Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,963,851 C→A 12.3% I217I (ATC→ATA)  PP_5229 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,963,8510CA12.3% 156.2 / 11.1 57I217I (ATC→ATA) PP_5229hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base A (4/3);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

TCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGA  >  NC_002947/5963703‑5963996
                                                                                                                                                    |                                                                                                                                                 
tacTGGTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATc                                                                                                                                                   <  7:230462/147‑1 (MQ=255)
  cTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCg                                                                                                                                                  <  6:222562/148‑1 (MQ=255)
     ttCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGa                                                                                                                                              <  5:36429/149‑1 (MQ=255)
       cGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAg                                                                                                                                             <  4:273776/148‑1 (MQ=255)
       cGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAg                                                                                                                                             <  2:131884/148‑1 (MQ=255)
       cGGTGAAGAACAGCGCCGGGAAGTAGGCGCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAg                                                                                                                                             <  5:117209/148‑1 (MQ=255)
         gTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTg                                                                                                                                          >  2:186928/1‑149 (MQ=255)
          tGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGc                                                                                                                                         >  7:258950/1‑149 (MQ=255)
              gaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTgg                                                                                                                                     <  1:220191/149‑1 (MQ=255)
                aCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGcc                                                                                                                                   <  7:273901/149‑1 (MQ=255)
                     gcCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGtt                                                                                                                              <  6:282754/149‑1 (MQ=255)
                     gcCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGtt                                                                                                                              <  1:186928/149‑1 (MQ=255)
                              tAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAcgc                                                                                                                     <  6:294295/149‑1 (MQ=255)
                                ggCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCg                                                                                                                                               >  3:172456/1‑121 (MQ=255)
                                ggCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCg                                                                                                                                               <  4:172456/121‑1 (MQ=255)
                                        cgcCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGa                                                                                                           <  5:113211/149‑1 (MQ=255)
                                             tGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTg                                                                                                      >  6:65931/1‑149 (MQ=255)
                                             tGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTg                                                                                                      >  3:257174/1‑149 (MQ=255)
                                                        gAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACg                                                                                           >  5:272502/1‑149 (MQ=255)
                                                         aGTACCAGGGGCTGTATGGTGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCATCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACg                                                                                           <  4:434/148‑1 (MQ=255)
                                                           tACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCt                                                                                         <  4:257174/148‑1 (MQ=255)
                                                            aCCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtga                                                                                       >  8:207683/1‑149 (MQ=255)
                                                                     tGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGc                                                                               >  6:197285/1‑148 (MQ=255)
                                                                          ggggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGt                                                                                                                   >  2:225296/1‑107 (MQ=255)
                                                                          ggggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGt                                                                                                                   <  1:225296/107‑1 (MQ=255)
                                                                                cTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCt                                                                   >  6:249942/1‑149 (MQ=255)
                                                                                 tGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCt                                                                   >  5:235940/1‑148 (MQ=255)
                                                                                    ccATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAtt                                                               <  6:272502/149‑1 (MQ=255)
                                                                                    ccATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTAtt                                                               >  5:233658/1‑149 (MQ=255)
                                                                                         ggCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAggaa                                                                                                                                          >  6:42520/1‑67 (MQ=38)
                                                                                         ggCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAggaa                                                                                                                                          <  5:42520/69‑3 (MQ=38)
                                                                                              ccGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCt                                                     <  5:65931/149‑1 (MQ=255)
                                                                                                     ccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAcc                                                                                                               >  3:250674/1‑84 (MQ=255)
                                                                                                     ccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAcc                                                                                                               <  4:250674/84‑1 (MQ=255)
                                                                                                     ccGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                              <  7:207683/149‑1 (MQ=255)
                                                                                                     ccGCAACACTACAAGAGCAGCGGCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTCCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                              <  5:125715/149‑1 (MQ=255)
                                                                                                      cGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcg                                                            >  3:190824/1‑134 (MQ=255)
                                                                                                      cGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcg                                                            <  4:190824/134‑1 (MQ=255)
                                                                                                       gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAg                                                                            >  4:244452/1‑117 (MQ=255)
                                                                                                       gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAg                                                                            <  3:244452/117‑1 (MQ=255)
                                                                                                       gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                              >  6:217293/1‑147 (MQ=255)
                                                                                                             cTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAgcgc                                        >  2:260965/1‑147 (MQ=255)
                                                                                                              tACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtga                                                                                       >  1:122577/1‑99 (MQ=255)
                                                                                                                   gagCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGa                                            <  7:207484/137‑1 (MQ=255)
                                                                                                                   gagCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGa                                            >  8:207484/1‑137 (MQ=255)
                                                                                                                    agcagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGCGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCt                                                     <  5:283902/127‑1 (MQ=255)
                                                                                                                    agcagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGCGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCt                                                     >  6:283902/1‑127 (MQ=255)
                                                                                                                     gcagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCgg                              <  5:197285/149‑1 (MQ=255)
                                                                                                                                 cccTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGc                  <  1:260965/149‑1 (MQ=255)
                                                                                                                                   cTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGGACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAg                <  5:217293/149‑1 (MQ=255)
                                                                                                                                       accTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGa                             <  2:292257/131‑1 (MQ=255)
                                                                                                                                       accTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGa                             >  1:292257/2‑132 (MQ=255)
                                                                                                                                       accTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGc             >  5:179138/2‑148 (MQ=255)
                                                                                                                                        ccTCGGCTATATAGCGGTGGTGCTTGGCCGCGTTGTTCCACGCGTCACCGAGACCCTTCGCCCGGTACTCTGATGAAACGCCAGCTGGAGGGTTATTGCGCACACCTGCGCAACGAGTGCCAGGTGTCGGAGCACACGTCTCAGGGCt            <  8:181701/148‑1 (MQ=255)
                                                                                                                                        ccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGACACGCGTCACCCAGACCCTGCGCCCAGTACGCTGATGAAACGCAAGCTGGAGGATTAATGCGCACACCTGCGCAACGAGCGCAAGGTGTAGGAGCACACCTCGCAGGGCTa           >  7:181701/1‑149 (MQ=255)
                                                                                                                                          tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc         >  1:180385/1‑149 (MQ=255)
                                                                                                                                             gCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTc      >  1:156040/1‑149 (MQ=255)
                                                                                                                                                 tatCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGa  >  6:166572/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGA  >  NC_002947/5963703‑5963996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑