Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	537,451	T→C	7.0%	noncoding (47/66 nt)	PP_mr07 →	P26 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	537,451	0	T	C	7.0%	208.1 / 4.5	71	noncoding (47/66 nt)	PP_mr07	P26 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (33/33);  new base C (2/3);  total (35/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GCTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAC  >  NC_002947/537307‑537594                                                                                                                                                 |                                                                                                                                                   gcTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCtggtg                                                                                                                                                >  2:174417/1‑149 (MQ=255)  ctcctcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCtggtgg                                                                                                                                               <  4:135326/149‑1 (MQ=255)      tcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTa                                                                                                                                           >  1:220904/1‑149 (MQ=255)        aGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAATCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTAt                                                                                                                                          <  7:42751/148‑1 (MQ=255)               aGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCgg                                                                                                                                  >  2:299153/1‑149 (MQ=255)                gCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGc                                                                                                                                 >  8:14679/1‑149 (MQ=255)                   gAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCAACGGCCtt                                                                                                                              >  7:182915/1‑149 (MQ=255)                         gTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑g                                                                                                                       >  3:291607/1‑149 (MQ=255)                           ttCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                         >  6:301841/1‑146 (MQ=255)                           ttCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                         <  5:301841/146‑1 (MQ=255)                              taaaGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAATAAGCAGGCGATAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAcca                                                                                                                                   <  4:189734/132‑2 (MQ=255)                              gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                 <  6:116126/149‑1 (MQ=255)                              gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                 <  7:115775/149‑1 (MQ=255)                                 agaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggt                                                                                                              >  8:119935/1‑149 (MQ=255)                                 agaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggt                                                                                                              <  7:119935/149‑1 (MQ=255)                                  gaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggtg                                                                                                             >  7:173106/1‑149 (MQ=255)                                    agCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATCCCACCGGCCTTTTTCC‑GT‑TATTGGTGGc                                                                                                           <  1:174417/149‑1 (MQ=255)                                        gCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGt                                                                                                       >  7:234809/1‑149 (MQ=255)                                         cTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGGTATATTTGTGGCCgg                                                                                                        >  1:259682/1‑149 (MQ=255)                                            aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAg‑g                                                                                                  >  8:60745/1‑149 (MQ=255)                                            aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAg                                                                                                    <  4:291607/148‑1 (MQ=255)                                              gACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑Gcc                                                                                                >  8:179899/1‑149 (MQ=255)                                                     agaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTccc                                                                                          >  2:63422/1‑148 (MQ=255)                                                       aagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGa                                                                                        >  2:194188/1‑148 (MQ=255)                                                         gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATaa                                                                                     >  8:113510/1‑149 (MQ=255)                                                         gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAccag                                                                                                                                  >  6:149328/1‑105 (MQ=255)                                                         gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAccag                                                                                                                                  <  5:149328/107‑3 (MQ=255)                                                             cTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGTTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAg                                                                                 >  3:176241/1‑149 (MQ=255)                                                             cTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAg                                                                                 <  1:132916/149‑1 (MQ=255)                                                                gaagaaGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGc                                                                                <  5:168451/147‑1 (MQ=255)                                                                  agaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCa                                                                            <  7:179899/149‑1 (MQ=255)                                                                   gaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCaa                                                                           <  5:203096/149‑1 (MQ=255)                                                                     agCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGa                                                                         <  1:194188/149‑1 (MQ=255)                                                                     agCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGGTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGa                                                                         <  3:216648/149‑1 (MQ=255)                                                                       cAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                         <  7:238145/102‑1 (MQ=255)                                                                       cAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                         >  8:238145/1‑102 (MQ=255)                                                                           cgcTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCa                                                                   >  5:100025/1‑149 (MQ=255)                                                                              tAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                >  6:6485/1‑149 (MQ=255)                                                                                aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                <  1:299153/147‑1 (MQ=255)                                                                                aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATgtgg                                                              <  3:132934/149‑1 (MQ=255)                                                                                     gAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgc                                                         <  8:106196/149‑1 (MQ=255)                                                                                      aGCTGAAGTAATCTCGACCTTGCGATGCCAGCCCGACCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCACatgggtgtct                                                        <  5:45879/149‑4 (MQ=255)                                                                                           aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                 >  5:135668/1‑88 (MQ=255)                                                                                           aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                 <  6:135668/88‑1 (MQ=255)                                                                                           aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAGTGCCGGTCCCGATAACGAGCTGCAAGACACCCACatgggtgtcttg                                                      <  1:63422/147‑6 (MQ=255)                                                                                                aTCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAgg                                              >  5:195860/1‑149 (MQ=255)                                                                                                aTCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAg                                               <  8:173106/148‑1 (MQ=255)                                                                                                    cGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaa                                                     <  1:76605/138‑1 (MQ=255)                                                                                                    cGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaa                                                     >  2:76605/1‑138 (MQ=255)                                                                                                     gACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGttt                                         >  1:199782/1‑149 (MQ=255)                                                                                                        cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTTATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCGGATAACGAGCTGCAAGACACGCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                      <  7:60745/149‑1 (MQ=255)                                                                                                        cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAACCCAAGGGGTTTGCa                                      >  6:58364/1‑149 (MQ=255)                                                                                                        cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                      <  2:199782/149‑1 (MQ=255)                                                                                                        cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                      >  4:288711/1‑149 (MQ=255)                                                                                                           gCGATGCCAGCCTGAGCGTTGAGCGAAAGGGTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                     <  4:176241/147‑1 (MQ=255)                                                                                                            cGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGTCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGCCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGa                                   <  5:81803/148‑1 (MQ=255)                                                                                                                gCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTc                              >  5:35827/1‑149 (MQ=255)                                                                                                                   aGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCTCCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                           >  3:275350/1‑149 (MQ=255)                                                                                                                   aGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                           <  7:119319/149‑1 (MQ=255)                                                                                                                    gCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgc                          <  7:14679/149‑1 (MQ=255)                                                                                                                            gTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccg                  >  1:166281/1‑149 (MQ=255)                                                                                                                                aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCcggcgg              >  6:108502/1‑149 (MQ=255)                                                                                                                                aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg              <  5:6485/149‑1 (MQ=255)                                                                                                                                 gCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg              >  8:48620/1‑148 (MQ=255)                                                                                                                                  cGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg              >  6:107056/1‑147 (MQ=255)                                                                                                                                   gAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTAC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg              >  4:285499/1‑146 (MQ=255)                                                                                                                                     aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa         <  2:249731/149‑1 (MQ=255)                                                                                                                                     aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa         <  6:195860/149‑1 (MQ=255)                                                                                                                                     aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa         <  8:234809/149‑1 (MQ=255)                                                                                                                                            aTGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGCCag    <  5:108502/147‑2 (MQ=255)                                                                                                                                              ggCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAc  >  4:254877/1‑147 (MQ=255)                                                                                                                                              ggCCGGTGGCATAAGCCACCAGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa   >  2:271579/1‑146 (MQ=255)                                                                                                                                              ggCCGGTGGCATAAGCCACCAGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa   <  1:271579/146‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GCTCCTCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAC  >  NC_002947/537307‑537594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑