Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,919,639	G→T	7.3%	K75N (AAG→AAT)	PP_5190 →	type II secretion system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,919,639	0	G	T	7.3%	172.4 / 3.8	55	K75N (AAG→AAT)	PP_5190	type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base T (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.84e-01

ATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTT  >  NC_002947/5919497‑5919787                                                                                                                                                    |                                                                                                                                                     aTACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGc                                                                                                                                                <  4:128341/149‑1 (MQ=255)     ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAAc                                                                                                                                            >  3:193146/1‑149 (MQ=255)     ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAAc                                                                                                                                            <  3:77743/149‑1 (MQ=255)     ccTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAAc                                                                                                                                            <  4:139566/149‑1 (MQ=255)      cTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAAcc                                                                                                                                           <  5:6053/149‑1 (MQ=255)          aaGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTAc                                                                                                                                       <  7:256767/149‑1 (MQ=255)               cTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTgcg                                                                                                                                  >  5:285397/1‑149 (MQ=255)                      acGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGAc                                                                                                                           <  5:68588/149‑1 (MQ=255)                          cgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCt                                                                                                                                     >  4:81212/1‑135 (MQ=255)                          cgcTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCt                                                                                                                                     <  3:81212/135‑1 (MQ=255)                                ccACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAAc                                                                                                                 >  2:206101/1‑149 (MQ=255)                                 cACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCTAGGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGTTGGCCGAC‑‑C‑TTGTCGGCCAGcc                                                                                                                                           >  1:218990/1‑123 (MQ=255)                                 cACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGTTGGCCGAC‑‑C‑TTGTCGGCCAGcc                                                                                                                                           <  2:218990/122‑1 (MQ=255)                                 cACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACt                                                                                                                >  3:49881/1‑149 (MQ=255)                                  aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGc                                                                                                                    >  1:289406/1‑144 (MQ=255)                                  aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGc                                                                                                                    <  2:289406/144‑1 (MQ=255)                                  aCCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCATGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTc                                                                                                               >  8:88204/1‑149 (MQ=255)                                     ccGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCATGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAc                                                                                                            <  7:88204/149‑1 (MQ=255)                                           gCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                        <  6:285397/147‑1 (MQ=255)                                           gCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                        <  5:72630/147‑1 (MQ=255)                                           gCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGc                                                                                                        <  4:193146/147‑1 (MQ=255)                                             cACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCAACCC‑GGCCAGCCCCTGGACTTGGAGCAACTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTAGGCCAACCCTACCTGCGCATAGACCCCATGCAACTCGCCCTGccc                                                                                                      >  7:244654/1‑147 (MQ=255)                                                            aTCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGAt                                                                                     >  1:174850/1‑149 (MQ=255)                                                                 cgccTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCCTGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTccc                                                                                <  7:125810/149‑1 (MQ=255)                                                                 cgccTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCa                                                                                            <  5:88791/137‑1 (MQ=255)                                                                 cgccTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCa                                                                                            >  6:88791/1‑137 (MQ=255)                                                                   ccTGCAGCCT‑GGAAAGCCGCCAGCACCCCGGGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTccc                                                                                >  1:22012/1‑148 (MQ=255)                                                                       cAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGTC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCt                                                                                        <  8:42186/135‑1 (MQ=255)                                                                       cAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCa                                                                              <  7:244510/145‑1 (MQ=255)                                                                       cAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCCACCTGCCCCAGGTCACCAGCCTGATCTCCCCa                                                                              >  8:244510/1‑145 (MQ=255)                                                                        aGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCATGTCACCAGcc                                                                                         <  3:64948/133‑1 (MQ=255)                                                                        aGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGcc                                                                                         >  4:64948/1‑133 (MQ=255)                                                                         gCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTc                                                                        <  4:49881/149‑1 (MQ=255)                                                                          ccT‑GGAAAGCCGCCAGGACCCC‑GGCCAGCCCCTTGACTGTGAGAACCTTTGCCAGTGGCTGGCCGAC‑‑A‑ATGTCGGCCAACCCTACCTGCGCATCGACCCCATTCAACTCGACCTGCCCCAGGTCACCATCCTGATCTCCCCAGCCTTCg                                                                       <  8:244654/149‑1 (MQ=255)                                                                          ccT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCGCCAGGTCACCAGCCTGATCTCCCCAGCCTTCg                                                                       <  6:284952/149‑1 (MQ=255)                                                                            t‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGcc                                                                     >  8:85805/1‑149 (MQ=255)                                                                                            aCCCC‑GGCCAGCCCCTGGCCTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGAGCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCAGGGCATc                                                      <  6:156777/149‑1 (MQ=255)                                                                                              ccc‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATcc                                                     >  4:192174/1‑148 (MQ=255)                                                                                              ccc‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCt                                                    >  3:172628/1‑149 (MQ=255)                                                                                              ccc‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCt                                                    >  1:232052/1‑149 (MQ=255)                                                                                               cc‑GGCCAGCCCCTGGTCTTGGAGCACCTTTGCCATTGGGTGGGCGACTTACAGTTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATc                                                      <  1:98770/149‑1 (MQ=255)                                                                                                c‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCa                                                  >  7:172913/1‑149 (MQ=255)                                                                                                        ccccTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTcgc                                           >  4:252608/1‑149 (MQ=255)                                                                                                          ccTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCgg                                         >  3:206030/1‑149 (MQ=255)                                                                                                           cTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGc                                        <  1:206101/149‑1 (MQ=255)                                                                                                                    gAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTc                               <  7:85805/149‑1 (MQ=255)                                                                                                                         ccTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGACCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCg                          >  1:21356/1‑149 (MQ=255)                                                                                                                               ccAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCAcc                     <  2:232052/148‑1 (MQ=255)                                                                                                                                cAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGt                   >  1:18335/1‑149 (MQ=255)                                                                                                                                 aGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTc                  <  2:22012/149‑1 (MQ=255)                                                                                                                                     gctggcCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                    >  7:52821/1‑127 (MQ=255)                                                                                                                                     gctggcCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGAt                                    <  8:52821/127‑1 (MQ=255)                                                                                                                                      ttggcCGAC‑‑C‑TTGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAg             >  2:106826/2‑149 (MQ=255)                                                                                                                                          ccGAC‑‑A‑AGGTCGGCCAACCCTACATGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGCCATCCTCATCGTCCCGGCCGAAGCCTCCAGCGTCACCGACGCCAGcccc         >  8:119675/1‑149 (MQ=255)                                                                                                                                           cGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGccc        <  2:18335/149‑1 (MQ=255)                                                                                                                                                    ggTCGGCCAACCCTACCTGCGCATCGCCCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCtt  >  8:67471/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     ATACCCTGCAAGTACTCGAACACGCCGCTGCCCACCCCGCTAGCCACCCGCTGGAGCACATCGCCGCCTGCAGCCT‑GGAAAGCCGCCAGCACCCC‑GGCCAGCCCCTGGACTTGGAGCACCTTTGCCAGTGGCTGGCCGAC‑‑A‑AGGTCGGCCAACCCTACCTGCGCATCGACCCCATGCAACTCGACCTGCCCCAGGTCACCAGCCTGATCTCCCCAGCCTTCGCCCAGCGCCACGGCATCCTCATCGTCGCGGCCGATGCCTCCAGCGTCACCGTCGCCAGCGCCCAGCCTT  >  NC_002947/5919497‑5919787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑