Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,934,695 A→T 9.1% noncoding (30/180 nt) PP_mr61 → 6S ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,934,6950AT 9.1% 124.6 / 3.6 44noncoding (30/180 nt)PP_mr616S ncRNA
Reads supporting (aligned to +/- strand):  ref base A (20/20);  new base T (2/2);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGACCGCAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACTTTCGGGTTCAAGGGCTACACCGATAGCG  >  NC_002947/5934548‑5934837
                                                                                                                                                   |                                                                                                                                              
aaGACCGCAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAg                                                                                                                                               >  2:57791/1‑149 (MQ=255)
     cGCAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGt                                                                                                                                          >  3:298346/1‑149 (MQ=255)
      gCAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGtt                                                                                                                                         <  2:232886/149‑1 (MQ=255)
       cAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTa                                                                                                                                        <  6:60538/149‑1 (MQ=255)
       cAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTa                                                                                                                                        >  5:60538/1‑149 (MQ=255)
          cGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGt                                                                                                                                     >  8:103152/1‑149 (MQ=255)
                  cGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGc                                                                                                                             >  7:99883/1‑149 (MQ=255)
                       aCCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCTGTCGGTTATGTCCCTGAGCCGATa                                                                                                                        >  8:75204/1‑149 (MQ=255)
                       aCCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCTGTCGGTTATGTCCCTGAGCCGATa                                                                                                                        >  8:70859/1‑149 (MQ=255)
                         cGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACg                                                                                                                      <  7:103152/149‑1 (MQ=255)
                          ggCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGc                                                                                                                     <  8:99883/149‑1 (MQ=255)
                           gCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGca                                                                                                                    <  8:94049/149‑1 (MQ=255)
                           gCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGca                                                                                                                    >  6:128737/1‑149 (MQ=255)
                            cACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGca                                                                                                                    >  6:222115/1‑148 (MQ=255)
                                   cgcgAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACg                                                                                                            >  1:205043/1‑149 (MQ=255)
                                    gcgAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACgg                                                                                                           <  1:57791/149‑1 (MQ=255)
                                     cgAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACggg                                                                                                          >  4:239959/1‑149 (MQ=255)
                                               cgcgACCTGCTGGATCGGGTGGATAAGGCTTTGTCAGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGc                                                                                                >  2:9646/1‑149 (MQ=255)
                                                      tgctgGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGcc                                                                                         >  4:189582/1‑149 (MQ=255)
                                                        ctgGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCgg                                                                                       <  5:222115/149‑1 (MQ=255)
                                                        ctgGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCg                                                                                        <  4:44316/148‑1 (MQ=255)
                                                             tCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGc                                                                                  <  3:238731/149‑1 (MQ=255)
                                                                ggTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCAt                                                                                >  8:110406/1‑148 (MQ=255)
                                                                ggTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCAt                                                                                <  7:110406/148‑1 (MQ=255)
                                                                ggTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATg                                                                               >  4:266901/1‑149 (MQ=255)
                                                                           cTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACg                                                                    <  4:298346/149‑1 (MQ=255)
                                                                              tGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACgg                                                                   <  3:189582/147‑1 (MQ=255)
                                                                                       acacGGATACCAAAATCGGCTGAGATTGGTATACTGTCGCCACTCCCTGGGGGATGCGCCTGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAAc                                                        <  7:70859/149‑1 (MQ=255)
                                                                                       acacGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCTGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAAc                                                        <  7:75204/149‑1 (MQ=255)
                                                                                       acacGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAAc                                                        <  5:128737/149‑1 (MQ=255)
                                                                                       acacGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAAc                                                        <  2:205043/149‑1 (MQ=255)
                                                                                                 cAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGaaa                                                                >  6:33656/1‑131 (MQ=255)
                                                                                                 cAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGaaa                                                                <  5:33656/131‑1 (MQ=255)
                                                                                                 cAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGc                                               >  6:30751/1‑148 (MQ=255)
                                                                                                 cAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCa                                              <  3:239959/149‑1 (MQ=255)
                                                                                                         gCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCAc                                      <  5:30751/149‑1 (MQ=255)
                                                                                                         gCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGACCCGATACGCACAACAACCGGGGTTGCACGCTGGTGACGCTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCAGGCTATCTCCAc                                      >  8:111888/1‑149 (MQ=255)
                                                                                                               aTTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGaa                                <  3:266901/149‑1 (MQ=255)
                                                                                                                   gTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACttt                            >  5:26229/1‑149 (MQ=255)
                                                                                                                     cgaCTGGCGCATCCCCCAGGGAGTGGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACTTTCg                          >  3:260621/3‑149 (MQ=255)
                                                                                                                       aCTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACTTTCggg                        >  6:293034/1‑149 (MQ=255)
                                                                                                                             gcCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTaa                                                         >  6:271813/1‑110 (MQ=255)
                                                                                                                             gcCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTaa                                                         <  5:271813/110‑1 (MQ=255)
                                                                                                                                             tGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACTTTCGGGTTCAAGGGCTACACCGATAGCg  <  1:9646/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AAGACCGCAACGATGCGCCGGTCACCGGCACGAACCGCGAACAGGTGCGCGACCTGCTGGATCGGGTGGATAAGGCTTTGTCCGACGACACGGATACCAAAATCGGCTGAGATTGGTATACTGGCGCCACTCCCTGGGGGATGCGCCAGTCGGTTATGTCCCTGAGCCGATACGCACAACCACGGGGGTTGCACGCTGGGGCCGGTGTGCATGTCCGCCCGACGGAAAGCCTTAACGCCCCCTGCAATCTCCACCTTGAACTTTCGGGTTCAAGGGCTACACCGATAGCG  >  NC_002947/5934548‑5934837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑