Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,985,761 G→A 8.9% V10M (GTG→ATG)  PP_5245 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,985,7610GA 8.9% 157.4 / 5.0 56V10M (GTG→ATG) PP_5245AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (25/26);  new base A (2/3);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CAAACCTGAATTGTTGTTAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGC  >  NC_002947/5985613‑5985901
                                                                                                                                                    |                                                                                                                                            
cAAACCTGAATTGTTGTTAATTGTGCGCTGCTGGACCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCcaccgcaccg                                                                                                                                              >  2:49256/1‑149 (MQ=255)
    ccTGAATTGTTGTTAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGc                                                                                                                                          <  1:224254/149‑1 (MQ=255)
        aaTTGTTGTTAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATg                                                                                                                                      <  1:49256/149‑1 (MQ=255)
          ttgttgTTAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGt                                                                                                                                    >  4:27595/1‑149 (MQ=255)
             ttgttAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCATGGCCACGGTGc                                                                                                                                  <  3:290352/148‑1 (MQ=255)
                       tgCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGAt                                                                                                                       <  8:275322/149‑1 (MQ=255)
                         cgcTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCATGGc                                                                                                                                          >  2:107885/1‑128 (MQ=255)
                         cgcTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCATGGc                                                                                                                                          <  1:107885/128‑1 (MQ=255)
                               tgGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTAct                                                                                                               >  6:152882/1‑149 (MQ=255)
                                 gCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTActgc                                                                                                             >  2:208423/1‑149 (MQ=255)
                                  cccTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTActgct                                                                                                            <  6:4946/149‑1 (MQ=255)
                                   ccTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTActgctg                                                                                                           >  3:16051/1‑149 (MQ=255)
                                       ggCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGa                                                                                                         <  4:265580/147‑1 (MQ=255)
                                       ggCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGAt                                                                                                        <  4:4453/148‑1 (MQ=255)
                                       ggCGGTGTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGAt                                                                                                        <  7:269822/148‑1 (MQ=255)
                                           ttttGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCAc                                                                                                   >  7:231640/1‑149 (MQ=255)
                                                ccGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCAt                                                                                                                                       >  6:37175/1‑108 (MQ=255)
                                                ccGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCAt                                                                                                                                       <  5:37175/108‑1 (MQ=255)
                                                  gATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAAc                                                                                                                          <  1:191326/119‑1 (MQ=255)
                                                  gATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAAc                                                                                                                          >  2:191326/1‑119 (MQ=255)
                                                  gATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTccc                                                                                            <  8:231640/149‑1 (MQ=255)
                                                    tACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCAt                                                                                          <  7:256517/149‑1 (MQ=255)
                                                        ttAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGAt                                                                                      >  5:294367/1‑149 (MQ=255)
                                                          aaGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGAt                                                                                      <  3:27595/147‑1 (MQ=255)
                                                               gCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATtcg                                                                               >  4:27106/1‑149 (MQ=255)
                                                                 gTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATtcg                                                                               >  6:49159/1‑147 (MQ=255)
                                                                 gTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATtcg                                                                               <  5:49159/147‑1 (MQ=255)
                                                                         ttCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCAAGGATGCATTCGCCATCGCCaa                                                                     >  5:313639/1‑149 (MQ=255)
                                                                            aGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGATAGAGGTATGCAGGAAAAACCCGCCCACCGCACCATGGCCACGGTGCGGt                                                                                                                               <  5:133646/88‑1 (MQ=255)
                                                                            aGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAAcc                                                                   <  6:251742/148‑1 (MQ=255)
                                                                            aGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAACAGGTATGCAGGAAAAACCCGCCCACCGCACCATCGCCACCGTGCGGt                                                                                                                               >  6:133646/1‑88 (MQ=255)
                                                                             gCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCgg                                                                 <  5:152882/149‑1 (MQ=255)
                                                                              cTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGt                                                                <  3:292115/149‑1 (MQ=255)
                                                                                 cTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCt                                                             >  3:131484/1‑149 (MQ=255)
                                                                                   ggCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTg                                                           >  7:207312/1‑149 (MQ=255)
                                                                                    gCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGc                                                          >  4:193297/1‑149 (MQ=255)
                                                                                     ccGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGcc                                                         >  4:224657/1‑149 (MQ=255)
                                                                                       gCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGCGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTg                                                       <  3:27106/149‑1 (MQ=255)
                                                                                            ccGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTgcagca                                                  >  5:157838/1‑149 (MQ=255)
                                                                                             cgcgGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCAc                                                 >  6:171432/1‑149 (MQ=255)
                                                                                              gcgGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACg                                                >  2:45115/1‑149 (MQ=255)
                                                                                               cgGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACgg                                               >  7:109591/1‑149 (MQ=255)
                                                                                                 gTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCa                                             <  1:208423/149‑1 (MQ=255)
                                                                                                      cATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATc                                        >  7:206552/1‑149 (MQ=255)
                                                                                                      cATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATc                                        <  3:193297/149‑1 (MQ=255)
                                                                                                         tCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACg                                                <  3:134844/138‑1 (MQ=255)
                                                                                                         tCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACg                                                >  4:134844/1‑138 (MQ=255)
                                                                                                            aGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGgc                                     >  3:247316/1‑146 (MQ=255)
                                                                                                            aGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGgc                                     <  4:247316/146‑1 (MQ=255)
                                                                                                                         aTGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATtcgccatc                                                                          <  6:107844/96‑1 (MQ=255)
                                                                                                                         aTGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATtcgccatc                                                                          >  5:107844/1‑96 (MQ=255)
                                                                                                                            cAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAcgg                  >  4:56840/1‑149 (MQ=255)
                                                                                                                                    aCCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAg          <  4:131484/149‑1 (MQ=255)
                                                                                                                                    aCCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAg          >  2:213049/1‑149 (MQ=255)
                                                                                                                                      ccGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTg        <  4:16051/149‑1 (MQ=255)
                                                                                                                                       cGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTgg       <  1:213049/149‑1 (MQ=255)
                                                                                                                                       cGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTgg       <  3:224657/149‑1 (MQ=255)
                                                                                                                                            accgcaccgTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTAcgc  >  2:296228/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
CAAACCTGAATTGTTGTTAATTGTGCGCTGCTGGCCCTGGGCGTTTTGCCGATACCTTAAGATGCGTTTCGTATTCAGCTTCTGGCCGCTACCCGCGGTCGGCATTCAAGGCGAAAGAGGTATGCAGGAAAAACCCGCCCACCGCACCGTGGCCATGGTGCTGTTCAACGATGTGTTACTGCTGGATGTCACCGGTCCCATGGATGCATTCGCCATCGCCAACCGGTTCTTGCCTGCAGCACGGCAATATCGGCTGCTGACCTTGGCCGAGGGGCAAGCAGTGGTACGC  >  NC_002947/5985613‑5985901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑