Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,013,838	A→G	13.3%	intergenic (+43/+67)	xpt → / ← PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,013,838	0	A	G	13.3%	93.2 / 6.6	40	intergenic (+43/+67)	xpt/PP_5266	xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base A (12/21);  new base G (2/3);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.66e-01

GTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013693‑6013977                                                                                                                                                   |                                                                                                                                            gTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTgcagca                                                                                                                                          <  1:286827/149‑1 (MQ=255)     cAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAgg                                                                                                                                      >  1:183399/1‑149 (MQ=255)      aGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGt                                                                                                                                     >  1:204644/1‑149 (MQ=255)      aGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGt                                                                                                                                     >  7:138690/1‑149 (MQ=255)            gcCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGaa                                                                                                                               <  4:138203/149‑1 (MQ=255)                   cGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                        <  2:204644/149‑1 (MQ=255)                    gAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGc                                                                                                                       <  3:149449/149‑1 (MQ=255)                          gACAGCCAGGGTTACCGCGTTGAATCGCCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgt                                                                                                                         >  1:175136/1‑142 (MQ=255)                          gACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgt                                                                                                                         <  2:175136/141‑1 (MQ=255)                               ccAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTgg                                                                                                            <  8:3398/149‑1 (MQ=255)                                 aGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGa                                                                                                          <  5:259517/149‑1 (MQ=25)                                     ttACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCgg                                                                                                      <  1:296415/149‑1 (MQ=21)                                        ccGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGc                                                                                                    <  8:138690/148‑1 (MQ=255)                                         cgcgTTTAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGt                                                                                                  <  5:108302/149‑1 (MQ=255)                                         cgcgTTGAATCG‑CTGGCCCGGGTGAAGTCGCGGGAAGGTGGTGTGGTCAGCTTCATCGAATAACCGCGACACCTGCTGCGCGGGCACGCCCGTTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGt                                                                                                  <  1:239058/149‑1 (MQ=255)                                           cgTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCACCCCCAGCTTCAGCACACGGCTCCAAACCAGTGCCGTACCTGAGCGACCCGGCGTGc                                                                                                >  3:272273/1‑149 (MQ=255)                                                  tCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCCCTACCACCGGTGCAGCACAGGTGTGGAACGCGGTGGCGTAACGGTGGGAGCGGGCGTGGCcgcgca                                                                                         >  7:42416/1‑147 (MQ=255)                                                         gCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGcc                                                                                    <  2:183399/148‑1 (MQ=255)                                                                tGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCt                                                                            <  3:98128/149‑1 (MQ=255)                                                                    gTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGACCGCCCGCTCCCACAGGGTCAGCCCAGGGATCGCAAGCGGTGGCGGACCTGTGTGTGCGGGCGTGGCCGCGAAAGGCCGCAAACCgtttt                                                                        >  8:136333/1‑148 (MQ=255)                                                                       gCTCGAATGTGGTGTGGTCAGCTTCCTCGAAAAAACCCGACACCTGCTACGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                     <  7:136333/149‑1 (MQ=37)                                                                        cgggACGGGGGTGTGGCCAGCTTCATCCAAAAAACGCGACACCTGCTTCGCGGGCCCGCCCGCTCCCCCCGGGGCAGCACAGGCCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                    <  7:249297/147‑1 (MQ=25)                                                                                 ggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGc                                                                                                    >  8:278437/1‑108 (MQ=14)                                                                                 ggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGc                                                                                                    <  7:278437/108‑1 (MQ=14)                                                                                     tgttCAGCTTCATCTAATAAACGCTACACCTGCTTCGCGGGCACGCCCGCTCCCACGGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                       <  8:42416/146‑1 (MQ=38)                                                                                      ggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGGCGCCCCGGGCTGGCACGCGGTGGCGGTCCTGTGGGGGCGGGCGGGCCCGCGAATGGGCGCCACCCGGTTATATGGTTGGCGGCAGccc                                                      >  2:83753/1‑149 (MQ=19)                                                                                            cTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                   <  1:97220/146‑1 (MQ=255)                                                                                            cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTACCACAGGTGCAGCAAAGGTATCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCATTTATCTGGTTGCCGCCAGCCCCGCcaa                                                >  7:1146/1‑149 (MQ=38)                                                                                            cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCAACCACACGTGCCGCACAGGTCTAGCACCCGGTGGCGGACCGGTGGGAGCGGGGGGGCCCGCGCAAGGCCGCAACCCGTTTATCTGGTTTGCGCccccccccccca                                                >  8:171760/1‑147 (MQ=255)                                                                                                aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaa                                             >  3:194494/1‑148 (MQ=255)                                                                                                aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCAGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaa                                             >  5:143646/1‑148 (MQ=255)                                                                                                                acCTGCTTCGCGGGCACGGGCGCTCCCTCTGGTGCAGCACAGGTCTCGATCGCTGTGCCGTACCTGTGTGAGCGGGCGTGCCCGCGAATGGCCTCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGtt                            <  4:272273/149‑1 (MQ=38)                                                                                                                      ttCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                      <  4:194494/149‑1 (MQ=255)                                                                                                                                 gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           <  7:40251/149‑1 (MQ=255)                                                                                                                                 gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           <  7:75076/149‑1 (MQ=255)                                                                                                                                 gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc           >  8:75076/1‑149 (MQ=255)                                                                                                                                       gcccACCGGTGCAGCCCAGGTCTCGACCGCTGTGCCGTACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc     <  1:128476/148‑1 (MQ=255)                                                                                                                                        cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc    >  5:94799/1‑149 (MQ=21)                                                                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc   <  3:141745/149‑1 (MQ=255)                                                                                                                                         ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc    <  7:171760/148‑1 (MQ=255)                                                                                                                                           acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc  >  4:171637/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                            GTTCCAGGGCGGCCGTGCCGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCC  >  NC_002947/6013693‑6013977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑