Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,890,791 A→C 9.1% intergenic (+79/+41) PP_5164 → / ← plpB penicillin amidase family protein/NLPA lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,890,7910AC 9.1% 123.2 / 4.7 44intergenic (+79/+41)PP_5164/plpBpenicillin amidase family protein/NLPA lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (23/17);  new base C (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTT  >  NC_002947/5890646‑5890939
                                                                                                                                                 |                                                                                                                                                    
gTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAgg                                                                                                                                                   >  1:5344/1‑149 (MQ=255)
   tCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                    <  4:76689/145‑1 (MQ=255)
   tCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAg                                                                                                                                                    >  3:76689/1‑145 (MQ=255)
     aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACCTTGTGTCg                                                                                                                                              >  2:5738/1‑149 (MQ=255)
     aGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTc                                                                                                                                               <  7:126209/148‑1 (MQ=255)
      gCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCgg                                                                                                                                             >  2:255079/1‑149 (MQ=255)
       cGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACCTTGTGTCGGc                                                                                                                                            <  1:5738/149‑1 (MQ=255)
          gcgcAGATGGGGGTTATTCCGGCGCAAAGTACCTTACGGCTGATACCCCCGAAGAAAGAGGCTGCATTGCGGCCTATTCGCAGCAAAAGGAATCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGACCACACAAGGTTTCGGCGtt                                                                                                                                         >  7:45540/1‑149 (MQ=255)
          gcgcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGtt                                                                                                                                         >  2:281780/1‑149 (MQ=255)
           cgcAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACCTTGTGTCGGCGttt                                                                                                                                        <  8:24885/149‑2 (MQ=255)
                aTGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGcaca                                                                                                                                   >  8:39646/1‑149 (MQ=255)
                      gTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAg                                                                                                                             >  8:288013/1‑149 (MQ=255)
                            ccGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGtt                                                                                                                        <  2:199915/148‑1 (MQ=255)
                                cgcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAg                                                                                                                   >  6:187977/1‑149 (MQ=255)
                                 gcAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGc                                                                                                                  <  7:288013/149‑1 (MQ=255)
                                               ggCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGcc                                                                                                    >  4:40214/1‑149 (MQ=255)
                                                    gTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCggta                                                                                                <  2:5344/148‑3 (MQ=255)
                                                                  agagGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTgg                                                                                  >  2:156259/1‑148 (MQ=255)
                                                                   gagGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTa                                                                                >  2:92057/1‑149 (MQ=255)
                                                                      gctgctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActt                                                                             >  1:125297/1‑149 (MQ=255)
                                                                          ctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtctt                                                                          <  1:255079/148‑1 (MQ=255)
                                                                          ctTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTg                                                                         <  3:184364/149‑1 (MQ=255)
                                                                           tttGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtctt                                                                          >  8:166864/1‑147 (MQ=255)
                                                                           tttGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTActtctt                                                                          <  7:166864/147‑1 (MQ=255)
                                                                             tGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTgg                                                                        <  5:187977/147‑1 (MQ=255)
                                                                                  ccGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCaatgaa                                                                 >  5:130388/1‑149 (MQ=255)
                                                                                      ttCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGAGTTGCAAGCGAAATCAGAACGCCGGCAGTACGGCACCATGGTACTTCTTgg                                                                        >  2:259655/1‑138 (MQ=255)
                                                                                        cGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGcc                                                             <  8:209184/147‑1 (MQ=255)
                                                                                         gcagcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCtt                                                           >  1:77710/1‑148 (MQ=255)
                                                                                          cagcaAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGa                                                         >  6:4717/1‑149 (MQ=255)
                                                                                                aaGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCg                                                   <  1:156259/149‑1 (MQ=255)
                                                                                                  ggCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                 <  3:40214/149‑1 (MQ=255)
                                                                                                  ggCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCggg                                                 >  8:129273/1‑149 (MQ=255)
                                                                                                        tACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGt                                            >  7:236837/1‑148 (MQ=255)
                                                                                                                       gCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGc                                                <  3:4849/129‑1 (MQ=255)
                                                                                                                       gCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGc                                                >  4:4849/1‑129 (MQ=255)
                                                                                                                                aagaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGAt                    <  8:236837/148‑1 (MQ=255)
                                                                                                                                  gaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGc                 >  8:23709/1‑149 (MQ=255)
                                                                                                                                  gaaAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGc                 <  1:92057/149‑1 (MQ=255)
                                                                                                                                      aaCGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa             <  7:129273/149‑1 (MQ=255)
                                                                                                                                       aCGCCGAAACAAGGTGTCGGCGTTGGCACATATAAGGCGTTGCTAGCGAAATCAGAACGCCGGCACTACGGCACCTTGGTACTACTTGGCAATGAATGCCTTGACTTCCGCGCTGGTCAGGGCCTTGGCCAGTTTCTGTCTGTCCTCa             >  8:278450/1‑148 (MQ=255)
                                                                                                                                         gCCGACACCTTGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTg          >  6:294941/1‑149 (MQ=255)
                                                                                                                                             acacAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa             <  6:23008/142‑1 (MQ=255)
                                                                                                                                             acacAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCa             >  5:23008/1‑142 (MQ=255)
                                                                                                                                              cacaAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCtt     <  5:4717/149‑1 (MQ=255)
                                                                                                                                                 aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgtt  >  3:132046/1‑149 (MQ=255)
                                                                                                                                                 aaGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCttgtt  <  7:23709/149‑1 (MQ=255)
                                                                                                                                                 aaGGTGTCGGCGTTAGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGTTGGTCAGGGCCTTGGCCAGTTTCTGGATg                   <  3:124470/132‑1 (MQ=255)
                                                                                                                                                 aaGGTGTCGGCGTTAGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGTTGGTCAGGGCCTTGGCCAGTTTCTGGATg                   >  4:124470/1‑132 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTATCAGCGGGCGCAGATGGGGGTGATTCCGGCGCAAAGTACCTTACGGCTGGTACCCCCGAAGTAAGAGGCTGCTTTGCGGCCGCTTCGCAGCAAAAGGCTTCTACGGTGCTGTGCGGGCAGGGCTGAAGAAAAACGCCGACACAAGGTGTCGGCGTTGGCACATATCAGGCGTTGCTAGCGAAATCAGAACGCCGGCAGTACGGCACCTTGGTACTTCTTGGCAATGAATGCCTTGACTTCCGGGCTGGTCAGGGCCTTGGCCAGTTTCTGGATGGCCTCACTGTCCTTGTT  >  NC_002947/5890646‑5890939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑