Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,984,455 G→A 9.1% intergenic (+51/+38) PP_5242 → / ← ycaC‑II GAF domain‑containing sensor histidine kinase/putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,984,4550GA 9.1% 120.9 / 2.8 44intergenic (+51/+38)PP_5242/ycaC‑IIGAF domain‑containing sensor histidine kinase/putative hydrolase
Reads supporting (aligned to +/- strand):  ref base G (22/18);  new base A (2/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GTTCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTG  >  NC_002947/5984307‑5984600
                                                                                                                                                    |                                                                                                                                                  
gTTCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTTCGGTCGGCTGTAggg                                                                                                                                                    >  7:272116/1‑149 (MQ=255)
        gCTCAGGCCCACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCggg                                                                                                                                               >  7:277280/1‑146 (MQ=38)
        gCTCAGGCCCACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCCCCTACAGCCGACCGTAGGAGCggg                                                                                                                                               <  8:277280/146‑1 (MQ=38)
                ccACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTAGCGGGTAAAACCCCAGCGAACGTCGGCGGGAGGGGCGGGGTTTCCcccg                                                                                                                                     >  4:125324/1‑148 (MQ=255)
                        ggCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCGGCCTTTGAATCACGCATCGCCTGTGCCGGCCTCTCCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCa                                                                                                                            <  5:123995/149‑1 (MQ=255)
                              tGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGGTGCTACGGTCGGCTGTAGGGGCGGGTTTACCCGGGAAGAGGCCCGTTC‑Ag                                                                                                                     >  8:225889/1‑149 (MQ=38)
                                   gcgcTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTAGCGGGTAAAACCGCTCCAAACGTAGGCGGTATGGGCGGGGTTAACCGCGAAGAGGGCAGTAC‑ACGttt                                                                                                                 >  1:127889/1‑148 (MQ=255)
                                                         gATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTg                                                                                          >  1:175014/1‑149 (MQ=255)
                                                             ccGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTGCTTCGGTCGGCTTTAGGGGGGGGTTTAGCCGCGAAGAGGCGAGTAC‑AGGTTTACATCAGCTCGAGGCCAGCGCTGCGTa                                                                                      <  6:235318/149‑1 (MQ=38)
                                                             ccGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGt                                                                                       <  2:205045/148‑1 (MQ=255)
                                                             ccGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGt                                                                                       >  1:205045/1‑148 (MQ=255)
                                                             ccGGATTCGTGTCAGGCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCg                                                                                        <  6:115707/147‑1 (MQ=255)
                                                                     gtggCAGTCTGCCATTGAATCAGGCATAGCCTGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGGTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTtc                                                                               <  8:287058/148‑1 (MQ=38)
                                                                      tgtCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTtcat                                                                             >  2:19187/1‑149 (MQ=255)
                                                                        tCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCTCTGCGTAGCTGTtcat                                                                             >  7:40531/1‑147 (MQ=255)
                                                                        tCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCTCTGCGTAGCTGTtcat                                                                             >  7:35722/1‑147 (MQ=255)
                                                                            tCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCCGTAC‑AGGTTTACATCAGCCCGCGGCCCGCGCTGCGTAGCAGTTCATCACGtt                                                                       >  5:270000/1‑149 (MQ=37)
                                                                            tCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTTAATAATCGCGAGGCCAGCGCTGCGTAGCTGTTCATCATGtt                                                                       >  5:265220/1‑149 (MQ=37)
                                                                            tCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCGCCTAAGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCCGCGCTGGGTAGCTGTTCATGAGGtt                                                                       >  5:164804/1‑149 (MQ=38)
                                                                              tGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCGGTAGGGGCGGGGTTACCCGCGCAGAGGCCAGTAC‑AGGTTTTCAACAGCCCGAGGCCAGCGCTGCGTAGCTGTTCCTAATGTTGc                                                                     >  8:145500/1‑149 (MQ=37)
                                                                               gCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAgc                                                                                              <  7:242906/123‑1 (MQ=255)
                                                                               gCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAAGGGCGGGTTTAACCGCCAAGACGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAgc                                                                                              >  8:242906/1‑123 (MQ=255)
                                                                                ccTTTGAATCACGCATAGCCTGTGCCGGCCTGTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCgg                                                                   <  2:191882/149‑1 (MQ=255)
                                                                                ccTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGTCAGCGCTGCGTAGCTGTTCATCAGGTTGCgg                                                                   >  7:278012/1‑149 (MQ=255)
                                                                                  tttGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCg                                                                    >  5:157970/1‑146 (MQ=255)
                                                                                  tttGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCg                                                                    <  6:157970/146‑1 (MQ=255)
                                                                                    tGAATCACGCATAGCCTGGGCCGGCCTCTTCGCGGTTAACCCCGCTCCTACGTGCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTa                                                                 <  3:125324/147‑1 (MQ=38)
                                                                                     gAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGt                                                               <  8:272116/148‑1 (MQ=255)
                                                                                                  ccTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTCGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCTCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGc                                                 <  8:40531/149‑1 (MQ=255)
                                                                                                  ccTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCGGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCTCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGc                                                 <  8:35722/149‑1 (MQ=255)
                                                                                                         cGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGGAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcag                                            >  6:280877/1‑147 (MQ=255)
                                                                                                         cGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCGGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcagca                                          <  2:175014/149‑1 (MQ=255)
                                                                                                          ggCCTCTTCGCGGGTAATCGCGGTCCTAGGGTCGGGTGTTGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcagcag                                         <  6:270000/149‑1 (MQ=255)
                                                                                                          ggCCTATTCGCTGTTAAACCCGATCATAGGGTCGGGTGTAGTGGCGGGTTTACCCGCGAAGATGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCTCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGAcagcag                                         <  6:265220/149‑1 (MQ=255)
                                                                                                           gCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAgg                                        >  6:121475/1‑149 (MQ=255)
                                                                                                                 tCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTAcc                                   >  5:289240/1‑148 (MQ=255)
                                                                                                                        tAACCCCGCTCCTCCGGGCGGCTGTCGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCtt                           <  7:145500/149‑1 (MQ=255)
                                                                                                                          aaCCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCg                         <  7:29833/149‑1 (MQ=255)
                                                                                                                            cccGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGAt                       >  1:135205/1‑149 (MQ=255)
                                                                                                                             ccGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATATGCTGCGACAGCAGGTTACCCAGCCCTTCGATg                      >  2:233150/1‑149 (MQ=255)
                                                                                                                             ccGCCCCTACAGCCGACCGTAGGAGCGGGATTACCCGCGAAGAGGCCGGCACTGGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGAt                       >  1:234509/1‑149 (MQ=25)
                                                                                                                                   cTAGGGTCGGGTGTTGGGGCGGTTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTg                <  6:164804/149‑1 (MQ=255)
                                                                                                                                              tGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTcgcg     >  8:165400/1‑149 (MQ=255)
                                                                                                                                               gTAGGAGCGGGATTACCCGCGAAGAGGCCGGCAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCgg    <  2:234509/149‑1 (MQ=255)
                                                                                                                                                 aGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTg  >  2:273741/1‑149 (MQ=255)
                                                                                                                                                   gggCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATGAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTGCGATGTCGTTGCGCCAGTAGCGGTg  >  7:251470/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTCGGTGGCTCAGGCCCACGGCGGGCAGCTGCAGGCGCTGGTGTCGCAGTTGGGTGGATGCCGGATTCGTGTCAGTCTGCCTTTGAATCACGCATAGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACGGTCGGCTGTAGGGGCGGGTTTACCCGCGAAGAGGCCAGTAC‑AGGTTTACATCAGCGCGAGGCCAGCGCTGCGTAGCTGTTCATCAGGTTGCGGTAGTTGGGGATACGCTGCGACAGCAGGTTACCCAGCCCTTCGATGTCGTTGCGCCAGTCGCGGTG  >  NC_002947/5984307‑5984600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑