Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,019,275 G→C 10.6% intergenic (‑76/+69) dadX ← / ← dadA‑II alanine racemase/D‑amino acid:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,019,2750GC10.6% 152.7 / 8.2 56intergenic (‑76/+69)dadX/dadA‑IIalanine racemase/D‑amino acid:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base C (4/2);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.62e-01

CCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGAGATATCCAGGCCT  >  NC_002947/6019127‑6019422
                                                                                                                                                    |                                                                                                                                                    
ccGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTg                                                                                                                                                      <  5:223275/149‑1 (MQ=255)
  gTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGca                                                                                                                                                    <  3:165798/149‑1 (MQ=255)
          aCGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTg                                                                                                                                                      <  4:188287/139‑1 (MQ=255)
          aCGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTg                                                                                                                                                      >  3:188287/1‑139 (MQ=255)
          aCGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTc                                                                                                                                            <  7:130865/149‑1 (MQ=255)
                cAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGc                                                                                                                                      >  1:37536/1‑149 (MQ=255)
                cAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGc                                                                                                                                      >  2:85497/1‑149 (MQ=255)
                      gTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTTTGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCCCAGGTCGTGGACAGCTTTCAAAAGCggtggg                                                                                                                                >  7:179887/1‑148 (MQ=255)
                       tAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGgtggtg                                                                                                                               >  2:282191/1‑149 (MQ=255)
                         gTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGtt                                                                                                                             >  6:303314/1‑149 (MQ=255)
                               gcgcAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCtgtg                                                                                                                       >  6:172594/1‑149 (MQ=255)
                               gcgcAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCtgt                                                                                                                        <  5:200416/148‑1 (MQ=255)
                               gcgcAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCtgt                                                                                                                        <  2:37536/148‑1 (MQ=255)
                                   aGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAg                                                                                                                   <  3:129043/149‑1 (MQ=255)
                                       ccTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCggg                                                                                                               <  1:282191/149‑1 (MQ=255)
                                          ggAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTGCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCaa                                                                                                            <  1:232983/149‑1 (MQ=255)
                                           gAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAg                                                                                                           >  6:192602/1‑149 (MQ=255)
                                                  aTCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGAACACCACCGCTTTTGAAAGCTGTGCACGACCTGTGGGAGCg                                                                                                                 >  5:57631/1‑136 (MQ=17)
                                                  aTCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGAACACCACCGCTTTTGAAAGCTGTGCACGACCTGTGGGAGCg                                                                                                                 <  6:57631/136‑1 (MQ=17)
                                                     aGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGc                                                                                                              >  3:168071/1‑136 (MQ=255)
                                                     aGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGc                                                                                                              <  4:168071/136‑1 (MQ=255)
                                                     aGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAAt                                                                                                  <  5:172594/148‑1 (MQ=255)
                                                      gggCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCtgt                                                                                                                        >  8:237866/1‑125 (MQ=255)
                                                      gggCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCtgt                                                                                                                        <  7:237866/125‑1 (MQ=255)
                                                      gggCGCGGGCGGGACGCATAGCGGCTGCCTTATGGGGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACATGTCGTGCACAGCTTTCAAAAGCGGGGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATgg                                                                                                <  5:10583/149‑1 (MQ=255)
                                                       ggCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGAGGAGTTCCTGAGGGAGCGGGCAAGCCCGCAAATgcg                                                                                               >  4:270976/1‑147 (MQ=255)
                                                          gcgggcggGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTAAGGTGGGAGCGGGCAAGCCAGAAAATGGGcag                                                                                            >  8:136615/1‑147 (MQ=39)
                                                            gggcgggACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGc                                                                                          >  3:71655/1‑149 (MQ=255)
                                                            gggcgggACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGAGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCgg                                                                                           >  4:104289/1‑148 (MQ=255)
                                                               cgggACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGGGGATTATTCGCGGGCTTGCCCGCTCCCACAGGGCGGGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGcaca                                                                                       <  3:80962/149‑1 (MQ=255)
                                                                 gcaCGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAgg                                                                                     >  2:45504/3‑149 (MQ=255)
                                                                     gCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑t                                                                                >  5:235653/1‑149 (MQ=255)
                                                                        tAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑t                                                                                >  1:208949/1‑146 (MQ=255)
                                                                        tAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑t                                                                                <  2:208949/146‑1 (MQ=255)
                                                                           cGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTActgc                                                                          <  6:235653/149‑1 (MQ=255)
                                                                                 ccTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGGGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGa                                                                    <  3:104289/149‑1 (MQ=255)
                                                                                 ccTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTAGTGAACAGCTTTAAAAAGCGGTGGTGTTCCAGAGGGAGCGGGCAAGCCCGAAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTg                                                                     >  5:168717/1‑148 (MQ=38)
                                                                                  cTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAg                                                                   >  1:71436/1‑149 (MQ=255)
                                                                                   ttATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGc                                                                  >  6:66092/1‑149 (MQ=255)
                                                                                   ttATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGc                                                                  >  5:243939/1‑149 (MQ=255)
                                                                                        gtggtTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTTGCCCCACCCCCAAGTGGGGGAAAGCGTTAAAAAACGGTGGTGTTTCTGTGGGATCGGGCCACCCCCCAAATTGGCCCGGCCAGGCTGTTTAATG‑TGGTGAGCCGgcg                                                             >  2:287549/1‑149 (MQ=255)
                                                                                                    gAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGt                                                 >  4:198185/1‑149 (MQ=255)
                                                                                                     aaaTCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGt                                                 <  5:303314/148‑1 (MQ=255)
                                                                                                         cGGGGGTGGATTATTCGCGGGCTGGCCCGCCCCCCCAGGTCGGGCACCCCTTGCAAACGCGGGGGTGGTCCTGGGGGCGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGGGAGCCGGCGCGCTTTGCCCGTGCTTg                                            <  4:174337/149‑1 (MQ=255)
                                                                                                          ggtggtGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTgg                                           >  6:219869/1‑149 (MQ=255)
                                                                                                                  attattCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTcc                                   <  1:45504/149‑1 (MQ=255)
                                                                                                                   ttattCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTccc                                  >  1:56986/1‑149 (MQ=255)
                                                                                                                          cgGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTAtt                           <  1:85497/149‑1 (MQ=255)
                                                                                                                           ggcctgccccGCCCCCACCGGCCGGCCACAGATTTCAAACGCGGGGGGGTCCGGGGGGGCGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTg                          <  1:287549/142‑1 (MQ=255)
                                                                                                                                  cccGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAAc                   <  5:66092/149‑1 (MQ=255)
                                                                                                                                  cccGCTCCCACAGGAACACCACCGCTTTTGAAAGCTGTGCACGACCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAAc                   >  5:209710/1‑149 (MQ=18)
                                                                                                                                  cccGCTCCCACAGGAACACCACCGCTTTTAAAAGCTGTGCACGACCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAAc                   >  3:208876/1‑149 (MQ=18)
                                                                                                                                   ccGCTCCCACAGGAACACCACCGCTTTTGAAAGCTGTGCACGACCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGcaagaa                    >  1:262689/1‑142 (MQ=17)
                                                                                                                                      cTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGga               <  6:243939/149‑1 (MQ=255)
                                                                                                                                       tCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACgg                 >  7:155684/1‑146 (MQ=255)
                                                                                                                                         ccACAGGTCGTGCACAGCTTTCGAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGgaga             >  7:47211/1‑148 (MQ=255)
                                                                                                                                              ggTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCAGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGAGATATc         >  5:219708/1‑147 (MQ=255)
                                                                                                                                               ggcGTGCATAGTTTTCAATTGCGTTGGTGTTCGTGGGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGAGATATCCAg      <  8:179887/147‑1 (MQ=255)
                                                                                                                                               gTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGAGATATCCAg      <  3:198185/149‑1 (MQ=255)
                                                                                                                                                    gCACAGGTTTCAAATGCGGTGGTGTTCGTTTTGGTGGTGTCAAGCCCTCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCTCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGATATATCCAGGCCt  <  6:168717/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGTCAGTTCACGGGCCAGACGGTAGTTGTGGCGCAGGGCCTGGAGGTCGATCAGGGCGCGGGCGGGACGCATAGCGGCTGCCTTATGGTGGTTCGGGTTGAAATCGGTGGTGGATTATTCGCGGGCTTGCCCGCTCCCACAGGTCGTGCACAGCTTTCAAAAGCGGTGGTGTTCCTGTGGGAGCGGGCAAGCCCGCAAATGGGCCGGCACAGGCTG‑TTACTGCTGGTGAGCCGGCGCGCTTTGCCCGTGCTTGGCCACTTCCCGGCTATTGCCATAACGGGAGATATCCAGGCCT  >  NC_002947/6019127‑6019422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑