Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,068,530 T→G 7.2% G391G (GGT→GGG) ‡ PP_5322 → metal ion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,068,5300TG 7.2% 170.1 / 3.7 56G391G (GGT→GGG) ‡PP_5322metal ion transporter
Reads supporting (aligned to +/- strand):  ref base T (24/28);  new base G (2/2);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTGGCC  >  NC_002947/6068383‑6068677
                                                                                                                                                   |                                                                                                                                                   
ccTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTa                                                                                                                                                    >  8:77652/1‑149 (MQ=255)
    aCCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCg                                                                                                                                                 <  3:279347/148‑1 (MQ=255)
     ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                               <  6:44848/149‑1 (MQ=255)
     ccATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGAc                                                                                                                                               <  4:59023/149‑1 (MQ=255)
                gTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAt                                                                                                                                    >  1:214058/1‑149 (MQ=255)
                 tGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGATGGGTATCGACCTTGACCACATc                                                                                                                                   >  1:100774/1‑149 (MQ=255)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                                       <  1:166762/141‑1 (MQ=38)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGAAca                                                                                                                                       >  2:166762/1‑141 (MQ=38)
                     ggAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAgg                                                                                                                               <  8:70652/149‑1 (MQ=255)
                      gAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGc                                                                                                                              <  6:194150/149‑1 (MQ=255)
                          tGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGa                                                                                                                           >  1:11717/1‑148 (MQ=255)
                             tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                           >  1:96067/1‑129 (MQ=255)
                             tGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTg                                                                                                                                           <  2:96067/129‑1 (MQ=255)
                                  ggCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGc                                                                                                                  >  7:238001/1‑149 (MQ=255)
                                     gACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCgg                                                                                                               <  2:214058/149‑1 (MQ=255)
                                          ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                        >  7:121034/1‑117 (MQ=37)
                                          ccAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGATACCCAGCAGGCGCTCGACCTTGaac                                                                                                                                        <  8:121034/119‑3 (MQ=37)
                                            aGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGCGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATc                                                                                                        <  5:216688/149‑1 (MQ=255)
                                               aCGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTAc                                                                                                     <  7:77652/149‑1 (MQ=255)
                                                 gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                                   >  5:20738/1‑149 (MQ=255)
                                                 gAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCCGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACga                                                                                                   <  5:264296/149‑1 (MQ=255)
                                                   gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                                <  8:111429/102‑1 (MQ=255)
                                                   gCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGa                                                                                                                                                >  7:111429/1‑102 (MQ=255)
                                                    cACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGAc                                                                                                >  6:306973/1‑149 (MQ=255)
                                                     aCCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACg                                                                                               <  2:11717/149‑1 (MQ=255)
                                                       cGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCt                                                                                             >  2:35196/1‑149 (MQ=255)
                                                          aaGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGTTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCaa                                                                                          <  2:100774/149‑1 (MQ=255)
                                                               cGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGcgcg                                                                                     >  4:32036/1‑149 (MQ=255)
                                                                   cGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGTGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGcc                                                                                 <  7:280266/149‑1 (MQ=255)
                                                                     tGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                                >  6:107540/1‑148 (MQ=255)
                                                                       gCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaa                                                                             <  3:32036/149‑1 (MQ=255)
                                                                               gCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAagaaga                                                                        <  5:306973/146‑1 (MQ=255)
                                                                                ccTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                    <  2:2265/149‑1 (MQ=255)
                                                                                 cTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAg                                                                   >  2:172900/1‑149 (MQ=255)
                                                                                      aGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTg                                                              <  7:142529/149‑1 (MQ=255)
                                                                                       gCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTgg                                                             <  8:238001/149‑1 (MQ=255)
                                                                                                    cgggTGCGTGGCGAGACCCCACTGTTCAATCTCGAGCGCCTGCTTGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTAGAATGGCt                                                <  8:33310/147‑1 (MQ=255)
                                                                                                       gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                                <  1:2226/114‑1 (MQ=255)
                                                                                                       gTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCt                                                                                >  2:2226/1‑114 (MQ=255)
                                                                                                           gTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGGGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatc                                         <  1:141918/149‑1 (MQ=255)
                                                                                                             ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                          <  1:135574/114‑1 (MQ=255)
                                                                                                             ggCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTgaagaa                                                                          >  2:135574/1‑114 (MQ=255)
                                                                                                              gCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGcatcatc                                      <  6:20738/149‑1 (MQ=255)
                                                                                                                   acCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCatcatcatc                                   >  3:124846/1‑147 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                                >  5:212888/1‑148 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                                >  4:87651/1‑148 (MQ=255)
                                                                                                                     cccACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACAACGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCaag                                >  1:226294/1‑148 (MQ=255)
                                                                                                                               aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                           >  4:180664/1‑111 (MQ=255)
                                                                                                                               aaGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAg                                                           <  3:180664/111‑1 (MQ=255)
                                                                                                                                  gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                    >  2:72388/1‑99 (MQ=255)
                                                                                                                                  gTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGaa                                                                    <  1:72388/99‑1 (MQ=255)
                                                                                                                                       gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGcccc                 >  4:115781/1‑145 (MQ=255)
                                                                                                                                       gcgcCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGa             <  1:172900/149‑1 (MQ=255)
                                                                                                                                           ctgctgGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCg          >  1:33907/1‑148 (MQ=255)
                                                                                                                                                gggTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTgg    >  3:240375/1‑149 (MQ=255)
                                                                                                                                                 ggTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTgg    <  1:35196/148‑1 (MQ=255)
                                                                                                                                                   tATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTGGcc  >  5:201406/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGACCATGGAAGACGTGCTGGAAGTGCTGGTAGGCGACATCCAGGACGAGCACCGCAAGACCGAGCGTGGCATTCTTGCCTACCAGCCGGGCAAGCTGCTGGTGCGTGGCGACACCCCACTGTTCAAGGTCGAGCGCCTGCTGGGTATCGACCTTGACCACATCGAGGCCGAAACCCTGGCCGGGTTGATCTACGAGACGCTCAAGCGCGTGCCTGAAGAAGAGGAAGTGCTGGAGGTCGAAGGGCTGCGCATCATCATCAAGAAGATGAAAGGCCCCAAGATCGTACTGGCC  >  NC_002947/6068383‑6068677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑