Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,023,159 G→A 7.7% intergenic (+74/+70) PP_5273 → / ← PP_5274 oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,023,1590GA 7.7% 190.0 / 4.1 65intergenic (+74/+70)PP_5273/PP_5274oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/35);  new base A (2/3);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

CTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTC  >  NC_002947/6023012‑6023300
                                                                                                                                                   |                                                                                                                                             
cTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGt                                                                                                                                              <  7:195412/149‑1 (MQ=255)
       cAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCt                                                                                                                                       >  1:149056/1‑149 (MQ=255)
         aGCCCCTGCGCGCGCCACTGCTGGCGCGGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGAAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCGcc                                                                                                                                     <  8:279748/149‑1 (MQ=255)
         aGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCTcc                                                                                                                                     <  3:87822/149‑1 (MQ=255)
            aCCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCca                                                                                                                                   >  2:281736/1‑148 (MQ=255)
                 cgcTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAgg                                                                                                                               <  5:239152/147‑1 (MQ=255)
                 cgcTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCACGCCCGCTCCCACAgg                                                                                                                               <  5:93113/147‑1 (MQ=255)
                  gcTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCTCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTc                                                                                                                            <  1:44651/149‑1 (MQ=255)
                      gCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGAGTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGt                                                                                                                         <  6:207093/148‑1 (MQ=255)
                      gCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTg                                                                                                                        <  2:214830/149‑1 (MQ=255)
                      gCCACTGCTCGCGCTTGCAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCCAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGt                                                                                                                         <  4:69570/148‑1 (MQ=255)
                                gcgcTGGGACTGCTGTGGCATCGCCTCAAAGAGCTGCCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCaa                                                                                                              <  2:233074/149‑1 (MQ=255)
                                  gcTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGt                                                                                                            >  4:230797/1‑149 (MQ=255)
                                      ggAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTgg                                                                                                        <  3:230797/149‑1 (MQ=255)
                                            cTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGaac                                                                                                   <  2:149056/148‑1 (MQ=255)
                                                 gCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAgg                                                                                             <  8:177463/149‑1 (MQ=255)
                                                 gCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAg                                                                                              <  7:207594/148‑1 (MQ=255)
                                                      gCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGc                                                                                            <  2:227069/145‑1 (MQ=255)
                                                      gCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGc                                                                                            >  1:227069/1‑145 (MQ=255)
                                                      gCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTc                                                                                        <  5:135294/149‑1 (MQ=255)
                                                         tCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGt                                                                                                                             <  5:313624/109‑1 (MQ=255)
                                                         tCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGt                                                                                                                             >  6:313624/1‑109 (MQ=255)
                                                         tCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAg                                                                                     >  1:108591/1‑149 (MQ=255)
                                                            aaGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGa                                                                                  <  2:139090/149‑1 (MQ=255)
                                                            aaGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGa                                                                                  >  1:139090/1‑149 (MQ=255)
                                                             agagCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGAc                                                                                 <  6:27405/149‑1 (MQ=255)
                                                                 cTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATc                                                                             >  7:175430/1‑149 (MQ=255)
                                                                 cTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATc                                                                             <  6:221193/149‑1 (MQ=255)
                                                                    aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                           <  4:39106/148‑1 (MQ=255)
                                                                     cTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                         <  1:281736/149‑1 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCaa                                                                                                              <  5:164924/111‑1 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCaa                                                                                                              >  6:164924/1‑111 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                         >  2:261780/1‑148 (MQ=255)
                                                                      ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCATGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                         >  4:295578/1‑148 (MQ=255)
                                                                        gAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                      >  6:40227/1‑149 (MQ=255)
                                                                        gAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                      >  3:195983/1‑149 (MQ=255)
                                                                         aaaGTCCCCATCCTTTTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCTCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGTAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                      <  7:293168/148‑1 (MQ=255)
                                                                              ccccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                >  6:220161/1‑149 (MQ=255)
                                                                               cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                <  7:30898/148‑1 (MQ=255)
                                                                                ccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                >  2:44219/1‑147 (MQ=255)
                                                                                  aTCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAg                                                             >  6:128837/1‑148 (MQ=255)
                                                                                      ttGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAggg                                                        >  2:38818/1‑149 (MQ=255)
                                                                                         tgGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAACGGttt                                                     <  7:281025/149‑1 (MQ=255)
                                                                                             agcaACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGc                                                 <  2:108591/149‑1 (MQ=255)
                                                                                               caACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCATGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGccc                                               <  3:295578/149‑1 (MQ=255)
                                                                                                  cTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                           <  2:285545/134‑1 (MQ=255)
                                                                                                  cTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                           >  1:285545/1‑134 (MQ=255)
                                                                                                     tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGAt                                         >  5:207046/1‑149 (MQ=255)
                                                                                                     tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGAt                                         >  5:202550/1‑149 (MQ=255)
                                                                                                          cTTAAAATCTAACAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCgg                                     <  7:270857/148‑1 (MQ=255)
                                                                                                           ttAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                   >  1:233341/1‑149 (MQ=255)
                                                                                                             aaaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                   <  5:128837/147‑1 (MQ=255)
                                                                                                              aaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCt                                 <  1:44219/148‑1 (MQ=255)
                                                                                                                 tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                         <  8:44835/105‑1 (MQ=255)
                                                                                                                 tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGAGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTc                             >  4:120753/1‑149 (MQ=255)
                                                                                                                  cTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                            >  7:284311/1‑149 (MQ=255)
                                                                                                                     accgCTGCCGCGCCCCCTGGGGGAGCGGGCGGGCCCGCTCCCACAGGGTCGGGGCAACTTTCAAGTCTGGCCACCAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAg                         <  8:175430/146‑1 (MQ=255)
                                                                                                                          tGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAg                    >  2:207424/1‑149 (MQ=255)
                                                                                                                              gcgcCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCg                >  2:228923/1‑149 (MQ=255)
                                                                                                                                     cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATcgc         <  5:40227/149‑1 (MQ=255)
                                                                                                                                         gggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa     >  1:132238/1‑149 (MQ=255)
                                                                                                                                          ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa     <  5:220161/148‑1 (MQ=255)
                                                                                                                                          ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc    <  1:228923/149‑1 (MQ=255)
                                                                                                                                          ggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc    >  4:97459/1‑149 (MQ=255)
                                                                                                                                            aGCGGGCGTGCCCGCTCCCACAGAGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTc  <  3:120753/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CTGGCGGCAAGCACCTGCGCTCGCCACTGCTGGCGCTGGGAATGCTGTGGCATCGCCTCAAAGAGCTGACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTC  >  NC_002947/6023012‑6023300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑