| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 524,606 | G→A | 9.3% | intergenic (+473/‑343) | tyrS → / → PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 524,606 | 0 | G | A | 9.3% | 105.0 / 2.9 | 44 | intergenic (+473/‑343) | tyrS/PP_16SC | tyrosine‑‑tRNA ligase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (17/22); new base A (2/2); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01 | |||||||||||
CCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTG > NC_002947/524484‑524749 | ccccACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTc < 8:150209/149‑1 (MQ=32) cACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCg > 5:298111/1‑149 (MQ=32) cAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTGTGATTCGCCTCCCGCTACga < 4:279558/149‑1 (MQ=17) cAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACga < 4:51975/149‑1 (MQ=32) atctaTCGGAAGGAAAAACTCCTTGAGATTCTATGTGTTAAGTAGTTAAGGCAATAGCTGAAAGGTCTTCGATCGAATGATCGAATGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACgagag < 7:199322/145‑1 (MQ=11) aCATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGAt < 5:12407/149‑1 (MQ=32) aTCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCg < 1:69164/149‑1 (MQ=32) tCGGAACGAAAAACTCCTAGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGCTTGAGATGATGGATGACAGCCATTCCAAGTGCTGTATGATTAGCCTCCAGCTACgagac > 8:199322/1‑143 (MQ=11) gAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCa > 8:252740/1‑147 (MQ=32) gAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGc > 2:94865/1‑149 (MQ=32) gagaTTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGATACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTg > 5:23601/1‑147 (MQ=17) agaTTCAATGAGTTAAGTAGTTAATGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGaa < 3:59493/148‑1 (MQ=17) gaTTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCaa < 6:4050/138‑1 (MQ=32) gaTTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCaa > 5:4050/1‑138 (MQ=32) aaTGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCACTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAAc > 1:89352/1‑149 (MQ=17) aGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAg < 2:261732/148‑1 (MQ=32) aaGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTtct > 7:22525/1‑149 (MQ=32) gtTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCACTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCa < 2:89352/148‑1 (MQ=17) ttAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAg > 8:22543/1‑148 (MQ=32) acgCAAAAGCTGAACGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACTAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGaaaa < 6:105659/147‑1 (MQ=12) aaaGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCa < 6:298111/149‑1 (MQ=34) gCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCtt < 4:180856/149‑1 (MQ=34) tCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCACGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCTCAGAAAACTTCAAAATAAACGCTTGACAGGc < 7:252740/149‑1 (MQ=21) aaTGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCt < 4:50960/149‑1 (MQ=37) aTCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAg < 7:162097/148‑1 (MQ=37) tCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAgg > 2:36818/1‑148 (MQ=37) aaaGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAg > 2:48273/1‑149 (MQ=35) gCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGt < 4:165574/149‑1 (MQ=34) gTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGa < 7:22543/149‑1 (MQ=34) gTTGAGATGATGGTTGACAGAGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGAGAGCCAAGTGTTTGAAGCTAAACGAGTTTATCGCAGAAAACTTAAAAATAAACTCTTGACAGGCCCTGAGTAAAGAGTAg > 8:226710/1‑148 (MQ=11) agaTGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATgc > 5:66658/1‑149 (MQ=34) tgatgGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATGGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATgcgc < 5:257984/148‑1 (MQ=21) gatgGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTa < 1:65797/139‑1 (MQ=34) gatgGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTa > 2:65797/1‑139 (MQ=34) atcataCAGCACTTGGAAGCGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTc > 5:4296/6‑149 (MQ=11) ataCAGCACTTGGAAGCGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCgg < 6:4296/146‑1 (MQ=255) gACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 7:41009/1‑149 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGaa > 6:162437/1‑134 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGaa < 5:162437/134‑1 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 6:218894/1‑148 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 7:79841/1‑148 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt < 5:218894/148‑1 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTg < 6:66658/149‑1 (MQ=34) aCAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAAAGCTTGACAGGCCCTGAGGAAAGCGGAGAATGCGCGCCTCGGtt > 3:50277/1‑148 (MQ=21) | CCCCACTTCCAGCGACATCGGAACGAAAAACTCCTTGAGATTCAATGAGTTAAGTAGTTAAGGCAAAAGCTGAAAGGGCTACGATCGAATGATCGAAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAGAAAACTTCAAAATAAACGCTTGACAGGCCCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTG > NC_002947/524484‑524749 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |