Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,148,487 A→G 10.2% intergenic (‑671/‑2503) PP_5393 ← / → PP_5395 metal‑binding chaperone/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,148,4870AG10.2% 102.1 / 2.3 39intergenic (‑671/‑2503)PP_5393/PP_5395metal‑binding chaperone/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (18/17);  new base G (2/2);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CACCGTGGCCACGGATAGTGTTCGGGCCCGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGCCGTAGT  >  NC_002947/6148346‑6148627
                                                                                                                                              |                                                                                                                                            
cACCGTGGCCACGGATAGTGTTCGGGCCCGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACTTCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGt                                                                                                                                          <  3:223098/146‑1 (MQ=255)
 aCCGTGGCCACGGATAGTGTTCGGGCCCGCAGGATACACATCAGGT‑CGCCCTTGTCCATCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATGAGCCCTGGCCAGCGCGGGGGCATCGTTGa                                                                                                                                       <  2:116217/148‑1 (MQ=255)
  ccGTGGCCACGGATAGGGTTCGGGCCCGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATc                                                                                                                                     <  7:139668/149‑1 (MQ=255)
             gATAGTGTTCGGGCCCGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGc                                                                                                                          >  2:215740/1‑149 (MQ=255)
                    ttCGGGCCCGCAGGATACCCATCAGGTGCGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATgg                                                                                                                     >  1:170617/1‑148 (MQ=255)
                          cccGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCgg                                                                                                             <  7:265126/149‑1 (MQ=255)
                                   tACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCg                                                                                                    <  2:93742/149‑1 (MQ=255)
                                      ccATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGa                                                                                                                                       >  7:198626/1‑111 (MQ=255)
                                      ccATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGa                                                                                                                                       <  8:198626/111‑1 (MQ=255)
                                           aGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAg                                                                                            <  2:170617/149‑1 (MQ=255)
                                             gT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCGTCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAg                                                                                            >  1:228481/1‑147 (MQ=255)
                                                cGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAgc                                                                                        >  6:135400/1‑149 (MQ=255)
                                                          cAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCaa                                                                                                                  >  6:244248/1‑113 (MQ=255)
                                                          cAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCaa                                                                                                                  <  5:244248/113‑1 (MQ=255)
                                                           aGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTg                                                                             <  2:182890/149‑1 (MQ=255)
                                                                       gcgcTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGTCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTc                                                                 >  5:235663/1‑149 (MQ=255)
                                                                             ttCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGtt                                                            >  5:208083/1‑148 (MQ=255)
                                                                                aTGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTAACGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCa                                                         <  1:215740/148‑1 (MQ=255)
                                                                                          cGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTcc                                                                         >  6:310894/1‑122 (MQ=255)
                                                                                          cGGTGCCGGTTCCCAAGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTg                                               >  8:92900/1‑148 (MQ=255)
                                                                                           ggTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTgg                                              <  5:135400/148‑1 (MQ=255)
                                                                                                 ggTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCGTCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCt                                       <  2:228481/149‑1 (MQ=255)
                                                                                                  gTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCGTCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCt                                       >  5:75478/1‑148 (MQ=255)
                                                                                                  gTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCGTCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTc                                      <  6:75478/149‑1 (MQ=255)
                                                                                                      ccATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGt                                                             <  5:23249/122‑1 (MQ=255)
                                                                                                      ccATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGt                                                             >  6:23249/1‑122 (MQ=255)
                                                                                                      ccATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATg                                  >  7:91037/1‑149 (MQ=255)
                                                                                                           gCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAg                             >  3:255625/1‑149 (MQ=255)
                                                                                                                  cGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTc                      <  6:290468/149‑1 (MQ=255)
                                                                                                                   gACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTcg                     >  3:24154/1‑149 (MQ=255)
                                                                                                                      aTCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGa                  >  2:135432/1‑149 (MQ=255)
                                                                                                                        cAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGAcc                >  8:59313/1‑149 (MQ=255)
                                                                                                                           cccGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAgtt                           >  7:276747/1‑135 (MQ=255)
                                                                                                                           cccGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAgtt                           <  8:276747/135‑1 (MQ=255)
                                                                                                                           cccGGGCCAGCGCGGGGGCATCGGTGCTCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGGGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGTCTTCCTCAATGCCGAGGTGTTTCGCGCCCGCa             >  8:63557/1‑149 (MQ=255)
                                                                                                                                 ccAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGc        >  2:5340/1‑148 (MQ=255)
                                                                                                                                 ccAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGc        <  1:5340/148‑1 (MQ=255)
                                                                                                                                    gcgcGGGGGCATCGTTGATCCCATCTCCTGTCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGCCGTa    <  6:235663/149‑1 (MQ=255)
                                                                                                                                      gcgGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGCCGTAGt  <  6:208083/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
CACCGTGGCCACGGATAGTGTTCGGGCCCGCAGGATACCCATCAGGT‑CGCCCTTGACCAGCGTCAACTGGGCGCTGTTCATGGCAACATCGGTGCCGGTTCCCATGGCGATCCCGACATCAGCCCGGGCCAGCGCGGGGGCATCGTTGATCCCATCTCCTGCCATGGCAACCCGGCGCTTATCGCCCTGCAGGAGCGCCACCAGTTGCTCCTTGTCCTCGGGTTTCACTTCTCCGTGGACTTCCTCAATGCCGAGTTGTTTCGCGACCGCACGTGCCGTAGT  >  NC_002947/6148346‑6148627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑