Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,068,526 T→A 7.4% L390Q (CTG→CAG)  PP_5322 → metal ion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,068,5260TA 7.4% 155.7 / 2.5 54L390Q (CTG→CAG) PP_5322metal ion transporter
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base A (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GGCTACCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCG  >  NC_002947/6068378‑6068669
                                                                                                                                                    |                                                                                                                                               
ggCTACCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCctgct                                                                                                                                                 <  5:293073/149‑1 (MQ=255)
ggCTACCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCctgct                                                                                                                                                 >  4:279347/1‑149 (MQ=255)
  cTACCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTgg                                                                                                                                               <  3:171075/149‑1 (MQ=255)
     ccTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTa                                                                                                                                            >  8:77652/1‑149 (MQ=255)
         aCCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCg                                                                                                                                         <  3:279347/148‑1 (MQ=255)
          ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                       <  6:44848/149‑1 (MQ=255)
          ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                       <  4:59023/149‑1 (MQ=255)
                     gTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAt                                                                                                                            >  1:214058/1‑149 (MQ=255)
                      tGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGATGGGTATCGACCTTGACCACATc                                                                                                                           >  1:100774/1‑149 (MQ=255)
                          ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                               <  1:166762/141‑1 (MQ=38)
                          ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                               >  2:166762/1‑141 (MQ=38)
                          ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAgg                                                                                                                       <  8:70652/149‑1 (MQ=255)
                           gAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGc                                                                                                                      <  6:194150/149‑1 (MQ=255)
                               tGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGa                                                                                                                   >  1:11717/1‑148 (MQ=255)
                                  tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                   <  2:96067/129‑1 (MQ=255)
                                  tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                   >  1:96067/1‑129 (MQ=255)
                                       ggCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGc                                                                                                          >  7:238001/1‑149 (MQ=255)
                                          gACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCgg                                                                                                       <  2:214058/149‑1 (MQ=255)
                                               ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                >  7:121034/1‑117 (MQ=37)
                                               ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                <  8:121034/119‑3 (MQ=37)
                                                 aGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGCGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATc                                                                                                <  5:216688/149‑1 (MQ=255)
                                                    aCGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTAc                                                                                             <  7:77652/149‑1 (MQ=255)
                                                      gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                           >  5:20738/1‑149 (MQ=255)
                                                      gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCCGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                           <  5:264296/149‑1 (MQ=255)
                                                        gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                        >  7:111429/1‑102 (MQ=255)
                                                        gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                        <  8:111429/102‑1 (MQ=255)
                                                         cACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGAc                                                                                        >  6:306973/1‑149 (MQ=255)
                                                          aCCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACg                                                                                       <  2:11717/149‑1 (MQ=255)
                                                            cGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCt                                                                                     >  2:35196/1‑149 (MQ=255)
                                                               aaGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGTTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCaa                                                                                  <  2:100774/149‑1 (MQ=255)
                                                                    cGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGcgcg                                                                             >  4:32036/1‑149 (MQ=255)
                                                                        cGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGTGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGcc                                                                         <  7:280266/149‑1 (MQ=255)
                                                                          tGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                        >  6:107540/1‑148 (MQ=255)
                                                                            gCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaa                                                                     <  3:32036/149‑1 (MQ=255)
                                                                                    gCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAagaaga                                                                <  5:306973/146‑1 (MQ=255)
                                                                                     ccTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                            <  2:2265/149‑1 (MQ=255)
                                                                                      cTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAg                                                           >  2:172900/1‑149 (MQ=255)
                                                                                           aGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTg                                                      <  7:142529/149‑1 (MQ=255)
                                                                                            gCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTgg                                                     <  8:238001/149‑1 (MQ=255)
                                                                                                         cgggTGCGTGGCGAGACCCCACTGTTCAATCTCGAGCGCCTGCTTGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTAGAATGGCt                                        <  8:33310/147‑1 (MQ=255)
                                                                                                            gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                        >  2:2226/1‑114 (MQ=255)
                                                                                                            gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                        <  1:2226/114‑1 (MQ=255)
                                                                                                                gTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGGGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatc                                 <  1:141918/149‑1 (MQ=255)
                                                                                                                  ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                  >  2:135574/1‑114 (MQ=255)
                                                                                                                  ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                  <  1:135574/114‑1 (MQ=255)
                                                                                                                   gCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatcatc                              <  6:20738/149‑1 (MQ=255)
                                                                                                                        acCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCatcatcatc                           >  3:124846/1‑147 (MQ=255)
                                                                                                                          cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                        >  5:212888/1‑148 (MQ=255)
                                                                                                                          cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                        >  4:87651/1‑148 (MQ=255)
                                                                                                                          cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAACGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                        >  1:226294/1‑148 (MQ=255)
                                                                                                                                    aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                   >  4:180664/1‑111 (MQ=255)
                                                                                                                                    aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                   <  3:180664/111‑1 (MQ=255)
                                                                                                                                       gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                            <  1:72388/99‑1 (MQ=255)
                                                                                                                                       gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                            >  2:72388/1‑99 (MQ=255)
                                                                                                                                            gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGcccc         >  4:115781/1‑145 (MQ=255)
                                                                                                                                            gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGa     <  1:172900/149‑1 (MQ=255)
                                                                                                                                                ctgctgGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCg  >  1:33907/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCTACCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCG  >  NC_002947/6068378‑6068669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑