Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,013,854 G→C 17.1% intergenic (+59/+51) xpt → / ← PP_5266 xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,013,8540GC17.1% 95.9 / 6.6 39intergenic (+59/+51)xpt/PP_5266xanthine phosphoribosyltransferase/acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (11/21);  new base C (4/3);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGG  >  NC_002947/6013711‑6013998
                                                                                                                                                |                                                                                                                                                
cGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgtg                                                                                                                                             <  2:204644/149‑1 (MQ=255)
 gAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGc                                                                                                                                            <  3:149449/149‑1 (MQ=255)
       gACAGCCAGGGTTACCGCGTTGAATCGCCTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgt                                                                                                                                              >  1:175136/1‑142 (MQ=255)
       gACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCtgt                                                                                                                                              <  2:175136/141‑1 (MQ=255)
            ccAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTgg                                                                                                                                 <  8:3398/149‑1 (MQ=255)
              aGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGa                                                                                                                               <  5:259517/149‑1 (MQ=25)
                  ttACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCgg                                                                                                                           <  1:296415/149‑1 (MQ=21)
                     ccGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGc                                                                                                                         <  8:138690/148‑1 (MQ=255)
                      cgcgTTTAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGt                                                                                                                       <  5:108302/149‑1 (MQ=255)
                      cgcgTTGAATCG‑CTGGCCCGGGTGAAGTCGCGGGAAGGTGGTGTGGTCAGCTTCATCGAATAACCGCGACACCTGCTGCGCGGGCACGCCCGTTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGt                                                                                                                       <  1:239058/149‑1 (MQ=255)
                        cgTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCACCCCCAGCTTCAGCACACGGCTCCAAACCAGTGCCGTACCTGAGCGACCCGGCGTGc                                                                                                                     >  3:272273/1‑149 (MQ=255)
                               tCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCCCTACCACCGGTGCAGCACAGGTGTGGAACGCGGTGGCGTAACGGTGGGAGCGGGCGTGGCcgcgca                                                                                                              >  7:42416/1‑147 (MQ=255)
                                      gCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGcc                                                                                                         <  2:183399/148‑1 (MQ=255)
                                             tGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCt                                                                                                 <  3:98128/149‑1 (MQ=255)
                                                 gTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGACCGCCCGCTCCCACAGGGTCAGCCCAGGGATCGCAAGCGGTGGCGGACCTGTGTGTGCGGGCGTGGCCGCGAAAGGCCGCAAACCgtttt                                                                                             >  8:136333/1‑148 (MQ=255)
                                                    gCTCGAATGTGGTGTGGTCAGCTTCCTCGAAAAAACCCGACACCTGCTACGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCt                                                                                          <  7:136333/149‑1 (MQ=37)
                                                     cgggACGGGGGTGTGGCCAGCTTCATCCAAAAAACGCGACACCTGCTTCGCGGGCCCGCCCGCTCCCCCCGGGGCAGCACAGGCCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTg                                                                                         <  7:249297/147‑1 (MQ=25)
                                                              ggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGc                                                                                                                         >  8:278437/1‑108 (MQ=14)
                                                              ggtgtggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGc                                                                                                                         <  7:278437/108‑1 (MQ=14)
                                                                  tgttCAGCTTCATCTAATAAACGCTACACCTGCTTCGCGGGCACGCCCGCTCCCACGGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGcc                                                                            <  8:42416/146‑1 (MQ=38)
                                                                   ggtCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGGCGCCCCGGGCTGGCACGCGGTGGCGGTCCTGTGGGGGCGGGCGGGCCCGCGAATGGGCGCCACCCGGTTATATGGTTGGCGGCAGccc                                                                           >  2:83753/1‑149 (MQ=19)
                                                                         cTTCATCGAATAACCGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACCGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGc                                                                        <  1:97220/146‑1 (MQ=255)
                                                                         cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTACCACAGGTGCAGCAAAGGTATCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCATTTATCTGGTTGCCGCCAGCCCCGCcaa                                                                     >  7:1146/1‑149 (MQ=38)
                                                                         cTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACCCCCCCAACCACACGTGCCGCACAGGTCTAGCACCCGGTGGCGGACCGGTGGGAGCGGGGGGGCCCGCGCAAGGCCGCAACCCGTTTATCTGGTTTGCGCccccccccccca                                                                     >  8:171760/1‑147 (MQ=255)
                                                                             aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaa                                                                  >  3:194494/1‑148 (MQ=255)
                                                                             aTCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCAGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCcaacaa                                                                  >  5:143646/1‑148 (MQ=255)
                                                                                             acCTGCTTCGCGGGCACGGGCGCTCCCTCTGGTGCAGCACAGGTCTCGATCGCTGTGCCGTACCTGTGTGAGCGGGCGTGCCCGCGAATGGCCTCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGtt                                                 <  4:272273/149‑1 (MQ=38)
                                                                                                   ttCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTcttctt                                           <  4:194494/149‑1 (MQ=255)
                                                                                                              gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                <  7:40251/149‑1 (MQ=255)
                                                                                                              gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                <  7:75076/149‑1 (MQ=255)
                                                                                                              gCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGc                                >  8:75076/1‑149 (MQ=255)
                                                                                                                    gcccACCGGTGCAGCCCAGGTCTCGACCGCTGTGCCGTACCGGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGc                          <  1:128476/148‑1 (MQ=255)
                                                                                                                     cccACAGGTACGGCACAGCGTTCGAGACCTGTGCTGCACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                         >  5:94799/1‑149 (MQ=21)
                                                                                                                      ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGccc                        <  3:141745/149‑1 (MQ=255)
                                                                                                                      ccACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcc                         <  7:171760/148‑1 (MQ=255)
                                                                                                                        acaGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGcccc                       >  4:171637/1‑148 (MQ=255)
                                                                                                                                            gAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTgg  >  1:9255/1‑149 (MQ=255)
                                                                                                                                             aaCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                   >  6:294466/1‑131 (MQ=255)
                                                                                                                                             aaCGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCCGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACt                   <  5:294466/131‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CGAACTGGACAGCCAGGGTTACCGCGTTGAATCG‑CTGGCCCGGGTGAAGTCGCTGGAAGGTGGTGTGGTCAGCTTCATCGAATAAACGCGACACCTGCTTCGCGGGCACGCCCGCTCCCACAGGTGCAGCACAGGTCTCGAACGCTGTGCCGTACCTGTGGGAGCGGGCGTGCCTGCGAATGGCCGCAACCCTTTTATCTGGTTGCCGCCAGCCCCGCCAACAACAAGCGCTGGTAAAGTTCTTCTTTAAGCCCCTGCGGCTGCCCCAACTGCATCCGCCCCAGGTGG  >  NC_002947/6013711‑6013998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑