Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,945,379 T→G 5.7% D55A (GAT→GCT) ‡ PP_5212 ← iron‑sulfur‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,945,3790TG 5.7% 223.2 / 3.5 70D55A (GAT→GCT) ‡PP_5212iron‑sulfur‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (35/31);  new base G (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGTCGACGCACTCACTGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGACGGCT  >  NC_002947/5945236‑5945526
                                                                                                                                               |                                                                                                                                                   
cGTCGACGCACTCACTGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTgg                                                                                                                                                <  7:98505/149‑1 (MQ=255)
       gCACTCACTGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGG‑GTTCGCCAGGATGGCGaagtcc                                                                                                                                        <  5:70249/149‑5 (MQ=255)
             actGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCAGCCCtt                                                                                                                                   <  5:15513/149‑1 (MQ=255)
             actGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCAGCCCtt                                                                                                                                   >  6:15513/1‑149 (MQ=255)
              ctGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACACCTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTc                                                                                                                                  <  5:6456/149‑1 (MQ=255)
              ctGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGATCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTc                                                                                                                                  <  5:2148/149‑1 (MQ=255)
                 aCGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTcaac                                                                                                                               >  1:47935/1‑149 (MQ=255)
                     tGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTgg                                                                                                                                                >  8:61212/1‑128 (MQ=255)
                     tGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTgg                                                                                                                                                <  7:61212/128‑1 (MQ=255)
                     tGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTcaaccaa                                                                                                                            >  4:189297/1‑148 (MQ=255)
                        aCGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCaacaac                                                                                                                        <  7:149792/149‑1 (MQ=255)
                         cGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCAGGATGGCGaagt                                                                                                                                          <  8:237049/130‑3 (MQ=255)
                         cGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCAGGATGGCGaagt                                                                                                                                          >  7:237049/1‑128 (MQ=255)
                            cAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACgcgg                                                                                                                    >  6:300365/1‑149 (MQ=255)
                             aGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACgcggc                                                                                                                   >  8:64395/1‑149 (MQ=255)
                                ttGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGACCCCGCCCTTAAACCAACAACGCGgcgc                                                                                                                 >  7:192285/1‑148 (MQ=255)
                                ttGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGgcgc                                                                                                                 >  2:277003/1‑148 (MQ=255)
                                 tGCTCACTGGCAGTTCTCCCAAGGCAAGAACAACTTCCCAGAGCAATAGTAATTCCTCCAGAGGTTGTGAAATGAATTTTAACAACTCGCCATGGTCGCGTAGTTCTCCATCATGTAGAACCAGTAGTTAAACCAACAAagcttcgta                                                                                                                <  4:107944/148‑7 (MQ=255)
                                    gcACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAAcgcg                                                                                                                     <  1:189643/140‑1 (MQ=255)
                                    gcACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAAcgcg                                                                                                                     >  2:189643/1‑140 (MQ=255)
                                       cTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTAGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATgg                                                                                                         <  8:51360/149‑1 (MQ=255)
                                            aGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAAtt                                                                                                    <  2:281311/149‑1 (MQ=255)
                                            aGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAAtt                                                                                                    >  1:56663/1‑149 (MQ=255)
                                                cGCCCAAGGCAAGCACACCGTCCCAGAGCAACGCGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGc                                                                                                  <  3:7827/147‑1 (MQ=255)
                                                cGCCCAAGGCAAGCACACAGTCCCAGAGCAACGCGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGCCAATTGc                                                                                                  >  4:7827/1‑147 (MQ=255)
                                                   ccAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTg                                                                                             >  8:147481/1‑149 (MQ=255)
                                                    cAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGc                                                                                            >  4:298948/1‑149 (MQ=255)
                                                    cAAGGCAAGCACACCGGCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGc                                                                                            <  5:129810/149‑1 (MQ=255)
                                                         cAAGCACACCGTCCCAGAGCAACACGCAGGCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAg                                                                                       <  7:64395/149‑1 (MQ=255)
                                                          aaGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGc                                                                                      >  3:190324/1‑149 (MQ=255)
                                                             cacaCCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGt                                                                                   <  7:147481/149‑1 (MQ=255)
                                                                acCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTg                                                                                >  7:29980/1‑149 (MQ=255)
                                                                acCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTg                                                                                >  8:113093/1‑149 (MQ=255)
                                                                 ccGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTgg                                                                               <  3:189297/149‑1 (MQ=255)
                                                                 ccGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTgg                                                                               >  3:128003/1‑149 (MQ=255)
                                                                  cGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTAGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTggg                                                                              <  4:128003/149‑1 (MQ=255)
                                                                    tCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCa                                                                            <  7:113093/149‑1 (MQ=255)
                                                                          agCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATa                                                                      <  5:300365/149‑1 (MQ=255)
                                                                          agCAACACGCAGTCCTCCAGCGGTTAGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTTGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGATGTTTGGGCAGTCa                                                                        >  8:114118/1‑147 (MQ=255)
                                                                           gCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAg                                                                     <  6:309871/149‑1 (MQ=255)
                                                                                  gCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAgcc                                                              >  7:106400/1‑149 (MQ=255)
                                                                                         tccAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAgccg                                                             <  6:111290/143‑1 (MQ=255)
                                                                                         tccAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAgccg                                                             >  5:111290/1‑143 (MQ=255)
                                                                                         tccAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGc                                                       >  5:312423/1‑149 (MQ=255)
                                                                                         tccAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGc                                                       >  4:110684/1‑149 (MQ=255)
                                                                                           cAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGc                                                       <  8:106400/147‑1 (MQ=255)
                                                                                               ggTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCtccatc                                                 <  6:312423/149‑1 (MQ=255)
                                                                                                gTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCtccatcc                                                >  7:243872/1‑149 (MQ=255)
                                                                                                         tGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCtt                                       <  1:277003/149‑1 (MQ=255)
                                                                                                           cAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCttt                                      <  3:298948/148‑1 (MQ=255)
                                                                                                            aGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGc                                                       >  8:67444/1‑130 (MQ=255)
                                                                                                            aGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGc                                                       <  7:67444/130‑1 (MQ=255)
                                                                                                              gTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCtccatc                                                 >  3:189752/1‑134 (MQ=255)
                                                                                                              gTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCtccatc                                                 <  4:189752/134‑1 (MQ=255)
                                                                                                                 aacaacTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCggg                               >  4:124440/1‑149 (MQ=255)
                                                                                                                 aacaacTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCggg                               >  8:84369/1‑149 (MQ=255)
                                                                                                                      cTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATATCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTGCa                           >  6:216847/1‑148 (MQ=255)
                                                                                                                       tCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAg                         >  7:22867/1‑149 (MQ=255)
                                                                                                                         gCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAgcg                       >  3:170779/1‑149 (MQ=255)
                                                                                                                         gCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAgc                        <  8:29980/148‑1 (MQ=255)
                                                                                                                           cATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAgcgc                      >  7:200991/1‑148 (MQ=255)
                                                                                                                           cATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAgcgc                      >  7:197452/1‑148 (MQ=255)
                                                                                                                           cATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGcc                     >  3:271095/1‑149 (MQ=255)
                                                                                                                               gTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCa                 <  3:110684/149‑1 (MQ=255)
                                                                                                                                     gACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGAGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCACCGGGTTCAAGCGACAGCACCGccc           >  8:175287/1‑149 (MQ=255)
                                                                                                                                       gttCGCCAGGATGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCg         >  1:15176/2‑149 (MQ=255)
                                                                                                                                          cGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGa       <  8:22867/148‑1 (MQ=255)
                                                                                                                                           gCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGACg     >  4:77868/1‑149 (MQ=255)
                                                                                                                                            ccATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGACgg    <  7:116284/149‑1 (MQ=255)
                                                                                                                                              aTCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGTATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGACGGCt  <  4:190324/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CGTCGACGCACTCACTGACGCTGCACGCCAGCTTGCGCACTGGCAGTTCGCCCAAGGCAAGCACACCGTCCCAGAGCAACACGCAGTCCTCCAGCGGTTCGGCAATGCAGGTGAACAACTCGCCATGGTCGCGGACTTCGCCATCCTGGCGAACCCGCCCTTCAACCAACAACGCGGCGCAAACATGGCAATTGCCATTGCGGCAGCTGTTTGGGCAGTCATAGCCCAGCCGCCGTGCTCCATCCAGGATCCTTTCCCCGGGTTCAAGCGCCAGCACCGCCCCGGACGGCT  >  NC_002947/5945236‑5945526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑