic (+44/+47)	yfhL/mutM	4Fe‑4S cluster‑containing protein/formamidopyrimidine‑DNA glycosylase
Reads supporting (aligned to +/- strand):  ref base T (32/28);  new base C (3/3);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCC  >  NC_002947/5845829‑5846107                                                                                                                                                  |                                                                                                                                        gcagGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTcc                                                                                                                                    <  2:280984/149‑1 (MQ=255)   agGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCca                                                                                                                                  <  1:158744/149‑1 (MQ=255)     gTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGGGAGCAGGCATGCCCGCTCTca                                                                                                                                  >  5:196230/1‑147 (MQ=255)     gTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGCGCGGGCATGCCTGCTcccc                                                                                                                                  >  5:10304/1‑146 (MQ=255)     gTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCca                                                                                                                                  >  2:298168/1‑147 (MQ=255)        tGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATAATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAggt                                                                                                                             >  6:7699/1‑147 (MQ=255)          cccGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGGGAGCAGGCATGCCCGCTCTCACAgggac                                                                                                                           <  6:196230/149‑5 (MQ=255)          cccGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTa                                                                                                                           <  4:287237/149‑1 (MQ=255)                 gACTGCATCCCGCTGGATGAAGCGCATCCGTAAACCGAAGAACAGCTGAGGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGttt                                                                                                                            <  8:195789/141‑1 (MQ=255)                 gACTGAATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCAGGAT‑‑CACTGGCAAGCGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGttt                                                                                                                            >  7:195789/1‑141 (MQ=255)                  aCTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGATTGTGCTGGAAAGGGCTGATCATTGCCCTACCCTGAACGGTCCCTGTGAGAGCGGGAATCCCTGATCCCACAGTTTAGGTTTc                                                                                                                     >  1:249697/1‑149 (MQ=255)                       aTCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCaa                                                                                                              <  2:89420/149‑1 (MQ=255)                             ctcgATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGtt                                                                                                        <  5:285405/146‑1 (MQ=255)                                 aTGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCa                                                                                                    <  8:291856/149‑1 (MQ=255)                                  tGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAg                                                                                                   >  2:35855/1‑149 (MQ=255)                                   gAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGc                                                                                                  >  2:162547/1‑149 (MQ=255)                                      gcgcATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGaa                                                                                               <  2:249697/149‑1 (MQ=255)                                              ggAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCaa                                                                                                              <  5:258217/126‑1 (MQ=255)                                              ggAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCaa                                                                                                              >  6:258217/1‑126 (MQ=255)                                              ggAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCAt                                                                                       >  5:49963/1‑149 (MQ=255)                                                    cGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg                                                                                 >  6:131073/1‑149 (MQ=255)                                                    cGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg                                                                                 <  6:49963/149‑1 (MQ=255)                                                          aCAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAgcgc                                                                           <  1:162547/149‑1 (MQ=255)                                                           cAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGcgcg                                                                          >  7:3018/1‑149 (MQ=255)                                                             gCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGAAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCggg                                                                        <  8:4983/149‑1 (MQ=255)                                                                 aTGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAAc                                                                                                             <  4:260470/108‑1 (MQ=255)                                                                 aTGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAAc                                                                                                             >  3:260470/1‑108 (MQ=255)                                                                 aTGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGt                                                                    <  8:3018/149‑1 (MQ=255)                                                                     ccAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTc                                                                                                       <  6:311614/110‑1 (MQ=255)                                                                     ccAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTc                                                                                                       >  5:311614/1‑110 (MQ=255)                                                                       aaGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGc                                                              >  5:177584/1‑149 (MQ=255)                                                                          tATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGATCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCg                                                           >  7:150214/1‑149 (MQ=255)                                                                              gCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCct                                                       >  1:47431/1‑149 (MQ=255)                                                                                    t‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGccc                                                 >  4:46634/1‑149 (MQ=255)                                                                                         cTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAg                                              <  7:128872/149‑1 (MQ=255)                                                                                         cTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGGTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAg                                              >  5:251104/1‑149 (MQ=255)                                                                                            gCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTc                                                                                         <  3:91525/103‑1 (MQ=255)                                                                                            gCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTc                                                                                         >  4:91525/1‑103 (MQ=255)                                                                                            gCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCtt                                           >  6:148908/1‑149 (MQ=255)                                                                                                    tGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCACCCTCTGCCCCAGCTTCACTTcgc                                   >  1:43220/1‑149 (MQ=255)                                                                                                      aTCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg                                                                                 <  5:27378/101‑1 (MQ=255)                                                                                                      aTCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTgg                                                                                 >  6:27378/1‑101 (MQ=255)                                                                                                         aTTGCGCTTCCCTGCACGGTCCCTGTGAGAGCGGGGATGCCTGGTCGCACAGTTTAGGTTTCGCTTCTACAGGTTCGCAGCGAACGTTTGATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGAAAc                              <  6:251104/149‑1 (MQ=255)                                                                                                          ttGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACt                             >  8:168105/1‑149 (MQ=255)                                                                                                             cccTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCa                           <  6:177584/148‑1 (MQ=255)                                                                                                             cccTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGCTTCGCTTCAACAGGTTCGCAGCTAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCa                           <  5:131073/148‑1 (MQ=255)                                                                                                              ccTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGt                         <  3:46634/149‑1 (MQ=255)                                                                                                                ttCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTcgc                                   >  4:104103/1‑137 (MQ=255)                                                                                                                ttCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTcgc                                   <  3:104103/137‑1 (MQ=255)                                                                                                                ttCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGc                       >  8:15935/1‑149 (MQ=255)                                                                                                                  cccTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCg                     >  4:48815/1‑149 (MQ=255)                                                                                                                   ccTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCg                     <  1:298168/148‑1 (MQ=255)                                                                                                                    cTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCACCTCAGTGCCGCa                   >  6:230607/1‑149 (MQ=255)                                                                                                                     tGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCAc                                        <  7:131073/127‑1 (MQ=255)                                                                                                                     tGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCAc                                        >  8:131073/1‑127 (MQ=255)                                                                                                                        aCGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATAcc               <  3:48815/149‑1 (MQ=255)                                                                                                                             aacTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGc           >  5:235886/3‑148 (MQ=255)                                                                                                                             aacTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGc           <  6:235886/146‑1 (MQ=255)                                                                                                                              ccTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCAc         >  5:247611/1‑149 (MQ=255)                                                                                                                              acTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCa                   >  2:167522/2‑139 (MQ=255)                                                                                                                              acTGTGGGAGCAGGCATGCCCGCTCTCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCa                   <  1:167522/138‑1 (MQ=255)                                                                                                                               cTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCatg        >  8:64392/1‑147 (MQ=255)                                                                                                                                tgtgAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACgg       >  7:21776/1‑149 (MQ=255)                                                                                                                                    agagCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGc   <  6:10304/149‑1 (MQ=255)                                                                                                                                      agCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGcc  >  3:90133/1‑148 (MQ=255)                                                                                                                                      agCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGcc  <  4:90133/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                        GCAGGTCTGCCCGGTCGACTGCATCCCGCTGGATGAAGCGCATCCGGAAACCGAAGAACAGCTGATGGCCAAGTATCGCCGGAT‑‑CACTGGCAAGGGCTGATCATTGCCCTTCCCTGCACGGTCCCTGTGAGAGCGGGCATGCCTGCTCCCACAGTTTAGGTTTCGCTTCAACAGGTTCGCAGCGAACGTTTCATCGTTGGCAGCGCGGGCAGTACACGCTCGCCCTCTGCCCCAGCTTCACTTCGCGCAACTCAGTGCCGCATACCTTGCACGGCTGCC  >  NC_002947/5845829‑5846107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑