Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,068,529 G→C 7.4% G391A (GGT→GCT) ‡ PP_5322 → metal ion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,068,5290GC 7.4% 171.0 / 3.5 54G391A (GGT→GCT) ‡PP_5322metal ion transporter
Reads supporting (aligned to +/- strand):  ref base G (23/27);  new base C (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTGG  >  NC_002947/6068383‑6068675
                                                                                                                                                  |                                                                                                                                                  
ccTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTa                                                                                                                                                  >  8:77652/1‑149 (MQ=255)
    aCCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCg                                                                                                                                               <  3:279347/148‑1 (MQ=255)
     ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                             <  6:44848/149‑1 (MQ=255)
     ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                             <  4:59023/149‑1 (MQ=255)
                gTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAt                                                                                                                                  >  1:214058/1‑149 (MQ=255)
                 tGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGATGGGTATCGACCTTGACCACATc                                                                                                                                 >  1:100774/1‑149 (MQ=255)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                                     <  1:166762/141‑1 (MQ=38)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                                     >  2:166762/1‑141 (MQ=38)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAgg                                                                                                                             <  8:70652/149‑1 (MQ=255)
                      gAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGc                                                                                                                            <  6:194150/149‑1 (MQ=255)
                          tGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGa                                                                                                                         >  1:11717/1‑148 (MQ=255)
                             tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                         >  1:96067/1‑129 (MQ=255)
                             tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                         <  2:96067/129‑1 (MQ=255)
                                  ggCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGc                                                                                                                >  7:238001/1‑149 (MQ=255)
                                     gACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCgg                                                                                                             <  2:214058/149‑1 (MQ=255)
                                          ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                      >  7:121034/1‑117 (MQ=37)
                                          ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                      <  8:121034/119‑3 (MQ=37)
                                            aGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGCGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATc                                                                                                      <  5:216688/149‑1 (MQ=255)
                                               aCGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTAc                                                                                                   <  7:77652/149‑1 (MQ=255)
                                                 gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                                 >  5:20738/1‑149 (MQ=255)
                                                 gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCCGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                                 <  5:264296/149‑1 (MQ=255)
                                                   gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                              <  8:111429/102‑1 (MQ=255)
                                                   gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                              >  7:111429/1‑102 (MQ=255)
                                                    cACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGAc                                                                                              >  6:306973/1‑149 (MQ=255)
                                                     aCCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACg                                                                                             <  2:11717/149‑1 (MQ=255)
                                                       cGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCt                                                                                           >  2:35196/1‑149 (MQ=255)
                                                          aaGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGTTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCaa                                                                                        <  2:100774/149‑1 (MQ=255)
                                                               cGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGcgcg                                                                                   >  4:32036/1‑149 (MQ=255)
                                                                   cGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGTGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGcc                                                                               <  7:280266/149‑1 (MQ=255)
                                                                     tGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                              >  6:107540/1‑148 (MQ=255)
                                                                       gCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaa                                                                           <  3:32036/149‑1 (MQ=255)
                                                                               gCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAagaaga                                                                      <  5:306973/146‑1 (MQ=255)
                                                                                ccTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                  <  2:2265/149‑1 (MQ=255)
                                                                                 cTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAg                                                                 >  2:172900/1‑149 (MQ=255)
                                                                                      aGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTg                                                            <  7:142529/149‑1 (MQ=255)
                                                                                       gCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTgg                                                           <  8:238001/149‑1 (MQ=255)
                                                                                                    cgggTGCGTGGCGAGACCCCACTGTTCAATCTCGAGCGCCTGCTTGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTAGAATGGCt                                              <  8:33310/147‑1 (MQ=255)
                                                                                                       gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                              >  2:2226/1‑114 (MQ=255)
                                                                                                       gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                              <  1:2226/114‑1 (MQ=255)
                                                                                                           gTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGGGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatc                                       <  1:141918/149‑1 (MQ=255)
                                                                                                             ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                        <  1:135574/114‑1 (MQ=255)
                                                                                                             ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                        >  2:135574/1‑114 (MQ=255)
                                                                                                              gCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatcatc                                    <  6:20738/149‑1 (MQ=255)
                                                                                                                   acCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCatcatcatc                                 >  3:124846/1‑147 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                              >  5:212888/1‑148 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                              >  4:87651/1‑148 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAACGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                              >  1:226294/1‑148 (MQ=255)
                                                                                                                               aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                         >  4:180664/1‑111 (MQ=255)
                                                                                                                               aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                         <  3:180664/111‑1 (MQ=255)
                                                                                                                                  gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                  >  2:72388/1‑99 (MQ=255)
                                                                                                                                  gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                  <  1:72388/99‑1 (MQ=255)
                                                                                                                                       gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGcccc               >  4:115781/1‑145 (MQ=255)
                                                                                                                                       gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGa           <  1:172900/149‑1 (MQ=255)
                                                                                                                                           ctgctgGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCg        >  1:33907/1‑148 (MQ=255)
                                                                                                                                                gggTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTgg  >  3:240375/1‑149 (MQ=255)
                                                                                                                                                 ggTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTgg  <  1:35196/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTGG  >  NC_002947/6068383‑6068675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑