Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,979,378 A→G 6.5% H8R (CAC→CGC)  PP_5242 → GAF domain‑containing sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,979,3780AG 6.5% 173.6 / 2.1 61H8R (CAC→CGC) PP_5242GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GCCTGCCCCAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTGCGCGCGCCGC  >  NC_002947/5979230‑5979524
                                                                                                                                                    |                                                                                                                                                  
gCCTGCCCCAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCca                                                                                                                                                    >  3:272343/1‑149 (MQ=255)
      cccAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGcc                                                                                                                                              >  1:173682/1‑149 (MQ=255)
       ccAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGc                                                                                                                                               <  6:203650/147‑1 (MQ=255)
       ccAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGc                                                                                                                                               >  5:203650/1‑147 (MQ=255)
         aaGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCAt                                                                                                                                           >  6:118626/1‑149 (MQ=255)
          aGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATc                                                                                                                                          <  4:227331/149‑1 (MQ=255)
            gggACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGa                                                                                                                                        >  6:311690/1‑149 (MQ=255)
             ggACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGAt                                                                                                                                       <  5:275627/149‑1 (MQ=255)
                cGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTAc                                                                                                                                    <  2:140962/149‑1 (MQ=255)
                           aacaGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAAt                                                                                                                         <  2:173682/149‑1 (MQ=255)
                           aacaGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAAt                                                                                                                         >  2:245338/1‑149 (MQ=255)
                            acaGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATc                                                                                                                        <  5:311690/149‑1 (MQ=255)
                               gCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTATGACCAGACAATcgac                                                                                                                     <  2:38127/149‑1 (MQ=255)
                                       gTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGggctg                                                                                                             >  7:78380/1‑149 (MQ=255)
                                               cAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGc                                                                                                     <  4:215272/149‑1 (MQ=255)
                                                   cAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTg                                                                                                 <  2:158452/149‑1 (MQ=255)
                                                       cTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAg                                                                                             <  8:78380/149‑1 (MQ=255)
                                                        tGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAgg                                                                                            >  8:58109/1‑149 (MQ=255)
                                                          tAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGCCGAGGGCTGGCTGaa                                                                                                       <  3:59797/136‑1 (MQ=255)
                                                          tAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGaa                                                                                                       >  4:59797/1‑136 (MQ=255)
                                                              ccGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAgcg                                                                                      <  8:122117/149‑1 (MQ=255)
                                                                      aTGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGc                                                                              >  5:223334/1‑149 (MQ=255)
                                                                           gggCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAgg                                                                          >  8:97588/1‑148 (MQ=255)
                                                                            ggCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAgg                                                                          <  4:272343/147‑1 (MQ=255)
                                                                               ccGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGt                                                                     <  7:58109/149‑1 (MQ=255)
                                                                                       tataCTCGCGCCTCAGGATGTCGCTAGTGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAg                                                             <  8:8311/149‑1 (MQ=255)
                                                                                           cTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTa                                                          <  7:97588/148‑1 (MQ=255)
                                                                                            tCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAtt                                                        >  2:300510/1‑149 (MQ=255)
                                                                                            tCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAtt                                                        >  4:191455/1‑149 (MQ=255)
                                                                                            tCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAtt                                                        >  3:38394/1‑149 (MQ=255)
                                                                                            tCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAtt                                                        >  2:164027/1‑149 (MQ=255)
                                                                                            tCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAtt                                                        >  5:120737/1‑149 (MQ=255)
                                                                                             cgcgCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGATGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTAttt                                                       >  3:45386/1‑149 (MQ=255)
                                                                                               cgcCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTcc                                                     <  6:120737/149‑1 (MQ=255)
                                                                                               cgcCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTcc                                                     <  6:108138/149‑1 (MQ=255)
                                                                                                  cTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGcc                                                  >  1:52006/1‑149 (MQ=255)
                                                                                                  cTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGcc                                                  <  3:191455/149‑1 (MQ=255)
                                                                                                         tGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGa                                           <  6:223334/149‑1 (MQ=255)
                                                                                                             gCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGAcc                                         >  5:160042/1‑147 (MQ=255)
                                                                                                                 gCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCACTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGc                                     <  1:164027/147‑1 (MQ=255)
                                                                                                                   gggggTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCcac                                  >  5:52946/1‑148 (MQ=255)
                                                                                                                      ggTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAAt                              <  5:118626/149‑1 (MQ=255)
                                                                                                                         gCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAg                           <  7:274685/149‑1 (MQ=255)
                                                                                                                          cTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTg                                                <  1:193948/127‑1 (MQ=255)
                                                                                                                          cTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTg                                                >  2:193948/1‑127 (MQ=255)
                                                                                                                           tttATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGc                         >  3:198345/1‑149 (MQ=255)
                                                                                                                              aTGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCt                       <  2:52006/148‑1 (MQ=255)
                                                                                                                                  tCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTg                                                                    <  1:35126/99‑1 (MQ=255)
                                                                                                                                  tCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTg                                                                    >  2:35126/1‑99 (MQ=255)
                                                                                                                                  tCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATc                  >  8:30463/1‑149 (MQ=255)
                                                                                                                                         aCGCCTTGCCCGCAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCt                       >  3:222574/1‑137 (MQ=255)
                                                                                                                                         aCGCCTTGCCCGCAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCt                       >  3:226697/1‑137 (MQ=255)
                                                                                                                                         aCGCCTTGCCCGCAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCt                       <  4:226697/137‑1 (MQ=255)
                                                                                                                                         aCGCCTTGCCCGCAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCt                       <  4:222574/137‑1 (MQ=255)
                                                                                                                                         aCGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTg            <  4:38394/148‑1 (MQ=255)
                                                                                                                                          cGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTgcg          >  6:67092/1‑149 (MQ=255)
                                                                                                                                             cTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCCGCCACAAATCAGGCCTGGATCGCCGTgcgcgc       >  8:277123/1‑149 (MQ=255)
                                                                                                                                             cTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTgcgcgc       >  5:124916/1‑149 (MQ=255)
                                                                                                                                              ttGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCATGGATCGCCGTgcgcgcg      >  7:142091/1‑149 (MQ=255)
                                                                                                                                                  ccACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTGCGCGcgccgc  >  7:225836/1‑149 (MQ=255)
                                                                                                                                                  ccACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTGCGCGcgcc    >  6:271009/1‑147 (MQ=255)
                                                                                                                                                  ccACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTGCGCGcgcc    <  5:271009/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCCTGCCCCAAGGGGACGATAACGGACAACAGCGTGCCCGTTCCTGCCAAGCAGGCTGTAACCCGCATCCATGCTGGGCCCGCTGCGTATACTCGCGCCTCAGGATGTCGCTAGCGGGGGTGCTTTATGCTCGATGAACGCCTTGCCCACAGGCCCATCGATTACGACCAGTCAATCGACGAGGGCTGGCTGAAGCGGTGCGAGGTCAGCGCGCTGGGCCAGGAAGGTGAACTCAGCTATTTCCGCCTGCGCGACCCAGCCACAAATCAGGCCTGGATCGCCGTGCGCGCGCCGC  >  NC_002947/5979230‑5979524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑