Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,938,098	T→C	9.4%	L220P (CTG→CCG)	rbbA →	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,938,098	0	T	C	9.4%	140.5 / 5.2	53	L220P (CTG→CCG)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base C (2/3);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GTTGACCCACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGGCCCGGTCGC  >  NC_002947/5937950‑5938242                                                                                                                                                     |                                                                                                                                                  gTTGACCCACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGgctgct                                                                                                                                                   >  8:261556/1‑149 (MQ=255)       ccACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCAGCAGCCGgccgc                                                                                                                                             >  3:265349/1‑149 (MQ=255)          cTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCgg                                                                                                                                          <  4:236979/149‑1 (MQ=255)             tCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCAGCAGCCGgccgccatc                                                                                                                                         <  4:265349/147‑4 (MQ=255)             tCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGACGGCGGCCAGCAGCCGgccgccatcga                                                                                                                                       <  1:212881/149‑6 (MQ=255)                 ggcggcgCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGc                                                                                                                                   <  2:16950/149‑1 (MQ=255)                       gcTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGaa                                                                                                                             >  6:77790/1‑149 (MQ=255)                              ggAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTgg                                                                                                                         <  7:218689/146‑1 (MQ=255)                                  cTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGt                                                                                                                  >  3:103555/1‑149 (MQ=255)                                    ggTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCa                                                                                                                >  1:245372/1‑149 (MQ=255)                                     tttGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACGGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCa                                                                                                                    <  7:155553/143‑1 (MQ=255)                                     gTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGc                                                                                                                        <  3:104867/140‑1 (MQ=255)                                     gTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGc                                                                                                                        >  4:104867/1‑140 (MQ=255)                                     gTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCa                                                                                                                    >  8:155553/1‑144 (MQ=255)                                     gTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCAc                                                                                                               >  5:283549/1‑149 (MQ=255)                                        gAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTc                                                                                                            >  8:123953/1‑149 (MQ=255)                                           cAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAg                                                                                                         <  4:103555/149‑1 (MQ=255)                                              gTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGgccgcc                                                                                                                                            <  7:118597/111‑1 (MQ=255)                                              gTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGgccgcc                                                                                                                                            >  8:118597/1‑111 (MQ=255)                                              gTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCgg                                                                                                      >  6:195471/1‑149 (MQ=255)                                              gTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCgg                                                                                                      >  6:157152/1‑149 (MQ=255)                                                   cgcACAACGCCCGCAACTGACCCTGCTGGTGGCTACCTCCTTCATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAAc                                                                                                 <  1:118793/149‑1 (MQ=255)                                                             ccGCAACTGACCCTGCTGGTGGCTACCTCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGc                                                                                       <  7:261556/149‑1 (MQ=255)                                                                     gACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTAcc                                                                                >  4:235318/1‑148 (MQ=255)                                                                      aCCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTAcc‑c                                                                              <  3:78290/148‑1 (MQ=255)                                                                        ccTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CAtt                                                                           <  2:70799/149‑1 (MQ=255)                                                                        ccTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CAtt                                                                           <  6:283549/149‑1 (MQ=255)                                                                            ctgGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCa                                                                       <  5:77790/149‑1 (MQ=255)                                                                               gtggCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAg                                                                      <  1:97459/147‑1 (MQ=255)                                                                                    tACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCgg                                                               >  5:129207/1‑149 (MQ=255)                                                                                      ccGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGc                                                             <  2:245372/149‑1 (MQ=255)                                                                                      ccGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGc                                                             <  3:128628/149‑1 (MQ=255)                                                                                             cATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGgcg                                                       <  5:195471/148‑1 (MQ=255)                                                                                              aTGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑Ca                                                                             >  8:163820/1‑125 (MQ=255)                                                                                              aTGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑Ca                                                                             <  7:163820/125‑1 (MQ=255)                                                                                                ggAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCgcag                                                    >  4:182290/1‑148 (MQ=255)                                                                                                    gaagCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CCTTACCAGGGCGCGGGCAAGGCGCAGCATCa                                               >  7:212464/1‑149 (MQ=255)                                                                                                    gaagCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATc                                                >  2:218648/1‑148 (MQ=255)                                                                                                          gAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCaa                                              <  7:57802/144‑1 (MQ=255)                                                                                                          gAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCaa                                              >  8:57802/1‑144 (MQ=255)                                                                                                          gAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACt                                         >  6:5221/1‑149 (MQ=255)                                                                                                          gAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACt                                         <  5:157152/149‑1 (MQ=255)                                                                                                           aaCAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTg                                        <  7:123953/149‑1 (MQ=255)                                                                                                                  cGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAGCCACTGGCAATcc                                 >  5:115564/1‑149 (MQ=255)                                                                                                                   gatcATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACCGCATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATc                                  <  1:218648/145‑1 (MQ=255)                                                                                                                   gAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATccc                                >  5:30453/1‑149 (MQ=255)                                                                                                                           ccTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCgcgc                         >  7:94265/1‑148 (MQ=255)                                                                                                                               aTGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCaa                      <  5:5221/147‑1 (MQ=255)                                                                                                                                      aTGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCacggac             >  2:153574/1‑149 (MQ=255)                                                                                                                                      aTGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCacggac             <  3:235318/149‑1 (MQ=255)                                                                                                                                           ggccggcTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGGccc        >  2:137688/1‑149 (MQ=255)                                                                                                                                           ggccggcTGCTGGCAGCCGGCCCTAGCCACAAACTGACTGCAGTCACCTCGAACGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCAACAACCACTGGCAAACCCGCCCCCCCAACCCACCGACCGccc        >  8:46658/1‑149 (MQ=255)                                                                                                                                           ggccggcGGCCAGCAGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCcgccgc                           >  6:311423/1‑130 (MQ=255)                                                                                                                                           ggccggcGGCCAGCAGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCcgccgc                           <  5:311423/130‑1 (MQ=255)                                                                                                                                                  tgctgGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGGCCCGGTCGc  >  7:218854/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GTTGACCCACTGTCGCGGCGGCGCTTCTGGGAGCTGGTTGAACAGGTTCGCGCACAACGCCCGCAACTGACCCTGCTGGTGGCTACCGCCTACATGGAAGAAGCCGAACAGTTCGAGCATTGCCTGATGCTCGATGGCGGCCGGCTGCTGGCCGCCGGCCCTAGCCACGAACTGGCTGCAGTCACCTCGAGCGGCAAACTGGATGATGCCTTTACC‑CATTACCAGGGCGCGGGCAAGGCGCAGCATCAACCACTGGCAATCCCGCCGCGCCAAGCCACGGACGGCCCGGTCGC  >  NC_002947/5937950‑5938242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑