Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,101,827 T→A 7.8% Y327N (TAC→AAC)  uvrD → DNA‑dependent ATPase I/helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,101,8270TA 7.8% 150.6 / 2.1 51Y327N (TAC→AAC) uvrDDNA‑dependent ATPase I/helicase II
Reads supporting (aligned to +/- strand):  ref base T (24/23);  new base A (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAAATGATCCGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCC  >  NC_002947/6101680‑6101974
                                                                                                                                                   |                                                                                                                                                    
gAAATGATCCGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTa                                                                                                                                                     >  1:240759/1‑149 (MQ=255)
    tGATCCGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAAc                                                                                                                                                 <  6:178590/149‑1 (MQ=255)
        ccGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAg                                                                                                                                              >  6:212412/1‑148 (MQ=255)
         cGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCa                                                                                                                                            <  3:254504/149‑1 (MQ=255)
              ggAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCacgac                                                                                                                                        >  4:292081/1‑148 (MQ=255)
               gAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCacgac                                                                                                                                        <  1:230352/147‑1 (MQ=255)
                 gCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAg                                                                                                                                    >  6:141935/1‑149 (MQ=255)
                     aaCTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCAACAACGAGCACGACGAAgcgcg                                                                                                                                >  3:67218/1‑149 (MQ=255)
                     aaCTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCAACAACGAGCACGACGAAgcgcg                                                                                                                                >  3:63128/1‑149 (MQ=255)
                      aCTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAgcgcg                                                                                                                                <  5:170304/148‑1 (MQ=255)
                          ccGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTa                                                                                                                             <  5:212412/147‑1 (MQ=255)
                           cGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGaa                                                                                                                                     >  4:288643/1‑138 (MQ=255)
                           cGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGaa                                                                                                                                     <  3:288643/138‑1 (MQ=255)
                           cGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑Gt                                                                                                                         >  2:261978/1‑149 (MQ=255)
                            gCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTc                                                                                                                        <  8:72678/149‑1 (MQ=255)
                            gCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTc                                                                                                                        <  5:141935/149‑1 (MQ=255)
                             cTCCACCTGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑g                                                                                                                          <  3:66386/146‑1 (MQ=255)
                                cACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGt                                                                                                                      <  2:240759/147‑1 (MQ=255)
                                cACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTgg                                                                                                                    <  3:292081/149‑1 (MQ=255)
                                cACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACATAGCGCGCTTC‑GTCGTgc                                                                                                                    <  7:246461/149‑2 (MQ=255)
                                     gcggcATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAAc                                                                                                                >  8:18681/1‑148 (MQ=255)
                                         cATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCAACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCg                                                                                                           <  4:67218/149‑1 (MQ=255)
                                         cATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCAACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCg                                                                                                           <  4:63128/149‑1 (MQ=255)
                                             cTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCgaga                                                                                                        <  7:18681/148‑1 (MQ=255)
                                                aaGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGc                                                                                                      <  2:282017/147‑1 (MQ=255)
                                                       ccAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGGTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAg                                                                                             >  6:80603/1‑149 (MQ=255)
                                                           tgcgcTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAgg                                                                                         <  1:180396/148‑1 (MQ=255)
                                                           cgcgCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTATGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAgg                                                                                         <  2:97225/149‑1 (MQ=255)
                                                                     gCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATgcgcg                                                                               >  7:12365/1‑149 (MQ=255)
                                                                     gCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATgcgc                                                                                <  1:261978/148‑1 (MQ=255)
                                                                        aacaacAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCaa                                                                            <  8:12365/149‑1 (MQ=255)
                                                                                     gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATc                                                               >  8:204879/1‑149 (MQ=255)
                                                                                          ggCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTa                                                          >  8:279312/1‑149 (MQ=255)
                                                                                          ggCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCAATGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATCATCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTg                                                            <  1:91805/148‑1 (MQ=255)
                                                                                            cAAGGAACTGTGGACCGACATGGTAGAAGGAGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGAACGCCATCCTGTa                                                          >  5:221374/1‑147 (MQ=255)
                                                                                             aaGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCg                                                       >  7:218821/1‑149 (MQ=255)
                                                                                                 aaCTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTcc                                                   <  8:218821/149‑1 (MQ=255)
                                                                                                                 gtgcgaaggagaaCCACTGACCCTGTACGCGGCCTACAACGAGCAAGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCAGGTCAAGCAGGGCAATGCGCGCAACCAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgcgcg                                   >  4:27003/3‑149 (MQ=255)
                                                                                                                 gggCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgcgcg                                   >  2:41382/1‑149 (MQ=255)
                                                                                                                  ggcgaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATc                                                               >  3:289435/1‑120 (MQ=255)
                                                                                                                  ggcgaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATc                                                               <  4:289435/120‑1 (MQ=255)
                                                                                                                     gaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCt                               >  3:275638/1‑149 (MQ=255)
                                                                                                                      aaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTg                              >  2:110863/1‑149 (MQ=255)
                                                                                                                             aCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAgg                       >  3:143545/1‑149 (MQ=255)
                                                                                                                             aCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAgg                       <  6:99759/149‑1 (MQ=255)
                                                                                                                               cACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGcc                     <  4:143545/149‑1 (MQ=255)
                                                                                                                                    aCCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCctgct                >  2:228191/1‑149 (MQ=255)
                                                                                                                                      ccTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCtgctgc              >  1:58791/1‑149 (MQ=255)
                                                                                                                                          tACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTgcgc            >  6:164866/1‑147 (MQ=255)
                                                                                                                                                 ccTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATc   >  7:115067/1‑149 (MQ=255)
                                                                                                                                                  cTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATcc  >  6:298228/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GAAATGATCCGCCTGGAGCAGAACTACCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTAT‑GTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCC  >  NC_002947/6101680‑6101974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑