Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,940,484	A→C	10.9%	D108A (GAC→GCC)	yhhJ →	translation‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,940,484	0	A	C	10.9%	162.6 / 8.3	61	D108A (GAC→GCC)	yhhJ	translation‑like protein
Reads supporting (aligned to +/- strand):  ref base A (25/29);  new base C (5/2);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCC  >  NC_002947/5940341‑5940627                                                                                                                                                |                                                                                                                                                ccTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACctgc                                                                                                                                            <  6:294035/149‑1 (MQ=255) ccTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACctgc                                                                                                                                            <  6:298280/149‑1 (MQ=255)      tctcGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGcc                                                                                                                                       >  7:211695/1‑149 (MQ=255)          gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCggac                                                                                                                                   <  5:302013/149‑3 (MQ=255)          gcgcAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCgg                                                                                                                                     <  3:26961/147‑1 (MQ=255)                gCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCGGCCTCCGCCGCGCCGGGACCCCGCCCTGGCCCGCGGCCCCTACACCGTCGGCCTCAAATTGCCGGGTTACTTCCACAGCGCCCTGCTGGCCGGgcgcgcgc                                                                                                                             >  6:40295/1‑149 (MQ=255)                 cTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGcc                                                                                                                            >  3:162645/1‑149 (MQ=255)                  tCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGAcc                                                                                                                                               >  8:269739/1‑129 (MQ=255)                  tCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGAcc                                                                                                                                               <  7:269739/129‑1 (MQ=255)                    aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTctgga                                                                                                                           >  4:152646/1‑143 (MQ=255)                    aGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTctgga                                                                                                                           <  3:152646/147‑5 (MQ=255)                      cGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGc                                                                                                                       <  8:50022/149‑1 (MQ=255)                       gAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCGGGACCACGCCATGGACAGCCGACAGTACACCTTCGTCATCAACGTGACGGTTAACTTGAAGAGCGCCCTGCTGGCCCGGCGCTAGCCGGac                                                                                                                        >  8:148807/1‑146 (MQ=255)                        aaGCCGTGCAAGCGGCCGAGTTCCAGCCAGCCGTGCCGGTGCCTCCGCAGCGCCTGGAGGAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGGCGGAGCTg                                                                                                                     <  4:162645/149‑1 (MQ=255)                         aGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAATGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGc                                                                                                                    <  5:40295/149‑1 (MQ=255)                             cTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGCGCTGCAGa                                                                                                                 >  5:168619/1‑148 (MQ=255)                             cTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGAt                                                                                                                <  1:255654/149‑1 (MQ=255)                                cAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCaa                                                                                                             <  3:66194/149‑1 (MQ=255)                                cAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCaa                                                                                                             <  3:70317/149‑1 (MQ=255)                                 aaCCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGc                                                                                                                             <  8:63915/132‑1 (MQ=255)                                 aaCCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGc                                                                                                                             >  7:63915/1‑132 (MQ=255)                                  aCCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACg                                                                                                           >  2:16038/1‑149 (MQ=255)                                       ccGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGAt                                                                                                      >  4:289456/1‑149 (MQ=255)                                               cagccagcCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTc                                                                                                                               >  8:208275/1‑116 (MQ=255)                                               cagccagcCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGCCCGGCCAGCAGGTCGCTc                                                                                                                               <  7:208275/116‑1 (MQ=255)                                                agccagccGTGCCGCTGCCTCCGCACCGCCTCGAACAGGCCAAGGACAGCGGCCATTACACCTTAGTCATAAACCTGCCGGTTAACTTCCAGAGCCACCTGCTGGCCGCGCGCTCGCACGAGCTGCAGAACAACGTCGAAGCATCGGc                                                                                              >  8:240337/1‑148 (MQ=255)                                                    agccGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGa                                                                                         <  3:130170/149‑1 (MQ=255)                                                    agccGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGa                                                                                         <  1:65357/149‑1 (MQ=255)                                                        gTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGcc                                                                                      >  2:289236/1‑148 (MQ=255)                                                         tGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCaa                                                                                    <  1:289236/149‑1 (MQ=255)                                                         tGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCaa                                                                                    <  2:192293/149‑1 (MQ=255)                                                                 gctacGCAGCGCCTGGACCAGGCCATGGACAGCGGACAGTACACGTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCGGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCa                                                                             <  7:240337/144‑1 (MQ=255)                                                                  cTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATg                                                                           <  8:211695/149‑1 (MQ=255)                                                                   tCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCGGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATgg                                                                          >  6:179054/1‑149 (MQ=255)                                                                     cGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGAAGCTACGGCCATGAGCCAAGCGTTCATGGgc                                                                        <  1:26500/149‑1 (MQ=255)                                                                           gcCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGc                                                                  >  5:83808/1‑149 (MQ=255)                                                                           gcCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGc                                                                  <  3:289456/149‑1 (MQ=255)                                                                            ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGc                                                                                       <  5:215852/127‑1 (MQ=255)                                                                            ccTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGc                                                                                       >  6:215852/1‑127 (MQ=255)                                                                                gACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACAt                                                             >  3:190696/1‑149 (MQ=255)                                                                                 aCCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATc                                                            >  4:191977/1‑149 (MQ=255)                                                                                     ggCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGcc                                                        <  1:150884/149‑1 (MQ=255)                                                                                      gCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCg                                                       >  1:235344/1‑149 (MQ=255)                                                                                       ccATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCTTGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCg                                                       >  5:98371/1‑148 (MQ=255)                                                                                          tGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATc                                                   >  5:195092/1‑149 (MQ=255)                                                                                             aCAGCGGCCGGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTc                                                <  5:179054/149‑1 (MQ=255)                                                                                             aCAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTc                                                <  6:195092/149‑1 (MQ=255)                                                                                                             cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                 >  8:289838/1‑148 (MQ=255)                                                                                                             cTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTc                                 >  3:236141/1‑148 (MQ=255)                                                                                                                 gTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTa                            <  6:83808/149‑1 (MQ=255)                                                                                                                  tcatcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGCGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGATCGACTa                            >  7:107194/1‑148 (MQ=255)                                                                                                                     tcaACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcagc                        >  8:212868/1‑149 (MQ=255)                                                                                                                       aaCGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTAcagc                        >  3:23555/1‑147 (MQ=255)                                                                                                                           tGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGt                  >  6:227620/1‑149 (MQ=255)                                                                                                                                   aaCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAAc            >  5:15112/1‑147 (MQ=255)                                                                                                                                   aaCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAAc            >  3:102064/1‑147 (MQ=255)                                                                                                                                    aCTTTCAGAGCGAGCTGCTGGCCGGGCGCTCGGCGGAGCTGCAGATGAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGa          <  6:309434/148‑1 (MQ=255)                                                                                                                                    aCTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCTTGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAg         <  6:98371/149‑1 (MQ=255)                                                                                                                                         gcgagCGCCCGGCCAGCAGGTCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAg    >  6:122446/3‑149 (MQ=255)                                                                                                                                         cAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCaaa     <  1:16038/148‑1 (MQ=255)                                                                                                                                           gagCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGcc  <  4:236141/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                CCTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGCCCGAGTTCCAGCCAGCCGTGCCGCTGCCTCCGCAGCGCCTGGACCAGGCCATGGACAGCGGCCAGTACACCTTCGTCATCAACGTGCCGGTTAACTTCCAGAGCGACCTGCTGGCCGGGCGCTCGCCGGAGCTGCAGATCAACGTCGATGCTACGGCCATGAGCCAAGCGTTCATGGGCGCCGGCTACATCGGCCGCATCTTCGAGCGCGAGCTGCTCGACTACAGCCAGCGTGGTAACGAGCAAAGCC  >  NC_002947/5940341‑5940627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑