Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,018,374	A→C	5.4%	Y276D (TAC→GAC)	dadX ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,018,374	0	A	C	5.4%	238.3 / 3.3	74	Y276D (TAC→GAC)	dadX	alanine racemase
Reads supporting (aligned to +/- strand):  ref base A (35/35);  new base C (2/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGAATTCGACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGTTGCAGGCGTGC  >  NC_002947/6018232‑6018521                                                                                                                                                 |                                                                                                                                                     cAGAATTCGACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑a                                                                                                                                                      <  3:114828/143‑1 (MQ=255) cAGAAATCGACAGGCCTGCCGACAGAGGCATGCGGTACGTCGGTGAGGTCGATGATGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACAGGGGTGCCGTTAGGCGCCTGGCGCCGGA‑‑Agagaac                                                                                                                                                >  4:114828/1‑144 (MQ=255)     aTTCGACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑CGCCATGGGc                                                                                                                                             >  1:289415/1‑148 (MQ=255)     aTTCGACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCa                                                                                                                                            <  2:132283/149‑1 (MQ=255)         gACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGc                                                                                                                                        >  5:140758/1‑149 (MQ=255)              ggCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CGATg                                                                                                                                  <  2:216274/149‑1 (MQ=255)               gCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATgg                                                                                                                                 <  8:77499/149‑1 (MQ=255)                cTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCTACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGc                                                                                                                                <  6:165342/149‑1 (MQ=255)                   ccGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGCTCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGAc                                                                                                                             <  7:272529/149‑1 (MQ=255)                   ccGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGAc                                                                                                                             >  4:49008/1‑149 (MQ=255)                    cGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑CGCCATGGGCTATGC‑CGATGGCTAcc                                                                                                                            <  4:94240/149‑1 (MQ=255)                     gACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑CGCCATGGGCTATGC‑CGATGGCTAccc                                                                                                                           <  2:289415/149‑2 (MQ=255)                     gACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACca                                                                                                                           >  8:58334/1‑149 (MQ=255)                        aGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGCCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCacac                                                                                                                        <  6:82814/149‑1 (MQ=255)                        aGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGCCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCacac                                                                                                                        >  5:82814/1‑149 (MQ=255)                          tGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACAccc                                                                                                                      <  2:253761/149‑1 (MQ=255)                                  ggTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGt                                                                                                              >  2:29736/1‑149 (MQ=255)                                                gTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGa                                                                                                <  7:87636/149‑1 (MQ=255)                                                gTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGa                                                                                                <  5:84666/149‑1 (MQ=255)                                                        tGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTg                                                                                        >  4:158605/1‑149 (MQ=255)                                                         gAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTgg                                                                                       >  6:101468/1‑149 (MQ=255)                                                           gCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCa                                                                                     <  4:289599/149‑1 (MQ=255)                                                             aTGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCAc                                                                                    >  6:249651/1‑148 (MQ=255)                                                                  cATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACCCCCACGCGGGTCGGCCGCGGGCTGACGAACTTGGCACCATCGc                                                                              >  4:181549/1‑149 (MQ=255)                                                                   aTCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGcc                                                                             <  1:29736/149‑1 (MQ=255)                                                                    tCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGcc                                                                             >  5:117623/1‑148 (MQ=255)                                                                      gACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACTGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAAc                                                                          >  1:218964/1‑149 (MQ=255)                                                                      gACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAAc                                                                          <  5:249651/149‑1 (MQ=255)                                                                       acacCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGTGAAGCCATCGGCATAGCACCATGGCGACCACACCCACGCGGGTCGGCCGAGGGGTGACGAAATTGGCACCATAGCCa                                                                            >  1:95101/1‑149 (MQ=255)                                                                         acCCGGCCTATCAGCTGGGTGCGCTTGCCATCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCgg                                                                       <  8:289928/149‑1 (MQ=255)                                                                              gCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCacac                                                                                                                        >  4:243484/1‑95 (MQ=255)                                                                              gCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCacac                                                                                                                        <  3:243484/95‑1 (MQ=255)                                                                                  aTCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCgg                                                               >  3:45607/1‑148 (MQ=255)                                                                                   tCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCgg                                                               >  3:290050/1‑147 (MQ=255)                                                                                   tCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCgg                                                               >  1:196537/1‑147 (MQ=255)                                                                                       cTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTc                                                                                                             >  6:261384/1‑97 (MQ=255)                                                                                       cTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTc                                                                                                             <  5:261384/97‑1 (MQ=255)                                                                                        tGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGgcaggcag                                                        >  8:241294/1‑149 (MQ=255)                                                                                         gggTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGc                                                       >  2:42860/1‑149 (MQ=255)                                                                                         gggTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGc                                                       >  7:112788/1‑149 (MQ=255)                                                                                               gcTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCgcgc                                                 >  4:22208/1‑149 (MQ=255)                                                                                                    ccAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCAcgcg                                                                                                                 <  8:111625/80‑1 (MQ=255)                                                                                                    ccAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCAcgcg                                                                                                                 >  7:111625/1‑80 (MQ=255)                                                                                                    ccAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGc                                             <  3:158605/148‑1 (MQ=255)                                                                                                     cAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTg                                           <  3:22208/149‑1 (MQ=255)                                                                                                          aCCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCAc                                      >  3:185888/1‑149 (MQ=255)                                                                                                             aGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcg                                   <  6:140758/149‑1 (MQ=255)                                                                                                              gCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcgc                                  >  1:73194/1‑149 (MQ=255)                                                                                                              gCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcgc                                  >  7:2871/1‑149 (MQ=255)                                                                                                              gCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcgc                                  >  7:263516/1‑149 (MQ=255)                                                                                                               cACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCgg                                                               >  5:232457/1‑119 (MQ=255)                                                                                                               cACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCgg                                                               <  6:232457/119‑1 (MQ=255)                                                                                                                  cGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGAtt                              >  4:236922/1‑149 (MQ=255)                                                                                                                    gggTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGc                            <  8:112788/149‑1 (MQ=255)                                                                                                                    gggTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGc                            <  3:49008/149‑1 (MQ=255)                                                                                                                      gTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAg                          <  6:160092/149‑1 (MQ=255)                                                                                                                       tGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAgg                         <  7:58334/149‑1 (MQ=255)                                                                                                                       tGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAgg                         <  8:263516/149‑1 (MQ=255)                                                                                                                       tGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAgg                         <  8:67572/149‑1 (MQ=255)                                                                                                                       tGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAgg                         <  7:241294/149‑1 (MQ=255)                                                                                                                       tGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAgg                         <  5:101468/149‑1 (MQ=255)                                                                                                                        gCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcgc                                  >  3:227187/1‑139 (MQ=255)                                                                                                                        gCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCcgc                                  <  4:227187/139‑1 (MQ=255)                                                                                                                         ccGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGt                       <  8:28063/149‑1 (MQ=255)                                                                                                                           gTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACg                                              >  6:22347/1‑124 (MQ=255)                                                                                                                           gTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACg                                              <  5:22347/124‑1 (MQ=255)                                                                                                                                gcgcCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCAc                 <  2:300507/148‑1 (MQ=255)                                                                                                                                gcgcCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCAc                 >  1:300507/1‑148 (MQ=255)                                                                                                                                  gcCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCgg              >  5:278127/1‑149 (MQ=255)                                                                                                                                  gcCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCgg              <  3:236922/149‑1 (MQ=255)                                                                                                                                   ccTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGt             >  4:293707/1‑149 (MQ=255)                                                                                                                                    cTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGtt            >  5:872/1‑149 (MQ=255)                                                                                                                                       gcgcGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGTTGc          <  6:278127/148‑1 (MQ=255)                                                                                                                                        gtgtggT‑‑CGCCATGGGCTATGC‑CGATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGTTGCAg        >  2:45469/5‑149 (MQ=255)                                                                                                                                              t‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGTTGCAGGCGTGc  <  6:872/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                     CAGAATTCGACCGGGCTGCCGACAGTGGCTTGCGGTACGTCGGTGAGGTCGATGCTGAGCATGTCCATCGACACCCGGCCGATCAGCTGGGTGCGCTTGCCAGCGACCAGCACCGGGGTGCCGTTAGGCGCCTGGCGCGGGT‑‑AGCCATCGGCATAGC‑CCATGGCGACCACACCCACGCGGGTCGGCCGAGGGCTGACGAACTTGGCACCATAGCCAACCGGCTCGCCGGCAGGCAGCTCGCGCACGCTGATCACCCGCGATTGCAGGGTCATCACCGGTTGCAGGCGTGC  >  NC_002947/6018232‑6018521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑