Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 519,619 C→A 11.7% G336G (GGC→GGA)  argC → N‑acetyl‑gamma‑glutamyl‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947519,6190CA11.7% 166.0 / 12.1 60G336G (GGC→GGA) argCN‑acetyl‑gamma‑glutamyl‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (30/23);  new base A (5/2);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

AACGTCTGCCGCATTGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATCGAGTTT  >  NC_002947/519476‑519763
                                                                                                                                               |                                                                                                                                                
aaCGTCTGCCGCATTGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTc                                                                                                                                             >  1:228145/1‑149 (MQ=255)
        ccGCATTGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCg                                                                                                                                     >  6:9175/1‑149 (MQ=255)
         cggaTTGCCGTTCATCGTCCGGAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCgg                                                                                                                                    <  2:81594/146‑1 (MQ=255)
            aTTGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCtt                                                                                                                                 <  1:57274/149‑1 (MQ=255)
             ttGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACACTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTtg                                                                                                                                >  2:47922/1‑149 (MQ=255)
                  gTTCATCGTCCGCAGTGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACGTGGTCAAGGGTGCCTCCGGGCTGGCGGTGCAGAACCTCAAGATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTGCCATGCCGGCTTGCTGcc                                                                                                                           <  4:82794/149‑1 (MQ=255)
                     cATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGt                                                                                                                         >  7:215962/1‑148 (MQ=255)
                       tCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGcgc                                                                                                                                     >  5:80238/1‑132 (MQ=255)
                       tCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGcgc                                                                                                                                     <  6:80238/134‑3 (MQ=255)
                       tCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAg                                                                                                                      <  4:67467/149‑1 (MQ=255)
                          tCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGgcc                                                                                                                   >  7:245470/1‑149 (MQ=255)
                            cGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCGTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGgccgc                                                                                                                 >  2:76368/1‑149 (MQ=255)
                            cGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGgccgc                                                                                                                 >  2:268750/1‑149 (MQ=255)
                                 ggtggtGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCa                                                                                                            >  8:268015/1‑149 (MQ=255)
                                   tggtgATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACACTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCAtt                                                                                                          <  1:47922/149‑1 (MQ=255)
                                        aTCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGaa                                                                                                      <  5:9175/148‑1 (MQ=255)
                                             gtggtggtgCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAgg                                                                                                  <  8:15309/147‑1 (MQ=255)
                                             gtggtggtgCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAg                                                                                                   <  2:228145/146‑1 (MQ=255)
                                                      cTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGACTGGATGAGCGCATGGGCCTGTCACATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGaaaacg                                                                                                  >  2:259676/1‑136 (MQ=255)
                                                       tttCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGc                                                                                      >  4:246342/1‑149 (MQ=255)
                                                       tttCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGGAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGc                                                                                      >  4:83805/1‑149 (MQ=255)
                                                       tttCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCACAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGc                                                                                      >  6:140962/1‑149 (MQ=255)
                                                         tCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCg                                                                                     <  8:245470/148‑1 (MQ=255)
                                                          cGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCgg                                                                                    <  8:253976/148‑1 (MQ=255)
                                                          cGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCgg                                                                                    >  7:253976/1‑148 (MQ=255)
                                                               aTCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCttt                                                                              <  8:215962/149‑1 (MQ=255)
                                                               aTCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCttt                                                                              >  8:52724/1‑149 (MQ=255)
                                                               aTCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCttt                                                                              >  3:276110/1‑149 (MQ=255)
                                                               aTCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCtt                                                                               <  7:268015/148‑1 (MQ=255)
                                                                tCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCtttt                                                                             >  2:100493/1‑149 (MQ=255)
                                                                   aCAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTg                                                                           >  6:245674/1‑148 (MQ=255)
                                                                    cAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTg                                                                           >  2:143550/1‑147 (MQ=255)
                                                                    cAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGCCCCGCACGACGCGGGCCttttccaa                                                                         <  8:260282/149‑5 (MQ=255)
                                                                         tGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTcg                                                                    >  6:58000/1‑149 (MQ=255)
                                                                            tCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGa                                                                 <  7:52724/149‑1 (MQ=255)
                                                                                 ggTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTa                                                              >  8:251678/1‑147 (MQ=255)
                                                                                            ccAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGcgtc                                                                                          >  4:42680/1‑108 (MQ=255)
                                                                                            ccAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGcgtc                                                                                          <  3:42680/108‑1 (MQ=255)
                                                                                            ccAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAAt                                                  >  3:67240/1‑148 (MQ=255)
                                                                                             cAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTgg                                                                                                        >  8:169268/1‑93 (MQ=255)
                                                                                             cAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTgg                                                                                                        <  7:169268/93‑1 (MQ=255)
                                                                                                gCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCtt                                              >  2:265280/1‑148 (MQ=255)
                                                                                                 cGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGa                                            <  3:246342/149‑1 (MQ=255)
                                                                                                         taCCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGAttt                                     >  6:27309/2‑148 (MQ=255)
                                                                                                            cTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTtctc                                 >  4:232440/1‑149 (MQ=255)
                                                                                                             tCAACATTCTGTTCGGTCTGGATGAGCGCAAGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCg                                >  8:186693/1‑149 (MQ=255)
                                                                                                                 cATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGa                             >  2:272784/1‑148 (MQ=255)
                                                                                                                     cTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGc                         <  1:143550/148‑1 (MQ=255)
                                                                                                                        ttCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGa                      >  5:300867/1‑148 (MQ=255)
                                                                                                                        ttCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGa                      <  5:140962/148‑1 (MQ=255)
                                                                                                                             tCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATgc                <  6:300867/149‑1 (MQ=255)
                                                                                                                                  aTGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCTCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGt            <  4:276110/148‑1 (MQ=255)
                                                                                                                                     ccggcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATc        >  1:136886/4‑149 (MQ=255)
                                                                                                                                      cggcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATc        >  2:15475/3‑148 (MQ=255)
                                                                                                                                      cggcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATc        >  2:20123/3‑148 (MQ=255)
                                                                                                                                       ggcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATc        >  4:295414/2‑147 (MQ=255)
                                                                                                                                        gcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGAt                     <  6:109006/133‑1 (MQ=255)
                                                                                                                                        gcATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGAt                     >  5:109006/1‑133 (MQ=255)
                                                                                                                                         cATGGGACAGGCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATCGAGt    <  2:136886/149‑1 (MQ=255)
                                                                                                                                           tGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATCGAGttt  <  1:265280/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AACGTCTGCCGCATTGCCGTTCATCGTCCGCAGGGTGGTGATCTGGTGGTGGTGCTTTCGGTGATCGACAACCTGGTCAAGGGTGCCTCCGGCCAGGCGGTGCAGAACCTCAACATTCTGTTCGGTCTGGATGAGCGCATGGGCCTGTCCCATGCCGGCTTGCTGCCGTAAGGCCGCCAGCATTGGAAAAGGCCCGCGTCGTGCGGGCCTTTTTGCAGGTCGCGACTAAAGCCGCACAATTCTTGACCGATTTTCTCGGGAAAGCGGATAATGCGCGTCATCGAGTTT  >  NC_002947/519476‑519763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑