Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,023,226 G→T 8.7% intergenic (+141/+3) PP_5273 → / ← PP_5274 oxidoreductase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,023,2260GT 8.7% 136.5 / 3.1 46intergenic (+141/+3)PP_5273/PP_5274oxidoreductase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

ACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATGCAGCATCAGCCCCCAACGCCCCAAAC  >  NC_002947/6023080‑6023371
                                                                                                                                                  |                                                                                                                                                 
aCTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgt                                                                                                                                                  <  4:39106/148‑1 (MQ=255)
 cTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                <  1:281736/149‑1 (MQ=255)
  ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                >  2:261780/1‑148 (MQ=255)
  ttGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCATGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                >  4:295578/1‑148 (MQ=255)
    gAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                                                                                             >  6:40227/1‑149 (MQ=255)
    gAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                                                                                             >  3:195983/1‑149 (MQ=255)
     aaaGTCCCCATCCTTTTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCTCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGTAACAACAGGCCCTCCAGCGACCATCGTGTCAg                                                                                                                                             <  7:293168/148‑1 (MQ=255)
          ccccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                                                                                       >  6:220161/1‑149 (MQ=255)
           cccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                                                                                       <  7:30898/148‑1 (MQ=255)
            ccATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTc                                                                                                                                       >  2:44219/1‑147 (MQ=255)
              aTCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAg                                                                                                                                    >  6:128837/1‑148 (MQ=255)
                  ttGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAggg                                                                                                                               >  2:38818/1‑149 (MQ=255)
                     tgGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAACGGttt                                                                                                                            <  7:281025/149‑1 (MQ=255)
                         agcaACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGc                                                                                                                        <  2:108591/149‑1 (MQ=255)
                           caACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCATGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGccc                                                                                                                      <  3:295578/149‑1 (MQ=255)
                              cTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                                                                                                  <  2:285545/134‑1 (MQ=255)
                              cTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGaa                                                                                                                                  >  1:285545/1‑134 (MQ=255)
                                 tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGAt                                                                                                                >  5:207046/1‑149 (MQ=255)
                                 tCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGAt                                                                                                                >  5:202550/1‑149 (MQ=255)
                                      cTTAAAATCTAACAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCgg                                                                                                            <  7:270857/148‑1 (MQ=255)
                                       ttAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                                                                                          >  1:233341/1‑149 (MQ=255)
                                         aaaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGc                                                                                                          <  5:128837/147‑1 (MQ=255)
                                          aaaTCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCt                                                                                                        <  1:44219/148‑1 (MQ=255)
                                             tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                <  8:44835/105‑1 (MQ=255)
                                             tCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGAGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTc                                                                                                    >  4:120753/1‑149 (MQ=255)
                                              cTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTcc                                                                                                   >  7:284311/1‑149 (MQ=255)
                                                 accgCTGCCGCGCCCCCTGGGGGAGCGGGCGGGCCCGCTCCCACAGGGTCGGGGCAACTTTCAAGTCTGGCCACCAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAg                                                                                                <  8:175430/146‑1 (MQ=255)
                                                      tGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAg                                                                                           >  2:207424/1‑149 (MQ=255)
                                                          gcgcCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCg                                                                                       >  2:228923/1‑149 (MQ=255)
                                                                 cTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATcgc                                                                                <  5:40227/149‑1 (MQ=255)
                                                                     gggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa                                                                            >  1:132238/1‑149 (MQ=255)
                                                                      ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCaa                                                                            <  5:220161/148‑1 (MQ=255)
                                                                      ggAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc                                                                           <  1:228923/149‑1 (MQ=255)
                                                                      ggAGCGGGCACGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAAc                                                                           >  4:97459/1‑149 (MQ=255)
                                                                        aGCGGGCGTGCCCGCTCCCACAGAGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTc                                                                         <  3:120753/149‑1 (MQ=255)
                                                                                tGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                >  8:196170/1‑70 (MQ=255)
                                                                                tGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCgtgt                                                                                                                                                <  7:196170/70‑1 (MQ=255)
                                                                                  cccGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTc                                                               <  1:207424/149‑1 (MQ=255)
                                                                                   ccGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGGCCGACATCGCGCAACTCGTCATCGGTCa                                                              <  1:174914/149‑1 (MQ=255)
                                                                                         ccACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTg                                                          >  4:280584/1‑147 (MQ=255)
                                                                                         ccACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTg                                                          <  1:261780/147‑1 (MQ=255)
                                                                                           acaGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTgcag                                                       >  2:108920/1‑148 (MQ=255)
                                                                                           acaGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTgcag                                                       >  1:297403/1‑148 (MQ=255)
                                                                                                     tGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGc                                            <  8:284311/149‑1 (MQ=255)
                                                                                                       cAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCgg                                          <  6:207046/149‑1 (MQ=255)
                                                                                                       cAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCTTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCgg                                          <  6:202550/149‑1 (MQ=255)
                                                                                                          gTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTa                                       >  2:26193/1‑149 (MQ=255)
                                                                                                                 gTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGc                                >  6:83782/1‑149 (MQ=255)
                                                                                                                 gTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGc                                <  2:233341/149‑1 (MQ=255)
                                                                                                                       ggAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATgca                          <  5:83782/149‑1 (MQ=255)
                                                                                                                        gAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATgcag                         >  2:144348/1‑149 (MQ=255)
                                                                                                                              caGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATGCAGCATCAg                   >  5:128643/1‑149 (MQ=255)
                                                                                                                                               aTCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATGCAGCATCAGCCCCCAACGCCCCAAAc  <  1:144348/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACTTGAAAGTCCCCATCCTTGTGGCAGCAACTGTCTTGCTTAAAATCTAAAAGCTGACGCGCCCCCTGTGGGAGCGGGCGTGCCCGCTCCCACAGGGTCGGTGCAAGTTTCAAGTCTGGGGAACAACAGGCCCTCCAGCGACCATCGTGTCAGTCCTTCCAGAAGGGTTTCCGCCCTTCGATCCGGGCCTGCTCCCAGCTCAGCCCGACATCGCGCAACTCGTCATCGGTCAGCTGCAGCAACGCCCTGCGGGTATGCCAGCGATGCAGCATCAGCCCCCAACGCCCCAAAC  >  NC_002947/6023080‑6023371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑