Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,166,470 G→T 9.1% intergenic (‑229/‑451) PP_5406 ← / → PP_5407 transposase/TnsD‑like transposition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,166,4700GT 9.1% 117.6 / 2.2 43intergenic (‑229/‑451)PP_5406/PP_5407transposase/TnsD‑like transposition protein
Reads supporting (aligned to +/- strand):  ref base G (17/22);  new base T (2/2);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01

CTGCGGGCAGGTAACCGCGTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCTTCGCTGACG  >  NC_002947/6166322‑6166618
                                                                                                                                                    |                                                                                                                                                    
cTGCGGGCAGGTAACCGCGTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAgg                                                                                                                                                      <  1:54858/149‑1 (MQ=255)
   cGGGCAGGTAACCGCGTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTc                                                                                                                                                    <  8:205901/148‑1 (MQ=255)
          gTAACCGCGTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTgg                                                                                                                                            <  2:68225/149‑1 (MQ=255)
                  gTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGTGCGACCTTGGGCCCTTGt                                                                                                                                    <  8:155116/149‑1 (MQ=255)
                            gtgGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGAtt                                                                                                                          >  2:250240/1‑149 (MQ=255)
                            gtgGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGAt                                                                                                                           <  2:213083/148‑1 (MQ=255)
                             tgGAAAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTCACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTAGGGCCTTTGTCTATCTGAtt                                                                                                                          >  6:181393/1‑148 (MQ=255)
                                        gagaTTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCtctc                                                                                                              >  5:298034/1‑149 (MQ=255)
                                             tCTTGTGGTTGAGCGAATCCGTGCTAAACTTGAAGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAAGGCCCAAGTGCGACCTTGGGCCCTTGt                                                                                                                                    <  8:175390/122‑1 (MQ=37)
                                             tCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGAAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAAGGCCCAAGTGAGACCTTGGGCCCTTGt                                                                                                                                    >  7:175390/1‑122 (MQ=255)
                                              cTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTTAGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTta                                                                                                         <  1:250240/148‑1 (MQ=255)
                                              cTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTta                                                                                                         <  7:48772/148‑1 (MQ=255)
                                                    gATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTagctagct                                                                                                  <  1:44369/149‑1 (MQ=255)
                                                              tCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTgtcgt                                                                                             >  6:250138/1‑144 (MQ=255)
                                                              tCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTgtcgt                                                                                             <  5:250138/144‑1 (MQ=255)
                                                                  tGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttc                                                                                    >  1:16751/1‑149 (MQ=255)
                                                                    cGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttct                                                                                   <  2:16751/148‑1 (MQ=255)
                                                                    cGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCACACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttctt                                                                                  >  2:58834/1‑149 (MQ=255)
                                                                     gAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttcttt                                                                                 <  6:298034/149‑1 (MQ=255)
                                                                      aaaCTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttctttc                                                                                <  6:121064/149‑1 (MQ=255)
                                                                             aCGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAgg                                                                          <  4:2179/148‑1 (MQ=255)
                                                                                tttCACATTCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTa                                                                      <  5:159643/149‑1 (MQ=255)
                                                                                         cAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAttt                                                                                     >  2:283820/1‑125 (MQ=255)
                                                                                         cAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAttt                                                                                     <  1:283820/125‑1 (MQ=255)
                                                                                             ggATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGtctc                                                         <  2:5780/149‑1 (MQ=255)
                                                                                              gATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTcc                                                        >  1:278342/1‑149 (MQ=255)
                                                                                                     aaTGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGAtt                                                 >  3:123813/1‑149 (MQ=255)
                                                                                                                   gTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCAc                                   >  8:166603/1‑149 (MQ=255)
                                                                                                                   gTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCAc                                   >  1:166932/1‑149 (MQ=255)
                                                                                                                    tGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACg                                  <  4:123813/149‑1 (MQ=255)
                                                                                                                                  gatagacaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGcc                                      >  6:111993/6‑131 (MQ=255)
                                                                                                                                  gatagacaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGcc                                      <  5:111993/126‑1 (MQ=255)
                                                                                                                                      cacaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTtctct                 >  7:1861/1‑148 (MQ=255)
                                                                                                                                      cacaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTCAATATCTTTCGTTAGGTCTTCCTTAGGCGTCTCCGCCGATTGTGATCTTGACCACGCTCACGAGCCCTtctct                 >  7:78784/1‑148 (MQ=255)
                                                                                                                                      cacaAGGCCCCAAGGTCGCACTTGGGCCTTTGTATATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTtctctc                <  4:72761/149‑1 (MQ=255)
                                                                                                                                       acaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttctttc                                                                                >  8:176818/1‑84 (MQ=255)
                                                                                                                                       acaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAAtttctttc                                                                                <  7:176818/84‑1 (MQ=255)
                                                                                                                                       acaAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCa               <  2:278342/149‑1 (MQ=255)
                                                                                                                                        caAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAg              >  1:285727/1‑149 (MQ=255)
                                                                                                                                        caAAGGCCCAAGTGCGACCTTGGGCCTTTGTATATCTGATTTCAAGAGCTATCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACATTAGGCGTCTCCGCCGATTGTGATATTGCCCACGCTCACGAGCCCTTCTCTCAg              >  3:231737/1‑149 (MQ=255)
                                                                                                                                           gggCCCAAGGTCGCACTGGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTGTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCt           <  5:181393/149‑1 (MQ=255)
                                                                                                                                                 aaGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCTTCGCTg     <  8:1861/149‑1 (MQ=255)
                                                                                                                                                  aGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTtctctc                >  2:118753/1‑137 (MQ=255)
                                                                                                                                                  aGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTtctctc                <  1:118753/137‑1 (MQ=255)
                                                                                                                                                   ggTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCTTCGCTGAc   >  7:52686/1‑149 (MQ=255)
                                                                                                                                                    gTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCTTCGCTGACg  >  6:43731/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGCGGGCAGGTAACCGCGTTTCCGGTTGTGGATAACGCGGAGATTCTTGTGGATGAGCGAATCCGTGCGAAACTTGACGTTTCACATGCAAGGGATAGACAATGGAGGAGTCAGGTGCCTTTTCTGACTTATCCACAAGGGCCCAAGGTCGCACTTGGGCCTTTGTCTATCTGATTTCCAGCGCTCTCGTTTAGCTAGCTGTCGTCTGAATTTCTTTCGTTAGGTCTACCTTAGGCGTCTCCGCCGATTGTGATCTTGCCCACGCTCACGAGCCCTTCTCTCAGGCTTCGCTGACG  >  NC_002947/6166322‑6166618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑