Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,001,378 C→T 5.6% R308Q (CGG→CAG)  amaA ← L‑pipecolate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,001,3780CT 5.6% 210.4 / 2.5 71R308Q (CGG→CAG) amaAL‑pipecolate oxidase
Reads supporting (aligned to +/- strand):  ref base C (33/34);  new base T (2/2);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

GAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATAGTAA  >  NC_002947/6001233‑6001524
                                                                                                                                                 |                                                                                                                                                  
gAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCgg                                                                                                                                                  <  1:269434/148‑1 (MQ=255)
gAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCgg                                                                                                                                                  <  5:125030/148‑1 (MQ=255)
gAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCg                                                                                                                                                   <  6:116912/147‑1 (MQ=255)
     ggCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGAGCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAgg                                                                                                                                            <  4:234315/149‑1 (MQ=255)
       cgaaTCAGGTGGTCAGGCGTGAAGTCGATGCAGCCGGTCCTGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCATGCt                                                                                                                                          <  4:207690/146‑1 (MQ=255)
        gcATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTg                                                                                                                                         <  4:205366/149‑1 (MQ=255)
        gcATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTg                                                                                                                                         >  7:177203/1‑149 (MQ=255)
             aGGTGGTCAGGGGTTAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGa                                                                                                                                     <  2:162735/148‑1 (MQ=255)
                       gggTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAggcgg                                                                                                                          >  4:68555/1‑149 (MQ=255)
                       gggTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAggcgg                                                                                                                          <  8:177203/149‑1 (MQ=255)
                         gTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGcc                                                                                                                        <  8:139318/149‑1 (MQ=255)
                              gTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGAt                                                                                                                   >  7:246362/1‑149 (MQ=255)
                                 gATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGAtcgt                                                                                                                >  6:9554/1‑149 (MQ=255)
                                  aTGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGAtcgtc                                                                                                               <  5:109257/149‑1 (MQ=255)
                                      aGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAggcggc                                                                                                                         <  2:281883/135‑1 (MQ=255)
                                      aGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAggcggc                                                                                                                         >  1:281883/1‑135 (MQ=255)
                                       gCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGCAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCTGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCgcggc                                                                                                          >  3:281433/1‑149 (MQ=255)
                                       gCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGCAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCTGGCCCAGGCTGCCGAGCAGCAGGCGGACCTGATCGTCgcggc                                                                                                          >  3:285964/1‑149 (MQ=255)
                                         cGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCg                                                                                                                                      <  2:258354/119‑1 (MQ=255)
                                         cGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCg                                                                                                                                      >  1:258354/1‑119 (MQ=255)
                                            tCCATTGCATTTCCCACTCGACCTTGCCCATCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGAtg                                                                                                     <  8:125089/149‑1 (MQ=255)
                                                gTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGAt                                                                                                                   >  3:284151/1‑131 (MQ=255)
                                                gTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGAt                                                                                                                   >  5:251517/1‑131 (MQ=255)
                                                 tGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATgctgct                                                                                                >  6:144065/1‑149 (MQ=255)
                                                    aTTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAg                                                                                             <  2:35812/149‑1 (MQ=255)
                                                     tttCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAg                                                                                             >  1:211973/1‑148 (MQ=255)
                                                      ttCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCa                                                                                           >  6:170064/1‑149 (MQ=255)
                                                       tCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCAc                                                                                          >  7:78186/1‑149 (MQ=255)
                                                        cccACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACg                                                                                         >  4:260174/1‑149 (MQ=255)
                                                          cACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGt                                                                                       >  1:58479/1‑149 (MQ=255)
                                                               gACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCggg                                                                                  >  5:46745/1‑149 (MQ=255)
                                                               gACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCgg                                                                                   <  3:260174/148‑1 (MQ=255)
                                                               gACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCgg                                                                                   <  5:144065/148‑1 (MQ=255)
                                                                  cTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTAt                                                                               >  7:250304/1‑149 (MQ=255)
                                                                       ccAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCAc                                                                          <  5:170064/149‑1 (MQ=255)
                                                                         aGCTCCGGGCAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCTGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGa                                                                        <  4:281433/149‑1 (MQ=255)
                                                                         aGCTCCGGGCAATAGTGGCGCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCTGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGATGCTGAGCACGGTGCGGGTATCCCACGa                                                                        <  4:285964/149‑1 (MQ=255)
                                                                              cGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACAtt                                                                   >  2:17659/1‑149 (MQ=255)
                                                                               gggTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTg                                                                  <  8:246362/149‑1 (MQ=255)
                                                                               gggTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTAGACCCGGCCCAGGCTCCagag                                                                                                                                    >  5:164555/1‑83 (MQ=255)
                                                                                ggTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTgg                                                                 <  3:68555/149‑1 (MQ=255)
                                                                                     tAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATAGTCGCGGCGGATGCTGCTGAGCACGGTGAGGGTATCCCACGAACCTTgcacatt                                                            >  8:253864/1‑143 (MQ=255)
                                                                                      aGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCAtgt                                                           >  3:112945/1‑149 (MQ=255)
                                                                                        tGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACg                                                                                         <  1:220122/117‑1 (MQ=255)
                                                                                        tGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACg                                                                                         >  2:220122/1‑117 (MQ=255)
                                                                                        tGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTgg                                                         <  8:250304/149‑1 (MQ=255)
                                                                                                gATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTAcc                                                  <  6:46745/148‑1 (MQ=255)
                                                                                                gATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTAc                                                   <  5:9554/147‑1 (MQ=255)
                                                                                                   gCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTCCCTTGt                                              >  3:8870/1‑149 (MQ=255)
                                                                                                     gATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCg                                            >  2:237608/1‑149 (MQ=255)
                                                                                                       tCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTg                                                                  >  7:209077/1‑125 (MQ=255)
                                                                                                       tCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTg                                                                  <  8:209077/125‑1 (MQ=255)
                                                                                                          gCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGc                                       >  4:192085/1‑149 (MQ=255)
                                                                                                          gCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGc                                       >  2:56898/1‑149 (MQ=255)
                                                                                                            ccAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGa                                     <  4:112945/149‑1 (MQ=255)
                                                                                                               gCTGCGCACGTACCAGGCTGGCTTGTTGTCGACCCGGCCCAGTCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTTAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGc                                  <  7:253864/149‑1 (MQ=255)
                                                                                                               gCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGc                                  <  3:138374/149‑1 (MQ=255)
                                                                                                                     cACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTg                              >  6:267159/1‑147 (MQ=255)
                                                                                                                           ccAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGtt                      >  7:275472/1‑149 (MQ=255)
                                                                                                                                ctggctggTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAg                 <  2:235160/149‑1 (MQ=255)
                                                                                                                                   gctTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGc               >  2:5031/1‑148 (MQ=255)
                                                                                                                                     ttgttgTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGc                                                        <  1:211067/105‑1 (MQ=255)
                                                                                                                                     ttgttgTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGc                                                        >  2:211067/1‑105 (MQ=255)
                                                                                                                                     ttgttgTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTACTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGa                  <  6:236469/143‑1 (MQ=255)
                                                                                                                                     ttgttgTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTACTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGa                  >  5:236469/1‑143 (MQ=255)
                                                                                                                                       gttgtCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCGGCGGTTTGGAGGCTACCt          >  8:37505/1‑149 (MQ=255)
                                                                                                                                       gttgtCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTAcc           <  2:58479/148‑1 (MQ=255)
                                                                                                                                       gttgtCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCt          >  4:115924/1‑149 (MQ=255)
                                                                                                                                          gtCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGa        <  3:115924/148‑1 (MQ=255)
                                                                                                                                           tCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATa      <  3:192085/149‑1 (MQ=255)
                                                                                                                                           tCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATa      <  8:78186/149‑1 (MQ=255)
                                                                                                                                               cccGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACATTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGTGGCTACCTGATAGtaa  <  1:17659/149‑1 (MQ=255)
                                                                                                                                                ccGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATAGtaa  >  4:150129/1‑148 (MQ=255)
                                                                                                                                                ccGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATAGtaa  >  2:168410/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GAACAGGCGCATCAGGTGGTCAGGGGTGAAGTCGATGCAGCCGGTCCAGTGCATTTCCCACTCGACCTTGCCCAGCTCCGGGTAATAGTGGCTCTGGATGCGATCGGCCCAGCTGCGCACGAACCAGGCTGGCTTGTTGTCGACCCGGCCCAGGCTGCCGAGCAGCAGGCGGCCCTGATCGTCGCGGCGGATGCTGCTGAGCACGGTGCGGGTATCCCACGAACCTTGGCCATGTGGCAGTACCTTGTCGGCTGCGATGCCCTGCAGCGGTTTGGAGGCTACCTGATAGTAA  >  NC_002947/6001233‑6001524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑