Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 460,892 T→C 7.1% S525G (AGC→GGC)  pqqF ← coenzyme PQQ synthesis protein F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947460,8920TC 7.1% 157.0 / 2.4 56S525G (AGC→GGC) pqqFcoenzyme PQQ synthesis protein F
Reads supporting (aligned to +/- strand):  ref base T (28/24);  new base C (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

CCCAGGGCACTGTTCCAGCGCAGCGCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCT  >  NC_002947/460746‑461037
                                                                                                                                                  |                                                                                                                                                 
cccAGGGCACTGTTCCAGCGCAGCGCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTAc                                                                                                                                                 >  3:233598/1‑149 (MQ=255)
 ccAGGGCACTGTTCCAGCGCAGCGCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTAcc                                                                                                                                                >  5:25049/1‑149 (MQ=255)
  cAGGGCACTGTTCCAGCGCAGCTCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTAccc                                                                                                                                               <  4:233598/149‑1 (MQ=255)
           gTTCCAGCGCAGCGCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACcgcg                                                                                                                                      >  6:268153/1‑149 (MQ=255)
                agcgcagcgcCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTgg                                                                                                                                 >  6:174990/1‑149 (MQ=255)
                agcgcagcgcCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTgg                                                                                                                                 >  4:58022/1‑149 (MQ=255)
                  cgcagcgcCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCa                                                                                                                               <  3:58022/149‑1 (MQ=255)
                    cagcgcCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGt                                                                                                                             <  8:185348/149‑1 (MQ=255)
                        gcCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCt                                                                                                                         >  8:112552/1‑149 (MQ=255)
                           ctaGGCCTTTAGCGCGGTATCCTCGTAGCCTGCGGGCATGCCATGCCACTCGGTGTGGAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGTGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGa                                                                                                                      <  8:174073/147‑1 (MQ=255)
                              ggCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCa                                                                                                                   <  8:36774/149‑1 (MQ=255)
                                    tAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTCCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGcc                                                                                                              <  1:185201/148‑1 (MQ=255)
                                          ggTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATgg                                                                                                       <  7:54802/149‑1 (MQ=255)
                                                 tCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAg                                                                                                >  7:85890/1‑149 (MQ=255)
                                                  cGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTGGGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAgg                                                                                               >  6:97170/1‑149 (MQ=255)
                                                     aGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATgg                                                                                                       >  8:285488/1‑138 (MQ=255)
                                                     aGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATgg                                                                                                       <  7:285488/138‑1 (MQ=255)
                                                       ccTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCgg                                                                                          <  2:308538/149‑1 (MQ=255)
                                                        cTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCggg                                                                                         >  7:24619/1‑149 (MQ=255)
                                                         cgCGGCCATGCCATGCCACTCGGGGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCgggg                                                                                        <  6:177585/148‑1 (MQ=255)
                                                             gccatgccatGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAg                                                                                    >  8:205678/1‑149 (MQ=255)
                                                                    catgccaCTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTAc                                                                                                                                                 >  8:146479/1‑81 (MQ=255)
                                                                    catgccaCTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTAc                                                                                                                                                 <  7:146479/81‑1 (MQ=255)
                                                                              ggTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGcagc                                                                    >  1:20991/1‑148 (MQ=255)
                                                                              ggTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGcagc                                                                    <  5:268153/148‑1 (MQ=255)
                                                                                   gCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCaa                                                              >  7:283963/1‑149 (MQ=255)
                                                                                    cAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAgc                                                                                   <  8:124434/127‑1 (MQ=255)
                                                                                    cAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAgc                                                                                   >  7:124434/1‑127 (MQ=255)
                                                                                     aGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACt                                                            >  5:169871/1‑149 (MQ=255)
                                                                                     aGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGAAGGGCAGCCAGCAACt                                                            >  6:242548/1‑149 (MQ=255)
                                                                                       ccACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTgg                                                          <  7:205678/149‑1 (MQ=255)
                                                                                       ccACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTgg                                                          >  7:278689/1‑149 (MQ=255)
                                                                                       ccACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTgg                                                          <  6:25049/149‑1 (MQ=255)
                                                                                        cacaGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGc                                                         <  8:283963/149‑1 (MQ=255)
                                                                                        cacaGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGc                                                         <  7:112552/149‑1 (MQ=255)
                                                                                           aGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAgg                                                      <  5:174990/149‑1 (MQ=255)
                                                                                            ggCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGt                                                     <  6:233478/149‑1 (MQ=255)
                                                                                            ggCTGGCGAGTTGCTGCTGGGTCCGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTGTGGCAGTGGCTTGAGCAGGGCCCTGCTGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGt                                                     <  5:97170/149‑1 (MQ=255)
                                                                                             gCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTg                                                    >  8:171466/1‑149 (MQ=255)
                                                                                               tGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGcagcca                                                                  <  8:164985/133‑1 (MQ=255)
                                                                                               ggTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGcagcca                                                                  >  7:164985/2‑133 (MQ=255)
                                                                                                  ctaGTTGCTGCTTGGTCAGCGTGCAGGCAGGCTCGGCTTGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGGAGGGCCCTGATGGGGATCATGGCCTGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCtt                                                <  4:157399/146‑1 (MQ=255)
                                                                                                    aGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAg                                             >  8:90730/1‑149 (MQ=255)
                                                                                                     gTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCtt                                                >  3:157399/1‑145 (MQ=255)
                                                                                                        gctgctGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCAt                                          >  6:111203/1‑148 (MQ=255)
                                                                                                            ctgGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCCACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATcaagg                                     >  5:5641/1‑149 (MQ=255)
                                                                                                               ggTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATcaaggcca                                  <  7:90730/149‑1 (MQ=255)
                                                                                                                         caggcaggCACCGCTGGGGGCAGGCCACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGtt                         <  6:5641/148‑1 (MQ=255)
                                                                                                                          aggcaggcACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTc                        >  7:99522/1‑148 (MQ=255)
                                                                                                                          aggcaggcACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTc                        >  4:2552/1‑148 (MQ=255)
                                                                                                                              aggcaACGCTGGGGGCAGGCTACCCCGTAACGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACAGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTg                   >  4:159045/1‑149 (MQ=255)
                                                                                                                                      cTGGGGGCAGGCCACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCgcag            >  2:184371/1‑148 (MQ=255)
                                                                                                                                         ggggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCAct        >  4:268389/1‑149 (MQ=255)
                                                                                                                                           ggCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgc      >  7:173812/1‑149 (MQ=255)
                                                                                                                                              aGGCCACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctgc   <  1:184371/149‑1 (MQ=255)
                                                                                                                                               ggCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCActgctgct  <  8:85890/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CCCAGGGCACTGTTCCAGCGCAGCGCCCAAGGCCTTTAGCGCGGTATCCTCGAAGCCTGCGGCCATGCCATGCCACTCGGTGTGCAGCCACAGGCTGTCGAGTTGCTGCTGGGTCAGCGTGCAGGCAGGCACCGCTGGGGGCAGGCTACCCCGTACCGCGTCTGGCAGTTGCTTGAGCAGGGCCCTGATGGGGATCAGGGCCGGGGGCAGCGCCGTGCAGGGCAGCCAGCAACTGGCAGGTGGCTTGAGCATCAAGGCCAAGGCTTGTTCCACTGCCCGCAGCACTGCTGCT  >  NC_002947/460746‑461037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑