Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,882,049 G→A 7.8% A204T (GCT→ACT)  serA → bifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,882,0490GA 7.8% 145.7 / 2.3 51A204T (GCT→ACT) serAbifunctional D‑3‑phosphoglycerate dehydrogenase/alpha‑ketoglutarate reductase
Reads supporting (aligned to +/- strand):  ref base G (28/19);  new base A (2/2);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

GGCATCGTCGGCTACGGCTCGATTGGTACCCAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCT  >  NC_002947/5881902‑5882192
                                                                                                                                                   |                                                                                                                                               
ggCATCGTCGGCTACGGCTCGATTGGTACCCAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTgg                                                                                                                                                 <  4:247265/148‑1 (MQ=255)
  cATCGTCGGCTACGGCTCGATTGGTACCCAGCTTTAGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCt                                                                                                                                               >  8:70869/1‑148 (MQ=255)
                 cTCGATTGGTACCCAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGTAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTg                                                                                                                                >  5:35806/1‑148 (MQ=255)
                            cccAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAg                                                                                                                    >  1:12018/1‑149 (MQ=255)
                             ccAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGc                                                                                                                   >  3:49692/1‑149 (MQ=255)
                                  tttCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCt                                                                                                                       <  6:241825/140‑1 (MQ=255)
                                  tttCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCt                                                                                                                       >  5:241825/1‑140 (MQ=255)
                                  tttCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGACTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCa                                                                                                              >  6:112909/1‑149 (MQ=255)
                                             gCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGt                                                                                                    <  1:36058/148‑1 (MQ=255)
                                              cTGAGAACATGGTCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTgg                                                                                                  <  3:100987/149‑1 (MQ=255)
                                                gagaACATGGGCATGCAGGTGTTCTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgat                                                                                                >  7:194770/1‑149 (MQ=255)
                                                 agaACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGga                                                                                                 <  1:37070/147‑1 (MQ=255)
                                                  gaACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatgg                                                                                              <  8:194770/149‑2 (MQ=255)
                                                   aaCATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGTCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGc                                                                                                                         <  6:235033/121‑1 (MQ=255)
                                                   aaCATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGTCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGc                                                                                                                         >  5:235033/1‑121 (MQ=255)
                                                    aCATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatggca                                                                                            <  7:70869/149‑4 (MQ=255)
                                                    aCATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATc                                                                                            <  8:221312/149‑1 (MQ=255)
                                                             tGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAg                                                                                   <  3:128197/149‑1 (MQ=255)
                                                                aGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGaa                                                                                <  2:12018/149‑1 (MQ=255)
                                                                aGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGa                                                                                 >  1:297648/1‑148 (MQ=255)
                                                                         tcttcGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTc                                                                        >  3:241262/1‑148 (MQ=255)
                                                                                 ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatggca                                                                                            >  4:52227/1‑117 (MQ=255)
                                                                                 ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACTGGGTGgatggca                                                                                            <  3:52227/120‑4 (MQ=255)
                                                                                 ccGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGACTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATgaagaa                                                               <  5:112909/149‑1 (MQ=255)
                                                                                  cGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGaagaag                                                              >  6:9999/1‑149 (MQ=255)
                                                                                         cAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGa                                                       >  6:130666/1‑149 (MQ=255)
                                                                                          aaGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGag                                                      >  8:252730/1‑148 (MQ=255)
                                                                                           aGCTGCCACTGGGCAACGCCGTGCAGGACACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGCAGGGCGCGATc                                                     >  1:9077/1‑149 (MQ=255)
                                                                                              tGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGATATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCt                                                   >  5:129905/1‑148 (MQ=255)
                                                                                                ccACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGAt                                                >  1:272602/1‑149 (MQ=255)
                                                                                                  aCTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATc                                               >  3:19044/1‑148 (MQ=255)
                                                                                                    tGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAAc                                            >  4:84107/1‑149 (MQ=255)
                                                                                                      ggCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAAc                                            <  2:297648/147‑1 (MQ=255)
                                                                                                        cAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAAcgccg                                        >  5:98976/1‑149 (MQ=255)
                                                                                                         aaCGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAAcgccgc                                       >  3:263492/1‑149 (MQ=255)
                                                                                                               gTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCGACGCCGCCCGTgg                                 >  4:34335/1‑149 (MQ=255)
                                                                                                                   aGGTCACCAGCCTGAACGAGCTGCTGGGCCTGACTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAgg                                                             >  5:191336/1‑117 (MQ=255)
                                                                                                                   aGGTCACCAGCCTGAACGAGCTGCTGGGCCTGACTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAgg                                                             <  6:191336/117‑1 (MQ=255)
                                                                                                                         ccAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTc                       <  4:19044/149‑1 (MQ=255)
                                                                                                                         ccAGCCTGAACGAGCTGCTCGGCCTGGCTGACATCGTCTCGCTGCACGTGCATGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGAGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTc                       >  8:216895/1‑149 (MQ=255)
                                                                                                                              cTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCt                  >  6:92870/1‑149 (MQ=255)
                                                                                                                                  aCGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGac              <  6:35806/149‑1 (MQ=255)
                                                                                                                                  aCGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGac              <  3:84107/149‑1 (MQ=255)
                                                                                                                                      gctgctGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGgcgc                                                         >  5:263355/1‑102 (MQ=255)
                                                                                                                                      gctgctGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGgcgc                                                         <  6:263355/102‑1 (MQ=255)
                                                                                                                                         gctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTgg       >  1:197250/1‑149 (MQ=255)
                                                                                                                                         gctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTgg       >  4:106735/1‑149 (MQ=255)
                                                                                                                                          ctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGc      <  2:272602/149‑1 (MQ=255)
                                                                                                                                          ctgGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCCAGCTCGaccacggt        <  5:130666/147‑4 (MQ=255)
                                                                                                                                              gCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGcgcg                                                        >  6:209114/1‑95 (MQ=255)
                                                                                                                                              gCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGcgcg                                                        <  5:209114/95‑1 (MQ=255)
                                                                                                                                              gCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCt  >  6:193473/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGCATCGTCGGCTACGGCTCGATTGGTACCCAGCTTTCGGTACTGGCTGAGAACATGGGCATGCAGGTGTACTTCTTCGACCCGCTGACCAAGCTGCCACTGGGCAACGCCGTGCAGGTCACCAGCCTGAACGAGCTGCTGGGCCTGGCTGACATCGTCTCGCTGCACGTGCCTGAGCTGCCATCCACCCAGTGGATGATCGGCGAGAAGGAAATCCGCTCGATGAAGAAGGGCGCGATCCTGATCAACGCCGCCCGTGGCACCGTGGTCGAGCTGGACCACCTGGCCGCT  >  NC_002947/5881902‑5882192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑