Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	537,458	T→A	7.6%	noncoding (54/66 nt)	PP_mr07 →	P26 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	537,458	0	T	A	7.6%	216.8 / 4.1	74	noncoding (54/66 nt)	PP_mr07	P26 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (33/35);  new base A (3/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACC  >  NC_002947/537312‑537606                                                                                                                                                   |                                                                                                                                                        tcAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTa                                                                                                                                                       >  1:220904/1‑149 (MQ=255)   aGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAATCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTAt                                                                                                                                                      <  7:42751/148‑1 (MQ=255)          aGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCgg                                                                                                                                              >  2:299153/1‑149 (MQ=255)           gCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGc                                                                                                                                             >  8:14679/1‑149 (MQ=255)              gAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCAACGGCCtt                                                                                                                                          >  7:182915/1‑149 (MQ=255)                    gTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑g                                                                                                                                   >  3:291607/1‑149 (MQ=255)                      ttCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                                     <  5:301841/146‑1 (MQ=255)                      ttCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                                     >  6:301841/1‑146 (MQ=255)                         taaaGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAATAAGCAGGCGATAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAcca                                                                                                                                               <  4:189734/132‑2 (MQ=255)                         gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                             <  6:116126/149‑1 (MQ=255)                         gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                             <  7:115775/149‑1 (MQ=255)                            agaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggt                                                                                                                          >  8:119935/1‑149 (MQ=255)                            agaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggt                                                                                                                          <  7:119935/149‑1 (MQ=255)                             gaagCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATtggtg                                                                                                                         >  7:173106/1‑149 (MQ=255)                               agCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATCCCACCGGCCTTTTTCC‑GT‑TATTGGTGGc                                                                                                                       <  1:174417/149‑1 (MQ=255)                                   gCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGt                                                                                                                   >  7:234809/1‑149 (MQ=255)                                    cTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGGTATATTTGTGGCCgg                                                                                                                    >  1:259682/1‑149 (MQ=255)                                       aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAg‑g                                                                                                              >  8:60745/1‑149 (MQ=255)                                       aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAg                                                                                                                <  4:291607/148‑1 (MQ=255)                                         gACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑Gcc                                                                                                            >  8:179899/1‑149 (MQ=255)                                                agaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTccc                                                                                                      >  2:63422/1‑148 (MQ=255)                                                  aagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGa                                                                                                    >  2:194188/1‑148 (MQ=255)                                                    gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATaa                                                                                                 >  8:113510/1‑149 (MQ=255)                                                    gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAccag                                                                                                                                              >  6:149328/1‑105 (MQ=255)                                                    gaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCAccag                                                                                                                                              <  5:149328/107‑3 (MQ=255)                                                        cTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGTTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAg                                                                                             >  3:176241/1‑149 (MQ=255)                                                        cTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAg                                                                                             <  1:132916/149‑1 (MQ=255)                                                           gaagaaGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGc                                                                                            <  5:168451/147‑1 (MQ=255)                                                             agaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCa                                                                                        <  7:179899/149‑1 (MQ=255)                                                              gaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCaa                                                                                       <  5:203096/149‑1 (MQ=255)                                                                agCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGa                                                                                     <  1:194188/149‑1 (MQ=255)                                                                agCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGGTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGa                                                                                     <  3:216648/149‑1 (MQ=255)                                                                  cAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                                     >  8:238145/1‑102 (MQ=255)                                                                  cAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTcc                                                                                                                                     <  7:238145/102‑1 (MQ=255)                                                                      cgcTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCa                                                                               >  5:100025/1‑149 (MQ=255)                                                                         tAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                            >  6:6485/1‑149 (MQ=255)                                                                           aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                            <  1:299153/147‑1 (MQ=255)                                                                           aaGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATgtgg                                                                          <  3:132934/149‑1 (MQ=255)                                                                                gAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgc                                                                     <  8:106196/149‑1 (MQ=255)                                                                                 aGCTGAAGTAATCTCGACCTTGCGATGCCAGCCCGACCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCACatgggtgtct                                                                    <  5:45879/149‑4 (MQ=255)                                                                                      aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                             >  5:135668/1‑88 (MQ=255)                                                                                      aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TAtt                                                                                                                             <  6:135668/88‑1 (MQ=255)                                                                                      aaGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAGTGCCGGTCCCGATAACGAGCTGCAAGACACCCACatgggtgtcttg                                                                  <  1:63422/147‑6 (MQ=255)                                                                                           aTCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAgg                                                          >  5:195860/1‑149 (MQ=255)                                                                                           aTCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAg                                                           <  8:173106/148‑1 (MQ=255)                                                                                               cGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaa                                                                 >  2:76605/1‑138 (MQ=255)                                                                                               cGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCaa                                                                 <  1:76605/138‑1 (MQ=255)                                                                                                gACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGttt                                                     >  1:199782/1‑149 (MQ=255)                                                                                                   cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTTATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCGGATAACGAGCTGCAAGACACGCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                                  <  7:60745/149‑1 (MQ=255)                                                                                                   cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAACCCAAGGGGTTTGCa                                                  >  6:58364/1‑149 (MQ=255)                                                                                                   cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                                  >  4:288711/1‑149 (MQ=255)                                                                                                   cTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCa                                                  <  2:199782/149‑1 (MQ=255)                                                                                                      gCGATGCCAGCCTGAGCGTTGAGCGAAAGGGTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCAc                                                 <  4:176241/147‑1 (MQ=255)                                                                                                       cGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGTCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGCCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGa                                               <  5:81803/148‑1 (MQ=255)                                                                                                           gCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTc                                          >  5:35827/1‑149 (MQ=255)                                                                                                              aGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCTCCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                                       >  3:275350/1‑149 (MQ=255)                                                                                                              aGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                                       <  7:119319/149‑1 (MQ=255)                                                                                                               gCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgc                                      <  7:14679/149‑1 (MQ=255)                                                                                                                       gTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccg                              >  1:166281/1‑149 (MQ=255)                                                                                                                           aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCcggcgg                          >  6:108502/1‑149 (MQ=255)                                                                                                                           aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          <  5:6485/149‑1 (MQ=255)                                                                                                                            gCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          >  8:48620/1‑148 (MQ=255)                                                                                                                             cGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          >  6:107056/1‑147 (MQ=255)                                                                                                                              gAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTAC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          >  4:285499/1‑146 (MQ=255)                                                                                                                                aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     <  2:249731/149‑1 (MQ=255)                                                                                                                                aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     <  6:195860/149‑1 (MQ=255)                                                                                                                                aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     <  8:234809/149‑1 (MQ=255)                                                                                                                                       aTGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTTCTCCCGGCGGGAGCAGCCag                <  5:108502/147‑2 (MQ=255)                                                                                                                                         ggCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAAc              >  4:254877/1‑147 (MQ=255)                                                                                                                                         ggCCGGTGGCATAAGCCACCAGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa               >  2:271579/1‑146 (MQ=255)                                                                                                                                         ggCCGGTGGCATAAGCCACCAGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCaaa               <  1:271579/146‑1 (MQ=255)                                                                                                                                          gccggtggCATAAGCCACCAGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATCTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGCAGAAGCCAAACaac           >  6:101969/4‑148 (MQ=255)                                                                                                                                              gtggCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg        <  5:107056/148‑1 (MQ=255)                                                                                                                                                  cTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGAc   <  6:35827/149‑1 (MQ=255)                                                                                                                                                   ttATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCC‑GGAGAAGCCAAACAAGCAGGTGAcc  >  5:112169/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                        TCAGGTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCC‑GT‑TATTGGTGGCCGGTCAG‑GCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACC  >  NC_002947/537312‑537606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑